Structure and Antioxidant Activity of Polysaccharides Extracted from <i>Sargassum fusiforme</i>

WU Juan; OU Zhi-rong; LI Zhao-rong; ZHAO Mou-ming

doi:10.19303/j.issn.1008-0384.2019.07.014

Volume 34 Issue 7

Jul. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(7): 842-851

WU Juan, OU Zhi-rong, LI Zhao-rong, ZHAO Mou-ming. Structure and Antioxidant Activity of Polysaccharides Extracted from Sargassum fusiforme[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 842-851. doi: 10.19303/j.issn.1008-0384.2019.07.014

Citation:

WU Juan, OU Zhi-rong, LI Zhao-rong, ZHAO Mou-ming. Structure and Antioxidant Activity of Polysaccharides Extracted from Sargassum fusiforme[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 842-851. doi: 10.19303/j.issn.1008-0384.2019.07.014

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Structure and Antioxidant Activity of Polysaccharides Extracted from Sargassum fusiforme

doi: 10.19303/j.issn.1008-0384.2019.07.014

School of Food Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510640, China

Received Date: 2019-02-12
Rev Recd Date: 2019-05-14
Publish Date: 2019-07-20

Abstract

Abstract

Objective The polysaccharides extracted from Sargassum fusiforme with different ethanol volume were studied. Method Polysaccharides were extracted from S.fusiforme using an acid solution, then precipitated with different volume fractions of ethanol. The resulting extract was analyzed for its physicochemical properties, monosaccharide composition, molecular weight, structural characteristics, functional group identification, spatial conformation, apparent morphology, and rheological properties using ultraviolet spectrophotometer, precolumn derivatization-reverse-phase high performance liquid chromatography, GPC-MALLs, FT-IR, SEM, and Huck MARS Ⅲ rheometer. The antioxidant activity of the extracts, including the oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability, were determined. Result Extracts, SFP-50 and SFP-70, were found to contain higher contents of sugar, sulfate and uronic acid than the other samples. All 5 extracts contained mannose, glucose, glucuronic acid, galactose, arabinose, and fucose but differed in ratio, with fucose being the highest at about 50% of them combined. The molecular weights of SFP-40, SFP-50, SFP-60, SFP-70, and SFP-80 were estimated to be 97.63, 90.51, 97.19, 81.52, and 90.93 kDa, respectively. The Congo red assay showed that the polysaccharides had a triple helical structure. The solution of the extracts was non-Newtonian fluid with elasticity. The scanning electron microscopy images showed an uneven, loose and porous surface. All samples had varying degrees of oxygen radical absorption capacity and DPPH· and ABTS· scavenging ability. Conclusion The volume fraction employed for the ethanol precipitation affected the physicochemical properties, monosaccharide composition, and molecular weight as well as the antioxidant activity, but not the conformation, microstructure or rheological properties, of the polysaccharides obtained.
- Sargassum fusiforme,
- polysaccharides,
- structural characterization,
- antioxidant activity

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References(31)

References

[1]	刘洪超, 应苗苗, 周雨暪, 等.羊栖菜多糖提取条件优化及其抗氧化活性的研究[J].食品工业科技, 2017, 38(6):245-249. http://d.old.wanfangdata.com.cn/Periodical/spgykj201706054 LIU H C, YING M M, ZHOU Y M, et al. Optimization of Extraction Conditions and Antioxidant Activity of Polysaccharide from Sargassum fusiformis[J]. Food Science and Technology, 2017, 38(6):245-249.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spgykj201706054
[2]	SUN Y, CHEN X, ZHANG L, et al. The antiviral property of Sargassum fusiforme polysaccharide for avian leukosis virus subgroup J in vitro and in vivo[J]. International Journal of Biological Macromolecules, 2019, 138:70-78.
[3]	LI Y, CHEN B, WU W, et al. Antioxidant and antimicrobial evaluation of carboxymethylated and hydroxamated degraded polysaccharides from Sargassum fusiforme[J]. International Journal of Biological Macromolecules, 2018, 118:1550-1557. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=f4e2c4cfc4c6773aac0d5f6119524fa2
[4]	FAN S, YU G, NIE W, et al. Antitumor activity and underlying mechanism of Sargassum fusiforme polysaccharides in CNE-bearing mice[J]. International Journal of Biological Macromolecules, 2018, 112:516-522. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=56cf24327d0cd813e612c4639ae58b94
[5]	CHEN L, CHEN P, LIU J, et al. Sargassum Fusiforme Polysaccharide SFP-F2 Activates the NF-κB Signaling Pathway via CD14/IKK and P38 Axes in RAW264.7 Cells[J]. Marine Drugs, 2018, 16(8):264.
[6]	张胜帮, 麻卫锋, 于萍.羊栖菜多糖提取分离及其清除自由基的活性研究[J].食品科学, 2009, 30(18):192-195. http://d.old.wanfangdata.com.cn/Periodical/spkx200918040 ZHANG S B, MA W F, YU P. Extraction and Separation of Polysaccharide from Sargassum fusiformis and Its Activity of Free Radical Scavenging[J]. Food Science, 2009, 30(18):192-195.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spkx200918040
[7]	季宇彬, 杨书良, 谷春山.羊栖菜多糖对L(615)小鼠LPO含量及GR、GSH-PX、CAT和SOD活性的影响[J].中国海洋药物. 1994(2):20-23. http://www.cnki.com.cn/Article/CJFD1994-HYYW199402003.htm JI Y B, YANG S L, GU C S. Effect of sargassum polysaccharide on LPO Content and Activity of GR, GSH-PX, CAT and SOD in L(615) Mice[J]. Chinese marine medicine, 1994(2):20-23.(in Chinese) http://www.cnki.com.cn/Article/CJFD1994-HYYW199402003.htm
[8]	陈金星, 胡昔城, 杨维, 等.羊栖菜多糖体外诱导人大肠癌细胞凋亡[J].基础医学与临床, 2008(2):153-159. http://d.old.wanfangdata.com.cn/Periodical/jcyxylc200802009 CHEN J X, HU X C, YANG W, et al. Sargassum polysaccharide induces apoptosis of human colorectal cancer cells in vitro[J]. Basic medicine and clinical, 2008(2):153-159.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jcyxylc200802009
[9]	王扬, 何良强, 王海洪, 等.羊栖菜多糖对小鼠免疫功能的影响[J].宁波大学学报(理工版). 2003(3):245-247. http://d.old.wanfangdata.com.cn/Periodical/nbdxxb-lg200303008 WANG Y, HE L Q, WANG H H, et al. Effect of sargassum polysaccharide on immune function in mice[J]. Journal of Ningbo University (Technology Edition), 2003(3):245-247.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/nbdxxb-lg200303008
[10]	于竹芹, 于雪莲, 李生尧, 等.羊栖菜对四氧嘧啶糖尿病模型小鼠血糖水平影响[J].青岛大学医学院学报, 2011(1):28-30. http://d.old.wanfangdata.com.cn/Periodical/qdyxyxb201101010 YU Z Q, YU X L, LI S Y, et al. Effect of sargassum on blood glucose level in alloxan diabetic model mice[J]. Journal of Qingdao University Medical College, 2011(1):28-30. http://d.old.wanfangdata.com.cn/Periodical/qdyxyxb201101010
[11]	GAO J, LIN L L, ZHAO M M, et al. A comparison study on polysaccharides extracted from Laminaria japonica using different methods structural characterization and bile acid-binding capacity[J]. Food & Function, 2017(8):3043-3052. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=c62649e9feea35e062a4c68d7be214df
[12]	DUBOIS M, GILLES K A, HAMILTON J K, et al. Colorimetric Method for Determination of Sugars and Related Substances[J]. Analytical Chemistry, 1956, 28(3):350-356. doi: 10.1021-ac60111a017/
[13]	王新宇, 刘振华, 李影丹, 等.猴头菌浸膏多糖及蛋白质含量测定方法的比较[J].黑龙江畜牧兽医, 2018(21):219-223. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hljxmsy201811061 WANG X Y, LIU Z H, LI Y D, et al. Comparison of methods for determination of polysaccharides and protein content in Hericium erinaceus extracts[J]. Heilongjiang Animal Husbandry and Veterinary Medicine, 2018(21):219-223.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hljxmsy201811061
[14]	KPODO F M, AGBENORHEVI J K, ALBA K, et al. Structure and physicochemical properties of Ghanaian grewia gum[J]. International Journal of Biological Macromolecules, 2019, 122:866-872. http://cn.bing.com/academic/profile?id=4afc2c3220aa1d14f9249f4411744328&encoded=0&v=paper_preview&mkt=zh-cn
[15]	丛建波, 王长振, 李妍, 等.褐藻硫酸多糖硫酸基含量测定——硫酸钡比浊法研究[J].解放军药学学报, 2003(3):181-183. http://d.old.wanfangdata.com.cn/Periodical/jfjyxxb200303008 CONG J B, WANG C Z, LI Y, et al. Determination of Sulfate Content in Brown Algae Sulfated Polysaccharide -Study on Turbidimetry of Barium Sulfate[J]. Chinese Journal of Pharmaceutical Sciences, 2003(3):181-183.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jfjyxxb200303008
[16]	LIU Z, PI F, GUO X, et al. Characterization of the structural and emulsifying properties of sugar beet pectins obtained by sequential extraction[J]. Food Hydrocolloids, 2019, 88:31-42. http://cn.bing.com/academic/profile?id=4df1ec2f8ae222aef43e9ebe2a2c2618&encoded=0&v=paper_preview&mkt=zh-cn
[17]	戴军, 朱松, 汤坚, 等. PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖的单糖组成[J].分析测试学报, 2007(2):206-210. http://d.old.wanfangdata.com.cn/Periodical/fxcsxb200702015 DAI J, ZHU S, TANG J, et al. Analysis of Monosaccharide Composition of Dunaliella Salina Polysaccharide by PMP Pre-column Derivatization High Performance Liquid Chromatography[J]. Journal of Instrumental Analysis, 2007(2):206-210.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/fxcsxb200702015
[18]	CHEN C, ZHANG B, HUANG Q, et al. Microwave-assisted extraction of polysaccharides from Moringa oleifera Lam. leaves:Characterization and hypoglycemic activity[J]. Industrial Crops and Products, 2017, 100:1-11. http://cn.bing.com/academic/profile?id=7f2b88e6f093ee49171b45575ebaa40a&encoded=0&v=paper_preview&mkt=zh-cn
[19]	林恋竹, 朱启源, 赵谋明.辣木籽抗氧化肽的制备及其对氧化损伤红细胞的保护作用[J].食品科学, 2019, 40(7):40-46. http://d.old.wanfangdata.com.cn/Periodical/spkx201907007 LIN L Z, ZHU Q Y, ZHAO M M. Preparation of Moringa Seed Antioxidant Peptide and Its Protective Effect on Oxidative Damaged Red Blood Cells[J]. Food Science, 2019, 40(7):40-46.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spkx201907007
[20]	LI C, HUANG Q, FU X, et al. Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn[J]. Int J Biol Macromol, 2015, 75:298-305. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=61892885076d7e36fe5f2bd1212b898d
[21]	赵谋明, 董红竹, 林恋竹.八种水果多酚的定量分析与抗氧化活性研究[J].现代食品科技, 2017, 33(10):225-236. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201710032 ZHAO M M, DONG H Z, LIN L Z. Quantitative Analysis and Antioxidant Activity of Eight Kinds of Fruit Polyphenols[J]. Journal of Modern Food Science, 2017, 33(10):225-236.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201710032
[22]	田鑫, 李秀霞, 吴科阳, 等.海藻多糖提取纯化及生物活性的研究进展[J].食品与发酵科技, 2015, 51(6):81-85. http://d.old.wanfangdata.com.cn/Periodical/scspyfx201506018 TIAN X, LI X X, WU K Y, et al. Research progress in extraction, purification and biological activity of seaweed polysaccharides[J]. Food Science ang Technology, 2015, 51(6):81-85.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/scspyfx201506018
[23]	卫阳飞, 宋海, 宗盈晓, 等.分级醇沉柳茶多糖的含量与单糖组成及抗氧化性研究[J].华西药学杂志, 2018, 33(6):489-493. http://d.old.wanfangdata.com.cn/Periodical/hxyxzz201806008 WEI Y F, SONG H, ZONG Y X, et al. Study on the content of polysaccharides, monosaccharide composition and antioxidant activity of fractionated alcoholic willow tea[J]. West China Journal of Pharmaceutical Sciences, 2018, 33(6):489-493.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hxyxzz201806008
[24]	YE H, WANG K, ZHOU C, et al. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum[J]. Food Chem, 2008, 111(2):428-432. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=2792d77c7798c22fbf6c5acd56dd5a6a
[25]	戴伟, 刘新义, 胡雄彬, 等.香菇多糖的分子量和结构与生物活性之间的关系[J].中南药学, 2012, 10(6):453-456. http://d.old.wanfangdata.com.cn/Periodical/znyx201206017 DAI W, LIU X Y, HU X B, et al. Relationship between molecular weight and structure and bioactivity of Lentinus edodes polysaccharides[J]. Zhongnan Pharmaceutical, 2012, 10(6):453-456.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/znyx201206017
[26]	田瑞, 程超, 冯佳, 等.三百棒多糖的流变学特性及抗氧化活性研究[J].中国现代应用药学, 2018, 35(11):1656-1659. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgxdyyyx201811014 TIAN R, CHENG C, FENG J, et al. Study on Rheological Properties and Antioxidant Activity of Polysaccharides from Three Bars[J]. Contemporary Chinese application, 2018, 35(11):1656-1659.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgxdyyyx201811014
[27]	扶雄, 李湘师, 游丽君, 等.不同提取方法对海蒿子多糖性质的影响研究[J].食品工业科技, 2015, 36(9):101-106. http://d.old.wanfangdata.com.cn/Periodical/spgykj201509023 FU X, LI X S, YOU L J, et al. Effects of different extraction methods on the polysaccharide properties of Artemisia scoparia[J]. Food Science and Technology, 2015, 36(9):101-106.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spgykj201509023
[28]	吴慧, 卢虹玉, 李梅婷, 等.亨氏马尾藻醇提取物及其不同溶剂萃取物的抗氧化活性研究[J].食品工业科技, 2016, 37(10):171-174. http://d.old.wanfangdata.com.cn/Periodical/spgykj201610038 WU H, LU H Y, LI M T, et al. Antioxidant Activity of Extracts from Hem's Algae and Its Different Solvent Extracts[J]. Food Science and Technology, 2016, 37(10):171-174.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spgykj201610038
[29]	李杰女, 汲晨锋, 季宇彬.羊栖菜多糖药用活性的研究进展[J].亚太传统医药, 2009, 5(7):148-150. http://d.old.wanfangdata.com.cn/Periodical/ytctyy200907082 LI J N, QI C F, JI Y B. Research progress on the medicinal activity of polysaccharides from Sargassum fusiformis[J]. Asia-Pacific Traditional Medicine, 2009, 5(7):148-150.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ytctyy200907082
[30]	WU S, ZHANG X, LIU J, et al. Physicochemical characterization of Sargassum fusiforme fucoidan fractions and their antagonistic effect against P-selectin-mediated cell adhesion[J]. International Journal of Biological Macromolecules, 2019, 133:656-662. http://cn.bing.com/academic/profile?id=12ba3a9b4467435003ecf4507a0401db&encoded=0&v=paper_preview&mkt=zh-cn
[31]	SUN Y, CHEN X, LIU S, et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. Journal of Oceanology and Limnology, 2018, 36(3):882-891. http://d.old.wanfangdata.com.cn/Periodical/zghyhzxb201803026