A Study on Ligninolytic Enzyme-producing Fungi

YUAN Hai-hua; ZHANG Bao; ZHANG Ying; AO Xin-yu

doi:10.19303/j.issn.1008-0384.2019.07.012

Volume 34 Issue 7

Jul. 2019

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YUAN Hai-hua, ZHANG Bao, ZHANG Ying, AO Xin-yu. A Study on Ligninolytic Enzyme-producing Fungi[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 829-836. doi: 10.19303/j.issn.1008-0384.2019.07.012

Citation:

YUAN Hai-hua, ZHANG Bao, ZHANG Ying, AO Xin-yu. A Study on Ligninolytic Enzyme-producing Fungi[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 829-836. doi: 10.19303/j.issn.1008-0384.2019.07.012

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A Study on Ligninolytic Enzyme-producing Fungi

doi: 10.19303/j.issn.1008-0384.2019.07.012

1.
College of Life Science, Southwest Forestry University, Kunming, Yunnan 650224, China
2.
College of Biodiversity Conservation and Utilization, Southwest Forestry University, Kunming, Yunnan 650224, China

Received Date: 2019-03-15
Rev Recd Date: 2019-06-22
Publish Date: 2019-07-20

Abstract

Abstract

Objective To search for wood-rotting fungi that are highly effective in producing ligninolytic enzymes(LMLs), such asLac, MnP, and LiP. Methods Nine strains of wood-rotting fungi with known ability to produce LMLs were screened and their biological characteristics studied. Results The strains with high capacity of generating LMLs and their yields on each of the LMLs were found to be:LS136 (yielding Lac at 0.18 U·L^-1, LiP at 109.68 U·L^-1, and MnP at 140.38 U·L^-1), LJ485 (yielding Lac at 136.99 U·L^-1, LiP at 9.53 U·L^-1, and MnP at 72.05 U·L^-1), and LJ496 (yielding Lac at 163.39 U·L^-1, LiP at 9.50 U·L^-1, and MnP at 74.36 U·L^-1). The optimum growth temperature for the 3 fungal strains was approximately 25℃, and pH ranged 4 to 8. The strains were all highly tolerant to Cr³⁺ or Pb²⁺ at concentrations of 0-8 mmoL·L^-1 and Cu²⁺ at 0-4 mmoL·L^-1. At the concentrations between 1-4 mmoL·L^-1, Cr³⁺ promoted the growth of LJ485 and LJ496. Conclusion The identified LS136 was thought to be a Stereum sp., LJ485 identified as Trametes versicolor, and LJ496 considered an Antrodiella sp.. They all demonstrated varying capacities of producing LMLs with a tolerance to heavy metals. The information would be useful for further investigation on the utilization of these lignin degrading fungi.
- wood-rotting fungus,
- ligninolytic enzymes,
- biological characteristics

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References(18)

References

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