Response Surface Optimization of Flavonoid Extraction and <i>in Vitro</i> Antioxidant Activity of Extract from <i>Dendrobium Chrysotoxum</i> Lindl. Flowers

WANG Yan-bing; HUANG Jia-wei; LI Guo-ming; LIU Xiao-qiong; HU Yong-liang; WANG Xiao-yuan; BAI Yan-bing

doi:10.19303/j.issn.1008-0384.2019.06.015

Volume 34 Issue 6

Sep. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(6): 730-738

WANG Yan-bing, HUANG Jia-wei, LI Guo-ming, LIU Xiao-qiong, HU Yong-liang, WANG Xiao-yuan, BAI Yan-bing. Response Surface Optimization of Flavonoid Extraction and in Vitro Antioxidant Activity of Extract from Dendrobium Chrysotoxum Lindl. Flowers[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 730-738. doi: 10.19303/j.issn.1008-0384.2019.06.015

Citation:

WANG Yan-bing, HUANG Jia-wei, LI Guo-ming, LIU Xiao-qiong, HU Yong-liang, WANG Xiao-yuan, BAI Yan-bing. Response Surface Optimization of Flavonoid Extraction and in Vitro Antioxidant Activity of Extract from Dendrobium Chrysotoxum Lindl. Flowers[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 730-738. doi: 10.19303/j.issn.1008-0384.2019.06.015

Citation:

PDF( 3807 KB)

Response Surface Optimization of Flavonoid Extraction and in Vitro Antioxidant Activity of Extract from Dendrobium Chrysotoxum Lindl. Flowers

doi: 10.19303/j.issn.1008-0384.2019.06.015

Dehong Tropical Agriculture Institute of Yunnan, Ruili, Yunnan 678600, China

Received Date: 2019-02-12
Rev Recd Date: 2019-05-14
Publish Date: 2019-06-28

Abstract

Abstract

Objective Extraction process of flavonoids from the flowers of Dendrobium chrysotoxum Lindl. was optimized, and the antioxidative activity of the extract determined in vitro. Method Flowers of D. chrysotoxum was subjected to an ethanol extraction for flavonoids. The resulting yield was weighed against the processing conditions based on the single-factor tests and Box-Benhnken center composite experiment with four factors including ethanol volume fraction, solvent-to-substrate ratio, temperature, and time of ultrasonic treatment. The antioxidant capacity was estimated by the ability of the extract to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl free radicals. Result The optimized processing conditions were determined to include ethanol volume fraction at 83%, solvent-to-substrate ratio at 31 mL·g^-1, temperature at 59℃ and ultrasound application for 44m to obtain up to 9.71 mg·g^-1 of total flavonoids in the extract. The yield exceeded the theoretically expected 9.60 mg·g^-1 with a relative deviation of 1.15%. The in vitro tests showed an IC₅₀ for DPPH to be 11.30 μg·mL^-1, a scavenging ability 0.51-time of L-ascorbic acid or 1.42-time of BHT, while that for hydroxyl radicals, 137.26 μg·mL^-1, a scavenging ability 0.70-time of L-ascorbic acid or 1.95-time of BHT. Conclusion The optimized flavonoid extraction paved the way for scale-up utilization of D. chrysotoxum flowers.
- Dendrobium chrysotoxum Lindl. flowers,
- total flavonoids,
- process optimization,
- response surface analysis,
- antioxidant

FullText(HTML)

References(29)

References

[1]	肖春宏, 黄飞燕, 杨波.鼓槌石斛研究进展[J].山西农业科学, 2014, 42(6):647-649. doi: 10.3969/j.issn.1002-2481.2014.06.29 XIAO C H, HUANG F Y, YANG B. Study progress in Dendronbium chrysotoxum Lindl[J]. Journal of Shanxi Agricultural Sciences, 2014, 42(6):647-649.(in Chinese) doi: 10.3969/j.issn.1002-2481.2014.06.29
[2]	龚庆芳, 黄宁珍, 何金祥, 等.不同光照强度对鼓槌石斛生长特性和化学成分的影响[J].北方园艺, 2015(23):155-159. http://d.old.wanfangdata.com.cn/Periodical/bfyany201523044 GONG Q F, HUANG N Z, HE J X, et al. Effect of different light intensity on the growth characteristics and chemical constituents of Dendrobium chrysotoxum Lindl[J]. Northern Horticulture, 2015(23):155-159.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201523044
[3]	唐德英, 马洁, 张丽霞, 等.鼓槌石斛种质资源调查研究[J].中国中药杂志, 2010, 35(12):1529-1532. http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201012005 TANG D Y, MA J, ZHANG L X, et al. Research and investigation in germplasm resource of Dendrobium chrysotoxum[J]. China Journal of Chinese Materia Medica, 2010, 35(12):1529-1532.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201012005
[4]	赵燕宜, 范志霞, 黄坤, 等.中华人民共和国药典(一部)[S].北京: 中国医药科技出版社, 2015: 282-283. ZHAO Y Y, FAN Z X, HUANG K, et al. Pharmacopoeia of the people's republic of china(part Ⅰ)[S]. Beijing: China Medical Science Press, 2015: 282-283.(in Chinese)
[5]	MA G X, LEBlANC G A. The activity of erianin and chrysotoxine from Dendrobium chrysotoxum to reverse multidrug resistance in B16/h MDR-1 cells[J]. Journal of Chinese Pharmaceutical Sciences, 1998, 7:142-146. http://www.cnki.com.cn/Article/CJFDTotal-XYGZ803.005.htm
[6]	PAN L H, WANG J, YE X Q, et al. Enzyme-assisted extraction of polysaccharides from Dendrobium chrysotoxum and its functional properties and immunomodulatory activity[J]. LWT-Food Science and Technology, 2015, 60(2):1149-1154. doi: 10.1016/j.lwt.2014.10.004
[7]	余增洋, 盛雨辰, 周玲玉, 等.鼓槌石斛对小鼠非增殖性糖尿病视网膜病变的改善作用及其机制研究[J].中草药, 2016, 47(11):1908-1913. doi: 10.7501/j.issn.0253-2670.2016.11.016 YU Z Y, SHENG Y C, ZHOU L Y, et al. Study on amelioration of Dendrobium chrysotoxum on STZ-induced non-proliferative diabetic retinopathy and its engaged mechanism[J]. Chinese Traditional and Herbal Drugs, 2016, 47(11):1908-1913.(in Chinese) doi: 10.7501/j.issn.0253-2670.2016.11.016
[8]	文小玲, 王娅娟, 徐俊驹, 等.云南鼓槌石斛提取物体外自由基清除活性研究[J].昆明医科大学学报, 2014, 35(12):4-6. doi: 10.3969/j.issn.1003-4706.2014.12.002 WEN X L, WANG Y J, XU J J, et al. In vitro radical scavenging activity of extracts from Dendrobium chrysotoxum in Yunnan[J]. Journal of Kunming Medical University, 2014, 35(12):4-6.(in Chinese) doi: 10.3969/j.issn.1003-4706.2014.12.002
[9]	张冬英, 范黎明, 龚舒静, 等.鼓槌石斛花总黄酮及挥发性成分研究[J].食品科技, 2014, 39(10):198-202. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spkj201410042 ZHANG D Y, FAN L M, GONG S J, et al. Total flavonoids and essential oil of Dendrobium chrysotoxum flower[J]. Food Science and Technology, 2014, 39(10):198-202.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=spkj201410042
[10]	黄秀红, 王再花, 李杰, 等.不同花期石斛花主要营养成分分析与品质比较[J].热带作物学报, 2017, 38(1):45-52. doi: 10.3969/j.issn.1000-2561.2017.01.009 HUANG X H, WANG Z H, LI J, et al. Comparative analysis of quality properties and main nutrients in Dendrobium flowers during different flowering phases[J]. Chinese Journal of Tropical Crops, 2017, 38(01):45-52.(in Chinese) doi: 10.3969/j.issn.1000-2561.2017.01.009
[11]	王毅, 孙永玉, 李昆, 等.一种鼓槌石斛花茶的制作方法: CN103689169A[P]. 2014-04-02. WANG Y, SUN Y Y, LI K, et al. Dendrobium chrysotoxum Lindl. Flowers tea production method: CN103689169A[P]. 2014-04-02.(in Chinese)
[12]	LI H, HAO Z, WANG X, et al. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance[J]. Bioresource technology, 2009, 100(2):970-974. doi: 10.1016/j.biortech.2008.07.021
[13]	赵美莲, 于春艳, 史晓晶.水蜡树果实总黄酮提取工艺优化及其抗氧化活性[J].福建农业学报, 2018, 33(2):206-211. doi: 10.19303/j.issn.1008-0384.2018.02.018 ZHAO M L, YU C Y, SHI X J, et al. Optimization and antioxidant activity of flavonoids extracted from fruits of Ligustrum obtusifolium[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2):206-211.(in Chinese) doi: 10.19303/j.issn.1008-0384.2018.02.018
[14]	周锦业, 孙明艳, 邓杰玲, 等.品种、干燥和贮存方式对铁皮石斛花氨基酸含量的影响[J].食品科技, 2019, 44(2):88-93. ZHOU J Y, SUN M Y, DENG J L, et al. Effects of different factors on the amino acid content of Dendrobium officinale flower[J]. Food Science and Technology, 2019, 44(2):88-93.(in Chinese)
[15]	梁凯伦, 方萍, 施秋秋, 等.铁皮石斛花对高糖高脂饮酒致高血压大鼠的降压作用及机制研究[J].中国中药杂志, 2018, 43(1):147-153. http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201801022 LIANG K L, FANG P, SHI Q Q, et al. Antihypertensive effect and mechanism of Dendrobium officinale flos on high-blood pressure rats induced by high glucose and high fat compound alcohol[J]. China Journal of Chinese Materia Medica, 2018, 43(1):147-153.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201801022
[16]	何晓艳, 吴人照, 龙华晴, 等.铁皮石斛花对自发性高血压大鼠的降压作用及机制研究[J].中华中医药杂志, 2017, 32(4):1836-1840. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgyyxb201704123 HE X Y, WU R Z, LONG H Q, et al. Study on antihypertensive effect and mechanism of Dendrobium flowers in spontaneously hypertensive rats[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2017, 32(4):1836-1840.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgyyxb201704123
[17]	张聪, 刘守金, 杨柳, 等. GC-MS法检测云南产细茎石斛花中挥发性成分[J].云南农业大学学报(自然科学), 2017, 32(1):174-178. http://www.cqvip.com/QK/94499X/201701/671406430.html ZHANG C, LIU S J, YANG L, et al. Determination of volatile components from flowers of Dendrobium moniliforme (L.) Sw in Yunnan by GC-MS[J]. Journal of Yunnan Agricultural University(Natural Science), 2017, 32(1):174-178.(in Chinese) http://www.cqvip.com/QK/94499X/201701/671406430.html
[18]	李文静, 李进进, 李桂锋, 等. GC-MS分析4种石斛花挥发性成分[J].中药材, 2015, 38(4):777-780. http://d.old.wanfangdata.com.cn/Periodical/zyc201504032 LI W J, LI J J, LI G F, et al. Determination of volatile components from 4 Dendrobium flowers by GC-MS[J]. Journal of Chinese Medicinal Materials, 2015, 38(4):777-780.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zyc201504032
[19]	唐静月, 颜美秋, 齐芳芳, 等.铁皮石斛花总黄酮提取工艺优化及体外抗氧化活性研究[J].浙江中医药大学学报, 2017, 41(3):235-242. http://d.old.wanfangdata.com.cn/Periodical/zjzyxyxb201703017 TANG J Y, YAN M Y, QI F F, et al. Study on optimum extraction of total flavones in Dendrobium officinale flowers and its antioxidant activity in vitro[J]. Journal of Zhejiang Chinese Medical University, 2017, 41(3):235-242.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjzyxyxb201703017
[20]	缪园欣, 廖明星, 孙爱红, 等.超声-乙醇法提取铁皮石斛花总黄酮及其体外抗氧化性的研究[J].中国酿造, 2019, 38(4):155-159. http://d.old.wanfangdata.com.cn/Periodical/zgnz201904030 MIAO Y X, LIAO M X, SUN A H, et al. Extraction of total flavonoids from Dendrobium officinale flowers by ultrasonic-ethanol synergistic and its antioxidant activity[J]. China Brewing, 2019, 38(4):155-159.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgnz201904030
[21]	LAGUERRE M, HUGOUVIEUX V, CAVUSOGLU N, et al. Probing the micellar solubilisation and inter-micellar exchange of polyphenols using the DPPH free radical[J]. Food chemistry, 2014, 149:114-120. doi: 10.1016/j.foodchem.2013.10.034
[22]	符群, 吴桐, 王梦丽.负压超声法提取刺玫果黄酮及其抗氧化性研究[J].现代食品科技, 2019, 35(1):165-172. http://www.cnki.com.cn/Article/CJFDTotal-GZSP201901024.htm FU Q, WU T, WANG M L. Study on extraction and antioxidant activity of flavonoids from Rosa davurica Pall. by negative pressure ultrasound[J]. Modern Food Science and Technology, 2019, 35(1):165-172.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-GZSP201901024.htm
[23]	张黎明, 李瑞超, 郝利民, 等.响应面优化玛咖叶总黄酮提取工艺及其抗氧化活性研究[J].现代食品科技, 2014, 30(4):233-239. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201404039 ZHANG L M, LI R C, HAO L M, et al. Response surface methodology for optimization of extracting total flavonoids from Maca leaves and antioxidant evaluation[J]. Modern Food Science and Technology, 2014, 30(4):233-239.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201404039
[24]	WANG Y, GAO Y, DING H, et al. Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity[J]. Food chemistry, 2017, 218:152-158. doi: 10.1016/j.foodchem.2016.09.058
[25]	李加兴, 陈选, 邓佳琴, 等.黄秋葵黄酮的提取工艺和体外抗氧化活性研究[J].食品科学, 2014, 35(10):121-125. doi: 10.7506/spkx1002-6630-201410022 LI J X, CHEN X, DENG J Q, et al. Extraction and antioxidant activity in vitro of Okra flavonoids[J]. Food Science, 2014, 35(10):121-125.(in Chinese) doi: 10.7506/spkx1002-6630-201410022
[26]	磨正遵, 商飞飞, 潘中田, 等.响应面法优化超声波辅助提取广西大果山楂叶黄酮工艺[J].南方农业学报, 2018, 49(5):986-992. doi: 10.3969/j.issn.2095-1191.2018.05.23 MO Z Z, SHANG F F, PAN Z T, et al. Optimization of ultrasonic-assisted extraction of flavonoids from Guangxi big fruit hawthorn leaves by response surface methodology[J]. Journal of Southern Agriculture, 2018, 49(5):986-992.(in Chinese) doi: 10.3969/j.issn.2095-1191.2018.05.23
[27]	WANG Y, CHEN P, TANG C, et al. Antinociceptive and anti-inflammatory activities of extract and two isolated flavonoids of Carthamus tinctorius L[J]. Journal of ethnopharmacology, 2014, 151(2):944-950. doi: 10.1016/j.jep.2013.12.003
[28]	LI J, LIU Y, FAN L, et al. Antioxidant activities of polysaccharides from the fruiting bodies of Zizyphus Jujuba cv. Jinsixiaozao[J]. Carbohydrate Polymers, 2011, 84(1):390-394. doi: 10.1016/j.carbpol.2010.11.051
[29]	熊双丽, 卢飞, 史敏娟, 等. DPPH自由基清除活性评价方法在抗氧化剂筛选中的研究进展[J].食品工业科技, 2012, 33(8):380-383. http://d.old.wanfangdata.com.cn/Periodical/spgykj201208123 XIONG S L, LU F, SHI M J, et al. Advanement of evaluation methods about DPPH radical scavenging activity in Screening Antioxidant[J]. Food Science, 2012, 33(8):380-383.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spgykj201208123