Multi-gene Transformation of Indica Rice, Minghui 86

WEI Lin-yan; LIAN Ling; WEI Yi-dong; LUO Xi; HE Wei; XIE Hong-guang; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2019.06.001

Volume 34 Issue 6

Sep. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(6): 621-629

WEI Lin-yan, LIAN Ling, WEI Yi-dong, LUO Xi, HE Wei, XIE Hong-guang, XIE Hua-an, ZHANG Jian-fu. Multi-gene Transformation of Indica Rice, Minghui 86[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 621-629. doi: 10.19303/j.issn.1008-0384.2019.06.001

Citation:

WEI Lin-yan, LIAN Ling, WEI Yi-dong, LUO Xi, HE Wei, XIE Hong-guang, XIE Hua-an, ZHANG Jian-fu. Multi-gene Transformation of Indica Rice, Minghui 86[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 621-629. doi: 10.19303/j.issn.1008-0384.2019.06.001

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Multi-gene Transformation of Indica Rice, Minghui 86

doi: 10.19303/j.issn.1008-0384.2019.06.001

WEI Lin-yan^{1, 2, 3
,},
LIAN Ling^{1, 2, 3},
WEI Yi-dong^{1, 2, 3},
LUO Xi^{1, 2, 3},
HE Wei^{1, 2, 3},
XIE Hong-guang^{1, 2, 3},
XIE Hua-an^{1, 2, 3
,
,},
ZHANG Jian-fu^{1, 2, 3
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rual/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Incubator of National Key Laboratory of Crops Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Science and Technology/Base for South-China, National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian 350003, China
3.
National Engineering Laboratory of Rice of China, Fuzhou, Fujian 350003, China

Received Date: 2019-02-19
Rev Recd Date: 2019-05-05
Publish Date: 2019-06-28

Abstract

Abstract

Objective Conditions for simultaneous transformation of multiple genes in Indica rice, Minghui 86, were studied to facilitate the development of high yield and resistant breeds. Method Two multi-gene vectors designated as P5 and P8 that harbored the high-yield RRM2, drought-tolerant HS1, herbicide-resistant EPSPS, and insect-resistant Bt as well as the apoptosis-inhibiting iap and gene that promotes cell regeneration p35 were transformed to the receptor Minghui 86 using agrobacterium-based transformation methodology. The receptor, callus culture, agrobacterium concentration, infection time, co-culture, and screening concentrations of G418 and glyphosate were evaluated for the selection. Result Under same culture conditions, the recovery rate of Minghui 86 immature embryos was significantly higher with a better callus quality than that of the mature embryos. The optimal transformation conditions were determinated to be a bacterial concentration of OD₆₀₀=0.4-0.6, an infection time of 15-20 m, co-culture for 2-3 d with the addition of sterile filter paper in medium, and the screening concentration of G418 at 150 mg·L^-1 and of glyphosate at 800 mg·L^-1. The PCR detection confirmed that GUS in the polygenic vector P5 was successfully transferred into Indica cv. Minghui 86. Conclusion The optimized culture conditions afforded significantly improved callus and resistance induction rates in Minghui 86.
- gene,
- genetic transformation,
- indica rice,
- Minghui 86

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References(25)

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