Process Optimization and <i>in vitro</i> Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves

LIN Bao-mei; QIU Shan-lian; ZHENG Kai-bin; ZHANG Shuai; HONG Jia-min; ZHANG Shao-ping

doi:10.19303/j.issn.1008-0384.2019.05.012

Volume 34 Issue 5

Aug. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(5): 587-594

LIN Bao-mei, QIU Shan-lian, ZHENG Kai-bin, ZHANG Shuai, HONG Jia-min, ZHANG Shao-ping. Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012

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LIN Bao-mei, QIU Shan-lian, ZHENG Kai-bin, ZHANG Shuai, HONG Jia-min, ZHANG Shao-ping. Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves[J]. Fujian Journal of Agricultural Sciences, 2019, 34(5): 587-594. doi: 10.19303/j.issn.1008-0384.2019.05.012

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Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves

doi: 10.19303/j.issn.1008-0384.2019.05.012

Institute of Subtropical Agriculture, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China

Received Date: 2018-12-25
Rev Recd Date: 2019-03-12
Publish Date: 2019-05-28

Abstract

Abstract

Objective To optimize the polyphenol extraction from leaves of jaboticaba and determine the α-glucosidase inhibition by the extract. Method The ethanol extraction with ultrasound application was optimized by an orthogonal experiment. Effects of the power and time of ultrasonic application as well as the ethanol volume fraction, material-solvent ratio, and processing temperature on the total polyphenols extracted from the leaves were analyzed. The inhibition on α-glucosidase activity by the extract was determined in vitro by an enzyme kinetic study and uv-vis absorption spectrometry. Result The optimal extraction conditions included the use of 120 W ultrasonic wave for 10 min, 40% ethanol volume fraction, 1:160(g:mL) material-solvent ratio, and 40℃ processing temperature to achieve a polyphenol extraction rate of 33.84%. The α-glucosidase activity was significantly inhibited by the extract with a low IC₅₀ of 0.0031 mg·mL^-1, as compared to IC₅₀ of 8.110 8 mg·mL^-1 on acarbose. The kinetic study by the Lineweaver-Burk plot showed that the polyphenol extract was an anti-competitive inhibitor of α-glucosidase. Conclusion The polyphenol extract from jaboticaba leaves significantly inhibited α-glucosidase and could be explored for use as a hypoglycemic agent.
- Jaboticaba,
- polyphenol,
- α-glucosidase,
- orthogonal test

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References(20)

References

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