Isolation, Identification and Antibiotic Resistance of <i>Mannheimia Haemolytica</i> from Diseased Goats in Fujin

LIN Yu-sheng; JIANG Jin-xiu; ZHANG Jing-peng; YOU Wei; HU Qi-lin

doi:10.19303/j.issn.1008-0384.2019.03.011

Volume 34 Issue 3

Jun. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(3): 326-330

LIN Yu-sheng, JIANG Jin-xiu, ZHANG Jing-peng, YOU Wei, HU Qi-lin. Isolation, Identification and Antibiotic Resistance of Mannheimia Haemolytica from Diseased Goats in Fujin[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 326-330. doi: 10.19303/j.issn.1008-0384.2019.03.011

Citation:

LIN Yu-sheng, JIANG Jin-xiu, ZHANG Jing-peng, YOU Wei, HU Qi-lin. Isolation, Identification and Antibiotic Resistance of Mannheimia Haemolytica from Diseased Goats in Fujin[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 326-330. doi: 10.19303/j.issn.1008-0384.2019.03.011

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Isolation, Identification and Antibiotic Resistance of Mannheimia Haemolytica from Diseased Goats in Fujin

doi: 10.19303/j.issn.1008-0384.2019.03.011

Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2018-12-27
Rev Recd Date: 2019-02-26
Publish Date: 2019-03-28

Abstract

Abstract

Objective To identify and study the pathogen that caused the goat pneumonia in a farm in Fuqing, Fujian, and provide reference for the isolation, identification and detection of goat Mannheimia. Method Bacterial isolation and culture were conducted on the tissues taken by autopsy on the diseased goats. ORF virus, mycoplasma, ovipneumonia and mycoplasma cluster were performed by PCR. A microorganism was isolated and identified by 16S rRNA gene cloning and sequencing combined with PCR. Subsequently, sensitivities of the isolated strain toward various drugs were tested. Result The PCR detection showed an unique amplified band of the isolated strain that matched Mannheimia haemolytica. The 16S rRNA sequencing of the strain shared 97.1%-100.0% similarities with those of other strains of M. haemolytica published in GenBank which included an 100% homology with the D171 strain. Positively confirmed to be of the M. haemolytica family, the isolate was code-named FJ-FQ2018. The drug sensitivity test showed the isolate to be highly sensitive to flufenicol, enoxacin, cephalothiophene, cefazoline, ciprofloxacin, et al, moderately sensitive to ampicillin, and slightly sensitive to polymyxin B and lincomycin. Conclusion The pathogen of goat pneumonia isotaled from Fujian was a strain of M. haemolytica, and the drug sensitivities of the isolate was deemed varions toward 8 antibiotics.
- goat,
- Mannheimia haemolytica,
- isolation and identification,
- antibiotic resistance

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References(21)

References

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