Micromorphology of Leaves and Pollens of Wild Tea (<i>Camellia sinensis</i>) Germplasms from Shouning, Fujian

FAN Xiao-jing; YU Wen-tao; LIU Deng-yong; LU Ming-ji; ZHENG Jie; CHEN Xiao-lan; WEI Ming-xiu; LIN Yi; YE Nai-xing

doi:10.19303/j.issn.1008-0384.2019.03.007

Volume 34 Issue 3

Jun. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(3): 298-305

FAN Xiao-jing, YU Wen-tao, LIU Deng-yong, LU Ming-ji, ZHENG Jie, CHEN Xiao-lan, WEI Ming-xiu, LIN Yi, YE Nai-xing. Micromorphology of Leaves and Pollens of Wild Tea (Camellia sinensis) Germplasms from Shouning, Fujian[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 298-305. doi: 10.19303/j.issn.1008-0384.2019.03.007

Citation:

FAN Xiao-jing, YU Wen-tao, LIU Deng-yong, LU Ming-ji, ZHENG Jie, CHEN Xiao-lan, WEI Ming-xiu, LIN Yi, YE Nai-xing. Micromorphology of Leaves and Pollens of Wild Tea (Camellia sinensis) Germplasms from Shouning, Fujian[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 298-305. doi: 10.19303/j.issn.1008-0384.2019.03.007

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Micromorphology of Leaves and Pollens of Wild Tea (Camellia sinensis) Germplasms from Shouning, Fujian

doi: 10.19303/j.issn.1008-0384.2019.03.007

1.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province Fuzhou, Fujian 350002, China
2.
Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Inspection and Quarantine Technical Centre, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350001, China
3.
Tea Authority in Shouning County, Shouning, Fujian 355500, China

Received Date: 2018-09-12
Rev Recd Date: 2019-01-14
Publish Date: 2019-03-28

Abstract

Abstract

Objective Foliar and pollen micromorphology of wild tea germplasms from Shouning, Fujian was examined and recorded for references on the natural resource. Method Field emission scanning electron microscopy (FE-SEM) was used to observe the micro-structures of the leaves and pollens of 4 tea germplasms collected from Shouning. Twelve morphological characteristics on the leaves and 10 on the pollens were obtained for analysis. Result The leaf-surface stomates of these plants were oval in shape with glandular scales less densely scattered than the cultivated cultivars. The trichome lengths ranged between(280.75±107.65) μm and (616.95±132.52) μm with significant differences among the germplasms. The surface of trichome was smooth. The adaxial epidermal wax ornamentation was either flat, wrinkled or wavy in appearance. The pollens were all tricolporate of the N3P4C5 type with a size in between (427.69±153.94) μm² and (1205.07±237.80) μm², which was smaller than those of Fudingdabaicha tea. They had two kinds of polar view, suborbicular and trifid subtriangular; their equatorial views differed; their shapes, ellipsoid or subsphaeroidal; and, their ornamentations, verrucate. As compared to Fudingdabaicha, the 4 wild germplasms had certain distinct foliar and pollen micromorphological characteristics, which could serve for the differentiation of the species.
- Camellia sinensis,
- germplasm resource,
- Shouning,
- leaf,
- pollen,
- micromorphology

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References(30)

References

[1]	王平盛, 虞富莲.中国野生大茶树的地理分布、多样性及其利用价值[J].茶叶科学, 2002, 22(2): 105-108. doi: 10.3969/j.issn.1000-369X.2002.02.003 WANG P S, YU F L. The Geographic Distribution, Diversity and Utilization of Wild Tea Camellias in China[J]. Journal of Tea Science, 2002, 22(2): 105-108.(in Chinese) doi: 10.3969/j.issn.1000-369X.2002.02.003
[2]	汪劲武.两个姐妹科--茶科和猕猴桃科[J].生命世界, 1985(5):33-35. http://www.cnki.com.cn/Article/CJFDTotal-ZWZA198505027.htm WANG J W. Two sister families-Theaceae and Actinidiaceae Gilg & Werderm[J].Life World, 1985(5):33-35. (in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-ZWZA198505027.htm
[3]	郭元超, 姚信恩, 肖丽平, 等.仙墩山野生茶树考查报告[J].茶叶科学简报, 1984(1):6-11. http://www.cnki.com.cn/Article/CJFDTotal-CYKJ198401002.htm GUO Y C, YAO X E, XIAO L P, et al.Investigation report of wild tea trees in Xiandun Mountain[J]. Tea Science Briefing, 1984(1):6-11.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-CYKJ198401002.htm
[4]	郭元超, 叶乃兴, 肖丽平.太姥山野生茶树考查报告[J].茶叶科学简报, 1985(1):8-10. http://www.cnki.com.cn/Article/CJFDTotal-CYKJ198501002.htm GUO Y C, YE N X, XIAO L P. Taicha Mountain Wild Tea Tree Examination[J].ReportTea Science Briefing, 1984(1):6-11.(in Chinese) http://www.cnki.com.cn/Article/CJFDTotal-CYKJ198501002.htm
[5]	周玉璠, 吴洪新, 阮克錞, 等.宁德市陆续发现茶的野生资源[J].福建茶叶, 1994(3):8-10. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199400063882 ZHOU Y P, WU H X, RUAN K C, et al.Ningde City successively discovered the wild resources of tea[J]. Tea In Fujian, 1994(3):8-10.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199400063882
[6]	杨如兴, 陈芝芝, 张磊, 等.福建野生茶树种质资源保护与利用[J].茶叶学报, 2017, 58(3):96-101. doi: 10.3969/j.issn.1007-4872.2017.03.003 YANG R X, CHEN Z Z, ZHANG L, et al.Conservation and Utilization of Wild Tea Germplasm in Fujian[J]. Acta Tea Sinica, 2017, 58(3):96-101.(in Chinese) doi: 10.3969/j.issn.1007-4872.2017.03.003
[7]	SOSNOVSKY Y, NACHYCHKO V, PROKOPIV A, et al. Leaf architecture in Rhododendron subsection Rhododendron (Ericaceae) from the Alps and Carpathian Mountains: Taxonomic and evolutionary implications[J]. Flora, 2017, 230:26-38. doi: 10.1016/j.flora.2017.03.003
[8]	FIRETTI LEGGIERI F, LOHMANN L G, SEMIR J, et al. Using leaf anatomy to solve taxonomic problems within the Anemopaegma arvense species complex (Bignonieae, Bignoniaceae)[J]. Nordic Journal of Botany, 2015, 32(5):620-631. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=181eaaf801f72b97e40593de8aa1674b
[9]	汤雨岛, 廖晨阳, 周波.常绿杜鹃亚属麻花杜鹃亚组11种3变种植物叶片微形态特征及其分类学意义[J].西北植物学报, 2016, 36(8): 1600-1607. http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201608014 TANG Y D, LIAO C Y, ZHOU B. Leaf Micromorphological Characteristics and Taxonomic Significance of 11 Species, 3 Varieties in Subsect.Maculifera, Subgen.Hymenanthes(Ericaceae)[J]. Acta BotanicaBoreali-Occidentalia Sinica, 2016, 36(8): 1600-1607.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xbzwxb201608014
[10]	陈常颂, 彭艾, 钟秋生, 等. 34份茶树种质的花粉形态特征研究[J].福建农业学报, 2012, 27(11):1219-1226. doi: 10.3969/j.issn.1008-0384.2012.11.014 CHEN C S, PENG A, ZHONG Q S, et al.Morphological Studies on Pollen of Camellias for 34 Species (Camellia Sinensis)[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11):1219-1226.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.11.014
[11]	SUR G L, KEATING R, SNOW N, et al. Leaf Micromorphology Aids Taxonomic Delineation within the Hypervariable Genus Metrosideros (Myrtaceae) on O'ahu[J]. Pacific Science, 2018, 72(3):345-362. doi: 10.2984/72.3.6
[12]	FORTINI P, ANTONECCHIA G, MARZIO P D, et al. Role of micromorphological leaf traits and molecular data in taxonomy of three sympatric white oak species and their hybrids (Quercus L.)[J]. Giornale Botanico Italiano, 2013, 149(3):546-558. http://www.researchgate.net/publication/259397440_Role_of_micromorphological_leaf_traits_and_molecular_data_in_taxonomy_of_three_sympatric_white_oak_species_and_their_hybrids_(Quercus_L.)
[13]	胡蕙露, 杨景华, 陈慧, 等.若干科观赏植物花粉形态电镜观察与比较[J].安徽农业大学学报, 2001(3): 320-325. doi: 10.3969/j.issn.1672-352X.2001.03.024 HU H L, YANG J H, CHEN H, et al. The SEM Investigation on Structure and Shape of Ornamental Plant's Pollen in Several Families[J]. Journal of Anhui Agricultural University, 2001(3): 320-325.(in Chinese) doi: 10.3969/j.issn.1672-352X.2001.03.024
[14]	FURNESS C A, GREGORY T, RUDALL P J. Pollen Structure and Diversity in Liliales[J]. International Journal of Plant Sciences, 2015, 176(8):000-000. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=3e547f1e70000bd2447a5eb1d6bd198e
[15]	张永芳, 胡超琼, 杨勇, 等.柿属8种植物花粉形态观察[J].园艺学报, 2016(6): 1167-1174. http://d.old.wanfangdata.com.cn/Periodical/yyxb201606015 ZHANG Y F, HU C Y, YANG Y, et al. Pollen Morphology Observation of Eight Resources in Diospyros[J]. Acta Horticulturae Sinica, 2016(6): 1167-1174.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201606015
[16]	杨国一, 于文涛, 蔡春平, 等.茶树叶片扫描电镜样品制备方法的比较研究[J].江苏农业科学, 2018, 46(3): 95-98. http://d.old.wanfangdata.com.cn/Periodical/jsnykx201803024 YANG G Y, YU W T, CAI C P, et al. Comparative Study on Preparation Methods of Scanning Electron Microscope Samples of Tea Leaves[J]. Jiangsu Agricultural Sciences, 2018, 46(3): 95-98.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsnykx201803024
[17]	侯燕鸣, 胡剑江, 方蔷, 等.扫描电镜的不同含水量植物叶片样品的处理及观察方法研究[J].分析仪器, 2011(5): 45-48. doi: 10.3969/j.issn.1001-232X.2011.05.011 HOU Y M, HU J J, FANG Q, et al. Study on sample treatment methods of plant leaves with different water contents for SEM[J]. Analytical Instrumentation, 2011(5): 45-48.(in Chinese) doi: 10.3969/j.issn.1001-232X.2011.05.011
[18]	戴志聪, 杜道林, 司春灿, 等.用扫描仪及Image J软件精确测量叶片形态数量特征的方法[J].广西植物, 2009, 29(3):342-347. doi: 10.3969/j.issn.1000-3142.2009.03.013 DAI Z C, DU D L, SI C C, et al, A method to exactly measure the morphological quantity of leaf using Scanner and Image J Software[J].Guihaia, 2009, 29(3):342-347.(in Chinese) doi: 10.3969/j.issn.1000-3142.2009.03.013
[19]	谢微微, 于文涛, 杨国一, 等. 14个茶树品种的花粉微形态观察[J].南方农业学报, 2018, 49(9): 1698-1704. doi: 10.3969/j.issn.2095-1191.2018.09.03 XIE W W, YU W T, YANG G Y, Micromorphological observation on pollen of 14 cultivars of tea tree (Camellia sinensis) [J].Journal of Southern Agriculture, 2018, 49(9): 1698-1704.(in Chinese) doi: 10.3969/j.issn.2095-1191.2018.09.03
[20]	尹鹏, 刘威, 王广铭, 等.茶树芽叶茸毛及茶毫研究进展[J].河南农业, 2016(27): 44-46. doi: 10.3969/j.issn.1006-950X.2016.27.023 YIN P, LIU W, WANG G M, et al, Advances in research on tea tree bud leaf trichomes and teapubescence[J].Agriculture of Henan, 2016(27): 44-46.(in Chinese) doi: 10.3969/j.issn.1006-950X.2016.27.023
[21]	王开发, 王宪曾.孢粉学概论[M].北京:北京大学出版社, 1983. WANG K F, WANG X Z. Introduction to Playnology[M]. Beijing:Peking University Press, 1983. (in chinese)
[22]	王伏雄.中国植物花粉形态(第2版)[M].北京:科学出版社, 1995. WANG F X. Pollen Flora of China (Second Edition)[M]. Beijing:Science Press, 1995. (in chinese)
[23]	束际林.云南茶树种质资源花粉形态的研究[J].中国茶叶, 1990(4):12-13. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000001157224 SHU J L. Study on Pollen Morphology of Yunnan Tea Tree Germplasm Resources[J].China Tea, 1990(4):12-13.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000001157224
[24]	肖寒, 白洁.常绿杜鹃亚属云锦杜鹃亚组26种植物叶片微形态特征及其分类学意义[J].四川大学学报(自然科学版), 2013, 50(2). http://d.old.wanfangdata.com.cn/Periodical/scdxxb201302030 XIAO H, BAI J. Leaf micromorphological characteristics and taxonomic significance of twenty-six species of Subsect. Fortunea, subgen.Hymenanthe (Ericaceae)[J].Journal of Sichuan University(Natural Science Edition), 2013, 50(2). (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/scdxxb201302030
[25]	郭元超.茶树茸毛的形态特征与演化特点[J].茶叶科学简报, 1993(3): 1-4. http://www.cqvip.com/QK/91310X/199303/4001506630.html GUO Y C. Morphological characteristics and evolution characteristics of tea tree trichomes[J].ReportTea Science Briefing, 1993(3): 1-4.(in Chinese) http://www.cqvip.com/QK/91310X/199303/4001506630.html
[26]	杨国一, 于文涛, 郑晶, 等.乌龙茶种质叶片微形态特征的扫描电镜观察[J].南方农业学报, 2018, 49(10): 2020-2027. doi: 10.3969/j.issn.2095-1191.2018.10.18 YANG G Y, YU W T, ZHENG J, et al, Scanning electron microscopy observation on foliar micromorphology of Oolong tea germplasms[J].Journal of Southern Agriculture, 2018, 49(10): 2020-2027.(in Chinese) doi: 10.3969/j.issn.2095-1191.2018.10.18
[27]	邱勇娟, 李火坤, 陈佳, 等.扶绥野生茶树叶片结构与生产性状分析[J].南方农业学报, 2015, 46(2):286-292. doi: 10.3969/jissn.2095-1191.2015.2.286 QIU Y J, LI H K, CHEN J, et al, Leaf structure and production trait of wild tea(Camellia sinensis) from Fusui county[J].Journal of Southern Agriculture, 2015, 46(2):286-292.(in Chinese) doi: 10.3969/jissn.2095-1191.2015.2.286
[28]	袁弟顺, 叶乃兴, 刘金英, 等.白茶品种茸毛的生化特性[J].福建农林大学学报(自然科学版), 2010, 39(4):356-360. http://d.old.wanfangdata.com.cn/Periodical/fjnydxxb201004005 YUAN D S, YE N X, LIU J Y, et al, Biochemical characteristics of white tea pubescence[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition), 2010, 39(4):356-360.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/fjnydxxb201004005
[29]	孙崇波, 向林, 施季森, 等.兰科5属常见栽培品种花粉块形态的扫描电镜观察[J].园艺学报, 2010, 37(12): 1969-1974. http://d.old.wanfangdata.com.cn/Periodical/yyxb201012010 SUN C B, XIANG L, SHI J S, et al, Scanning Electron Microscope Observation on Pollinium Morphology of Five Most Common Cultivated Orchid Genera (Orchidaceae)[J].Acta Horticulturae Sinica, 2010, 37(12): 1969-1974.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/yyxb201012010
[30]	Al-HAKIMI A S, FARIDAH Q Z, ABKULWAHAB A S, et al. Pollen and seed morphology of, Barleria, L. (Barlerieae: Ruellioideae: Acanthaceae) of Yemen[J]. South African Journal of Botany, 2018, 116:185-191. doi: 10.1016/j.sajb.2018.03.010