Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress

LIAN Ling; XU Hui-bing; HE Wei; ZHU Yong-sheng; PAN Li-yan; WEI Yi-dong; ZHENG Yan-mei; LUO Xi; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2019.03.001

Volume 34 Issue 3

Jun. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(3): 255-263

LIAN Ling, XU Hui-bing, HE Wei, ZHU Yong-sheng, PAN Li-yan, WEI Yi-dong, ZHENG Yan-mei, LUO Xi, XIE Hua-an, ZHANG Jian-fu. Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 255-263. doi: 10.19303/j.issn.1008-0384.2019.03.001

Citation:

LIAN Ling, XU Hui-bing, HE Wei, ZHU Yong-sheng, PAN Li-yan, WEI Yi-dong, ZHENG Yan-mei, LUO Xi, XIE Hua-an, ZHANG Jian-fu. Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 255-263. doi: 10.19303/j.issn.1008-0384.2019.03.001

Citation:

LIAN Ling, XU Hui-bing, HE Wei, ZHU Yong-sheng, PAN Li-yan, WEI Yi-dong, ZHENG Yan-mei, LUO Xi, XIE Hua-an, ZHANG Jian-fu. Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 255-263. doi: 10.19303/j.issn.1008-0384.2019.03.001

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Expression of Antioxidant Enzyme Genes in Rice under PEG-simulated Drought-stress

doi: 10.19303/j.issn.1008-0384.2019.03.001

LIAN Ling^{1, 2
,},
XU Hui-bing^{1, 2},
HE Wei^{1, 2},
ZHU Yong-sheng^{1, 2},
PAN Li-yan^{1, 2},
WEI Yi-dong^{1, 2},
ZHENG Yan-mei^{1, 2},
LUO Xi^{1, 2},
XIE Hua-an^{1, 2
,
,},
ZHANG Jian-fu^{1, 2
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, China/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, Fujian 350003, China

Received Date: 2019-02-04
Rev Recd Date: 2019-02-26
Publish Date: 2019-03-28

Abstract

Abstract

Objective Expression of antioxidant enzyme genes of rice in response to drought-stress was studied. Method Simulated drought conditions using PEG6000 on Indica rice Hang 2 were used for the experimentation. The plants at 3-leaf stage were initially treated with 0% (CK), 18%, 20%, 22%, 24% or 26% PEG6000 to determine the appropriate concentration for the subsequent test. Under the selected PEG6000 treatment level, plant samples were collected at 0, 2, 4, 8, 12, 24, 48 and 72 h for analysis. The expressions of antioxidant enzyme genes (i.e., CATA, CATB and CATC), peroxidase genes (i.e., POX5.1 and POX1), superoxide dismutase genes (i.e., plastidic Cu/Zn-SOD and cytosolic Cu/Zn-SOD), ascorbate peroxidase gene (i.e., APX), and glutathione reductase gene (i.e., GR) of the rice plants were determined by qRT-PCR. Result Based on the phenotype and survival rate of the rice plants in the preliminary test, 22% PEG6000 was chosen for the simulation experiment. The results of qRT-PCR showed that all 9 genes were upregulated initially under the treatment but downregulated afterward. Most of the genes significantly upregulated 4 h after treatment showing a response of the genes to the stress. In particular, CATA exhibited a most significant change at 8 h which was 28 times of that at 0 h. Conclusion The expression of antioxidant enzyme genes significantly reacted to the PEG6000 treatment.
- rice,
- PEG6000,
- drought stress,
- antioxidant enzymes genes,
- expression analysis

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References(23)

References

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