Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

HE Hai-yan; QIU Hai-ping; CHAI Rong-yao; MAO Xue-qin; WANG Yan-li; SUN Guo-chang

doi:10.19303/j.issn.1008-0384.2019.02.012

Volume 34 Issue 2

Apr. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(2): 214-222

HE Hai-yan, QIU Hai-ping, CHAI Rong-yao, MAO Xue-qin, WANG Yan-li, SUN Guo-chang. Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 214-222. doi: 10.19303/j.issn.1008-0384.2019.02.012

Citation:

HE Hai-yan, QIU Hai-ping, CHAI Rong-yao, MAO Xue-qin, WANG Yan-li, SUN Guo-chang. Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 214-222. doi: 10.19303/j.issn.1008-0384.2019.02.012

Citation:

HE Hai-yan, QIU Hai-ping, CHAI Rong-yao, MAO Xue-qin, WANG Yan-li, SUN Guo-chang. Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 214-222. doi: 10.19303/j.issn.1008-0384.2019.02.012

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Distribution and Evaluation of 6 Blast Resistance Genes in Major Cultivated Rice Varieties in Zhejiang

doi: 10.19303/j.issn.1008-0384.2019.02.012

1.
Zhejiang Academy of Agricultural Sciences, Zhejiang Key Laboratory of Plant Pest Contro-Provincial and Ministerial Co-construction of National Key Laboratory Cultivation base, Hangzhou, Zhejiang 310021, China
2.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100080, China

Received Date: 2019-01-04
Rev Recd Date: 2019-01-31
Publish Date: 2019-02-28

Abstract

Abstract

Objective The distribution of 6 resistance genes in 40 cultivated rice varieties in Zhejiang (CRZ) and 6 physiological race identification (PRI) cultivars of Magnaporthe grisea as well as the blast resistance of the rice seedlings were studied. Method The distribution of 6 resistance genes, i.e., Pi1, Pi9/Piz, Pi2/Pizt, Pikh, Pikm and Pita, in the rice varieties was obtained using functional markers. The resistance to M. grisea of all 46 rice varieties was determined based on the 141 strains collected in the field from 2015 to 2017. Result Among the resistance genes, Pita had the highest distribution frequency at 47.83% followed by Pikh at 41.30%; Pi2/Pizt and Pikm, same at 34.78%; Pi9/Piz, low at 21.74%; and Pi1 found only in TTP, one of PRI cultivars. In all, there were 22 rice varieties that showed a resistance rate beyond 60%, and 2 varieties more than 80%. Conclusion The resistance genes carried by the 40 CRZ and the 6 PRI cultivars partially correlated with the resistance level exhibited by the cultivars.
- rice,
- rice leaf blast,
- molecular marker,
- resistance gene

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References(31)

References

[1]	郑永利, 施德, 姚晓明, 等.晚稻稻瘟病局部突发流行成因与治理对策探讨[J].浙江农业科学, 2015, 56(1):92-93. http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029 ZHENG Y L, SHI D, YAO X M, et al. Discussion on the causes of local outbreak of rice blast in late rice and its control countermeasures[J]. Journal of Zhejiang Agricultural Sciences, 2015, 56(1):92-93.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjnykx201501029
[2]	谢子正, 许渭根, 李仁忠, 等. 2014年浙江省水稻稻瘟病流行特点及原因分析[J].中国植保导刊, 2015(3):58-60. doi: 10.3969/j.issn.1672-6820.2015.03.014 XIE Z Z, XU W G, LI R Z, et al. Epidemiological characteristics and causes of rice blast in Zhejiang Province in 2014[J]. China Plant Protection, 2015(3):58-60.(in Chinese) doi: 10.3969/j.issn.1672-6820.2015.03.014
[3]	向聪, 雷东阳, 任西明, 等.水稻抗稻瘟病遗传育种研究进展[J].作物研究, 2017(5):547-552. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022 XIANG C, LEI D Y, REN X M, et al. Research progress in genetic and breeding of rice blast resistance[J].Crop Research, 2017(5):547-552.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201705022
[4]	ANDERSEN J R, LUBBERSTED T.Functional markers in plants[J].Trends in Plant Science, 2003, 8(11):554-560. doi: 10.1016/j.tplants.2003.09.010
[5]	谭令辞, 刘雄伦, 杨婷婷, 等.利用Pi9基因分子标记辅助选择培育抗稻瘟病水稻新品系[J].作物研究, 2015, 29(4):348-351. doi: 10.3969/j.issn.1001-5280.2015.04.04 TAN L C, LIU X L, YANG T T, et al. Breeding new rice line with Blast Resistant by molecular marker-assisted selection of the Pi9 Gene[J].Crop Research, 2015, 29(4):348-351.(in Chinese) doi: 10.3969/j.issn.1001-5280.2015.04.04
[6]	行璇, 刘雄伦, 陈海龙, 等.分子标记辅助选择Pi9基因改良R288的稻瘟病抗性[J].作物研究, 2016, 30(5):487-491. http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002 XING X, LIU X L, CHEN H L, et al. Improving blast resistance of rice restorer R288 by molecular marker-assisted selection of Pi9 Gene[J].Crop Research, 2016, 30(5):487-491.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowuyj201605002
[7]	梁毅, 杨婷婷, 谭令辞, 等.水稻广谱抗瘟基因Pigm紧密连锁分子标记开发及其育种应用[J].杂交水稻, 2013, 28(4):63-74. http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022 LIANG Y, YANG T T, TAN L C, et al. Development of the molecular marker tightly-linked with the broad-spectrum blast resistance gene Pigm and its breeding practice in Rice[J]. Hybrid Rice, 2013, 28(4): 63-74.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjsd201304022
[8]	杨丰宇, 李永聪, 刘雄伦, 等.分子标记辅助选择改良早籼稻1701的稻瘟病抗性[J].分子植物育种, 2017(6):2212-2217. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026 YANG F Y, LI YONGC, LIU X L, et al. Improving blast resistance of early indica rice 1701 by molecular marker-assisted selection for[J].Molecular Plant Breeding, 2017(6):2212-2217.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201706026
[9]	范方军, 王芳权, 刘永峰, 等. Pi-b、Pi-ta、Pikm和Pi54对水稻穗颈瘟的抗性评价[J].华北农学报, 2014, 29(3):221-226. http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040 FAN F J, WANG F Q, LIU Y F, et al. Evaluation of resistance to rice panicle blast with resistant genes Pi-b, Pi-ta, Pikm and Pi54[J].Acta Agriculturae Boreali-Sinica, 2014, 29(3):221-226.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnxb201403040
[10]	宋兆强, 刘艳, 王宝祥, 等.稻瘟病抗性基因Pi-ta、Pi-b、Pi54和Pi-km的育种利用价值评价[J].江苏农业学报, 2017, 33(5):968-974. doi: 10.3969/j.issn.1000-4440.2017.05.002 SONG Z Q, LIU Y, WANG B X, et al.[J]Application value of blast resistance genes Pi-ta, Pi-b, Pi54 and Pi-km in rice breeding[J]. Jiangsu Journal of Agricultural sciences, 2017, 33 (5): 968-974.(in Chinese) doi: 10.3969/j.issn.1000-4440.2017.05.002
[11]	International Rice Research Institute. Standard Evalution System for Rice[M]. 4th ed. Manila: IRRI, 1996:17-18.
[12]	HUA L X, WU J Z, CHEN C X, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast[J]. Theoretical & Applied Genetics, 2012, 125(5):1047-1055. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=5fab0b7df029d28060cc59d64d6ece46
[13]	刘开强, 伍豪, 颜群, 等.水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J].西南农业学报, 2016, 29(6):1241-1244. http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001 LIU K Q, WU H, YAN Q, et al. Development and application of specific molecular markers of blast resistance gene Pi1 in rice[J]. Southwest China Journal of Agricultural Sciences, 2016, 29 (6): 1241-1244.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/xnnyxb201606001
[14]	QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9/Piz encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice[J].Genetics, 2006, 172(3): 1901-1914. doi: 10.1534/genetics.105.044891
[15]	高利军, 高汉亮, 颜群, 等.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J].杂交水稻, 2010, 25(S1):294-298. http://d.old.wanfangdata.com.cn/Conference/7338573 GAO L J, GAO H L, YAN Q, et al. Establishment of markers for four blast genes and marker distribution in rice parents[J].Hybrid Rice, 2010, 25 (S1): 294-298.(in Chinese) http://d.old.wanfangdata.com.cn/Conference/7338573
[16]	ZHOU B, Qu S H, LIU G F, et al.The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions, 2006, 19(11): 1216-1228. doi: 10.1094/MPMI-19-1216
[17]	SHARMA T R, MADHAY M S, SINGH B K, et al. High-resolution mapping, cloning and molecular characterization of the Pi-k (h) gene of rice, which confers resistance to Magnaporthe grisea[J]. Molecular genetics and genomics: MGG, 2005, 274(6): 569-578. doi: 10.1007/s00438-005-0035-2
[18]	王军, 杨杰, 朱金燕, 等.稻瘟病抗病基因Pi-k^h功能标记的开发及江苏粳稻品种中Pi-k^h的变异[J].中国水稻科学, 2014, 28(2):141-14. doi: 10.3969/j.issn.1001-7216.2014.02.005 WANG J, YANG J, ZHU J Y, et al. Development of a functional marker for rice blast resistance gene Pi-k^h and natural variation atPi-k^h in Japonica rice in Jiangsu province[J].Chinese Journal of Rice Science, 2014, 28 (2): 141-147.(in Chinese) doi: 10.3969/j.issn.1001-7216.2014.02.005
[19]	ASHIKAWA I, HAYASHI N, YAMANE H, et al. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J]. Genetics, 2008, 180(4): 2267-2276. doi: 10.1534/genetics.108.095034
[20]	徐小金.稻瘟病抗性基因资源的挖掘及其育种利用[D].金华: 浙江师范大学, 2016. XU X J. Exploitation and breeding application of rice blast resistance gene resources[D]. Jinhua: Zhejiang Normal University, 2016.(in Chinese)
[21]	BRYAN G T, WU K S, FARRALL L, et al. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. The Plant Cell Online, 2000, 12(11): 2033-2045. doi: 10.1105/tpc.12.11.2033
[22]	JIA Y L, WANG Z H, SINGH P. Development of Dominant Rice Blast, Pi-ta, Resistance Gene Markers[J]. Crop Science, 2002, 42(6):2145-2149. doi: 10.2135/cropsci2002.2145
[23]	JIANG H C, FENG Y T, BAO L, et al. Improving blast resistance of Jin 23B and its hybrid rice by marker-assisted gene pyramiding[J]. Molecular Breeding, 2012, 30(4):1679-1688. doi: 10.1007/s11032-012-9751-6
[24]	陈红旗, 陈宗祥, 倪深, 等.利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性[J].中国水稻科学, 2008, 22(1):23-27. doi: 10.3321/j.issn:1001-7216.2008.01.004 CHEN H Q, CHEN Z X, NI S, et al. Pyramiding three genes with resistance to blast by marker-assisted to improve rice blast resistance of Jin23B[J]. Chinese Journal of Rice Science, 2008, 22(1): 23-27.(in Chinese) doi: 10.3321/j.issn:1001-7216.2008.01.004
[25]	柳武革, 王丰, 金素娟, 等.利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系稻瘟病抗性[J].作物学报, 2008, 34(7):1128-1136. http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004 LIU W G, WANG F, JIN S J, et al. Improvement of rice blast resistance of TGMS Line by pyramising of Pi-1 and Pi-2 through molecular marker-assisted selection[J].Acta Agromomica Sinica, 2008, 34(7): 1128-1136.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb200807004
[26]	DIVYA B, ROBIN S, RABINDRAN R, et al. Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43[J]. Euphytica, 2014, 200(1):79. doi: 10.1007/s10681-014-1182-5
[27]	于苗苗, 戴正元, 潘存红, 等.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应[J].作物学报, 2013, 39(11):1927-1934. http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002 YU M M, DAI Z Y, PAN C H, et al. Resistance spectrum difference between two broad-spectrum blast resistance genes, Pigmand Pi2, and their interaction effect on Pi1[J].Acta Agromomica Sinica, 2013, 39 (11): 1927-1934.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zuowxb201311002
[28]	邢鹏, 张幸, 李冬梅, 等.稻瘟病抗性基因在主要育种亲本中的分布研究[J].分子植物育种, 2015, 13(3):505-512. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004 XING P, ZHANG X, LI D M, et al. Research on the distribution of blast resistance genes in main rice breeding parents[J].Molecular Plant Breeding, 2015, 13(3): 505-512.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fzzwyz201503004
[29]	孙国昌, 杜新法, 陶荣祥, 等.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报, 1998(4):289-292. doi: 10.3321/j.issn:0412-0914.1998.04.001 SUN G C, DU X F, TAO R X, et al. Control tactics and prospect of rice blast research in 21th century[J]. Acta Phytopathologica Sinica, 1998 (4): 289-292.(in Chinese) doi: 10.3321/j.issn:0412-0914.1998.04.001
[30]	柴荣耀, 杜新法, 毛雪琴, 等.水稻不同类型品种上的稻瘟病菌群体小种的演变[J].浙江农业学报, 1999, 11(6):297-300. doi: 10.3969/j.issn.1004-1524.1999.06.005 CHAI H, DU X F, MAO X Q, et al. Race evolution of Magnaporthe grisea on different rice varieties[J].Acta Agriculturae Zhejianggensis, 1999, 11(6): 297-300.(in Chinese) doi: 10.3969/j.issn.1004-1524.1999.06.005
[31]	任鄄胜, 肖陪村, 陈勇, 等.水稻稻瘟病病菌研究进展[J].现代农业科学, 2008(1):19-23. http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019 REN J S, XIAO Y C, CHEN Y, et al. The research progress on rice blast fungus[J]. Modern Agricultural Science, 2008 (1): 19-23.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/ynnydxxb200101019