Pathogenicity of Serotype O<sub>78</sub> Avian Pathogenic <i>Escherichia coli</i> Isolated from Chicken and the Protective Effect of Butyric/Propionic Glycerides

LIU Yu-tao; LING Qing-biao; HUANG Bao-qin; WU Xue-min; WANG Long-bai; ZHOU Lun-jiang

doi:10.19303/j.issn.1008-0384.2019.02.011

Volume 34 Issue 2

Apr. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(2): 209-213

LIU Yu-tao, LING Qing-biao, HUANG Bao-qin, WU Xue-min, WANG Long-bai, ZHOU Lun-jiang. Pathogenicity of Serotype O78 Avian Pathogenic Escherichia coli Isolated from Chicken and the Protective Effect of Butyric/Propionic Glycerides[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 209-213. doi: 10.19303/j.issn.1008-0384.2019.02.011

Citation:

LIU Yu-tao, LING Qing-biao, HUANG Bao-qin, WU Xue-min, WANG Long-bai, ZHOU Lun-jiang. Pathogenicity of Serotype O₇₈ Avian Pathogenic Escherichia coli Isolated from Chicken and the Protective Effect of Butyric/Propionic Glycerides[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 209-213. doi: 10.19303/j.issn.1008-0384.2019.02.011

Citation:

PDF( 858 KB)

Pathogenicity of Serotype O₇₈ Avian Pathogenic Escherichia coli Isolated from Chicken and the Protective Effect of Butyric/Propionic Glycerides

doi: 10.19303/j.issn.1008-0384.2019.02.011

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
Hushi Station of Animal Husbandry and Veterinary Medicine of Xiuyu District, Putian, Fujian 351146, China
3.
Fujian Sunner Development Co. Ltd., Nanping, Fujian 354100, China

Received Date: 2018-10-09
Rev Recd Date: 2019-01-20
Publish Date: 2019-02-28

Abstract

Abstract

Objective Studying the pathogenicity of serotype O₇₈ Escherichia coli to laying hens with different inoculation methods and the protective effects of single glycerides could provide theoretical support and practical experience for green no-antibiotic chicken breeding. Method The virulence of serotype O₇₈ avian pathogenic Escherichia coli (APEC) strain isolated from a chicken farm in Fujian province was evaluated by oral, subcutaneous and intratracheal inoculation, and the protective effect of water-soluble liquid additive FraC34, composed of butyric and propionic acid monogly cerides, was evaluated in Hy-line laying hens. Result The results showed that:(1)With oral inoculation of 3×10⁹CFU bacteria per chicken, the morbidity was 100% for 12-days old Hy-line laying hens, but no disease detected for 22-days old ones with oral inoculation of 3×10⁹ or 6×10⁹CFU bacteria per chicken. When the 12-days old chicken were inoculated subcutaneously with 0.4×10⁹CFU bacteria per chicken, the rate of the sick was 70%, and 25% for 22-days old ones when inoculated intracheally with 1.5×10⁹ CFU bacteria per chicken. (2)Adding FraC34 in drinking water of laying hens could improve the intestinal tract and reduce the susceptibility to APEC in the early stage. The addition of 2.0-3.0 L·m^-3 FraC34 in drinking water could significantly reduce the morbidity and mortality rate for chickens infected with APEC by oral inoculation and mortality rate of which with intratracheal inner wall inoculation.The daily gain of chicken could be maintained when treated by 3.0 L·m^-3 FraC34. Conclusion The serotype O₇₈ APEC strain was pathogenic to young chicks and led to serious economic losses, but not susceptible for older chicken.FraC34 had protective effect and could maintain the production performance of the chicken infected with APEC, which could be applied instead of some antibiotics for efficient production of poultry.
- avian pathogenic Escherichia coli,
- butyric/propionic glycerides,
- Hy-line laying hens,
- protective effect

FullText(HTML)

References(13)

References

[1]	CROXEN M A, FINLAY B B. Molecular mechanisms of Escherichia coli pathogenicity[J]. Nature Reviews Microbiology, 2010, 8(1):26-38. doi: 10.1038/nrmicro2265
[2]	SMITH J L, FRATAMICO P M, GUNTHER N W. Extraintestinal pathogenic Escherichia coli[J]. Foodborne Pathogens & Disease, 2007, 4(2):134-163. http://d.old.wanfangdata.com.cn/Periodical/zgksszz200908012
[3]	JOHNSON T J, LOGUE C M, JOHNSON J R, et al. Associations between multidrug resistance, plasmid content, and virulence potential among extraintestinal pathogenic and commensal Escherichia coli from humans and poultry[J]. Foodborne Pathogens & Disease, 2012, 9(1):37-46. http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0225885774/
[4]	LI G, LATURNUS C, EWERS C, et al. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis[J]. Infection & Immunity, 2005, 73(5):2818-2827. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_1087346
[5]	WANG S, XIA Y, DAI J, et al. Novel roles for autotransporteradhesinAatA of avian pathogenic Escherichia coli: colonization during infection and cell aggregation[J]. Fems Immunology & Medical Microbiology, 2011, 63(3):328-338. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_PM22092559
[6]	刘玉涛, 周伦江, 王长兵, 等.闽北地区鸡致病性大肠杆菌的分离与血清型鉴定[J].福建农业学报, 2000, 15(3):51-53. doi: 10.3969/j.issn.1008-0384.2000.03.009 LIU Y T, ZHOU L J, WANG C B, etal. Isolation and serotype identification of chicken Escherichia coli in northern Fujian[J]. Fujian Journal ofAgricultural Sciences, 2000, 15(3): 51-53. (in Chinese) doi: 10.3969/j.issn.1008-0384.2000.03.009
[7]	田胜, 王晓云, 刘宝胜.饲料中滥用抗生素的危害及应对措施[J].中国饲料添加剂, 2010(7):5-9. http://www.cqvip.com/Main/Detail.aspx?id=34646754 TIAN S, WANG X Y, LIU B S. Harm and response measures of abusing antibiotics in feedstuff[J]. China Feed Additive, 2010(7):5-9. (in Chinese) http://www.cqvip.com/Main/Detail.aspx?id=34646754
[8]	李宁, 穆淑琴, 闫峻, 等.抗生素替代物在畜牧生产中的应用[J].饲料研究, 2017(17):1-5. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=slyj201717001 LI N, MU S Q, YAN J, et al. Application of antibiotic substitute in animal husbandry[J]. Feed Research, 2017(17):1-5. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=slyj201717001
[9]	李红, 孙东弦, 陈瑞旭, 等.添加剂对面包品质的影响及抗老化作用的研究[J].食品工业, 2016(5):5-8. doi: 10.3969/j.issn.1672-5026.2016.05.002 LI H, SUN D X, CHEN R X, et al. Studies on the effect of additives on bread quality and its anti-aging properties[J]. The Food Industry, 2016(5):5-8. (in Chinese) doi: 10.3969/j.issn.1672-5026.2016.05.002
[10]	NAMKUNG H, YU H, GONG J, et al. Antimicrobial activity of butyrate glycerides toward Salmonella Typhimurium and Clostridium perfringens[J]. Poultry Science, 2011, 90(10):2217-2222. doi: 10.3382/ps.2011-01498
[11]	张希, 杨明, 宋飞, 等.脂肪酸及其衍生物的抑菌活性[J].浙江大学学报(农业与生命科学版), 2013, 39(2):155-160. http://d.old.wanfangdata.com.cn/Periodical/zjdxxb-nyysm201302007 ZHANG X, YANG M, SONG F, et al. Antimicrobial activity of selected fatty acids and their derivatives[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2013, 39(2):155-160. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjdxxb-nyysm201302007
[12]	SAIF Y M.禽病学:第十二版[M].北京:中国农业出版社, 2012:811-863 SAIF Y M.Poultry disease:12ed[M]. Beijing: China Agriculture Press, 2012:811-863. (in Chinese)
[13]	王秋峰, 王振, 刘振伟. AC34单丁/丙酸甘油酯在饲料中的应用浅析[J].中国饲料添加剂, 2014(11):33-35. http://www.cqvip.com/QK/89585X/201411/663015094.html WANG Q F, WANG Z, LIU Z W. Application of AC34 single butyric (propionic) acid glycerides in feed.[J]. China Feed Additive, 2014(11):33-35. (in Chinese) http://www.cqvip.com/QK/89585X/201411/663015094.html