Correlation Between <i>GL</i>6 Expression Driven by Varied Promoters and Development of Epidermal Hairs on Leaves of Rice Plant

ZHU Yong-sheng; XIAO Kai-zhuan; WANG Fu-xiang; LIAN Ling; HE Wei; XU Hui-bin; WEI Yi-dong; CHEN Li-ping; JIANG Jia-huan; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2019.02.001

Volume 34 Issue 2

Apr. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(2): 139-145

ZHU Yong-sheng, XIAO Kai-zhuan, WANG Fu-xiang, LIAN Ling, HE Wei, XU Hui-bin, WEI Yi-dong, CHEN Li-ping, JIANG Jia-huan, XIE Hua-an, ZHANG Jian-fu. Correlation Between GL6 Expression Driven by Varied Promoters and Development of Epidermal Hairs on Leaves of Rice Plant[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 139-145. doi: 10.19303/j.issn.1008-0384.2019.02.001

Citation:

ZHU Yong-sheng, XIAO Kai-zhuan, WANG Fu-xiang, LIAN Ling, HE Wei, XU Hui-bin, WEI Yi-dong, CHEN Li-ping, JIANG Jia-huan, XIE Hua-an, ZHANG Jian-fu. Correlation Between GL6 Expression Driven by Varied Promoters and Development of Epidermal Hairs on Leaves of Rice Plant[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 139-145. doi: 10.19303/j.issn.1008-0384.2019.02.001

Citation:

ZHU Yong-sheng, XIAO Kai-zhuan, WANG Fu-xiang, LIAN Ling, HE Wei, XU Hui-bin, WEI Yi-dong, CHEN Li-ping, JIANG Jia-huan, XIE Hua-an, ZHANG Jian-fu. Correlation Between GL6 Expression Driven by Varied Promoters and Development of Epidermal Hairs on Leaves of Rice Plant[J]. Fujian Journal of Agricultural Sciences, 2019, 34(2): 139-145. doi: 10.19303/j.issn.1008-0384.2019.02.001

PDF( 1849 KB)

Correlation Between GL6 Expression Driven by Varied Promoters and Development of Epidermal Hairs on Leaves of Rice Plant

doi: 10.19303/j.issn.1008-0384.2019.02.001

ZHU Yong-sheng^{1, 2, 3
,},
XIAO Kai-zhuan^{1, 2, 3},
WANG Fu-xiang^{1, 2, 3},
LIAN Ling^{1, 2, 3},
HE Wei^{1, 2, 3},
XU Hui-bin^{1, 2, 3},
WEI Yi-dong^{1, 2, 3},
CHEN Li-ping^{1, 2, 3},
JIANG Jia-huan^{1, 2, 3},
XIE Hua-an^{1, 2, 3},
ZHANG Jian-fu^{1, 2, 3
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China
2.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
3.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, China/Fuzhou Branch, National Rice Improvement Center of China/Incubator of National Key Laboratory of Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China

Received Date: 2018-12-20
Rev Recd Date: 2019-01-05
Publish Date: 2019-02-28

Abstract

Abstract

Objective Being one of the important agronomic traits that reflect the yield and physiological functions of a rice plant, the growth and photosynthesis of the fine hairs on the surface of rice leaves were studied. Method The sequences of GL6 gene promoters relating to leaf epidermal hair development were obtained and cloned from different rice varieties. That of Mutant 75-1-127 showing the characteristic epidermal hair development was compared with those of the wild type rice lacking it. The CDS sequence of the mutant gene was cloned. Then, the maize Ubiquitin and cauliflower mosaic virus CaMV35S were used as promoter-driven overexpression vectors to transform the wild-type Japonica rice, Kitaake, mediated by Agrobacterium. Result There were significant differences on the sequences in regions of the genes regulated by the promoters and cloned from different cultivars. The leaves of transgenic rice plants with GL6 driven by maize Ubiquitin promoter had significant hairy phenotype of the typical velvet appearance, but not those involved CaMV35S as a promoter. Conclusion It was clearly demonstrated that the promoters affected the expression of the target gene GL6 and that the epidermal hair development on leaves of Mutant 75-1-127 was derived by the promoter with a unique sequence.
- rice,
- promoter,
- gene expression,
- leaf epidermal hair development

FullText(HTML)

References(24)

References

[1]	INOMURA K, BRAGG J, FOLLOWS M J. A quantitative analysis of the direct and indirect costs of nitrogen fixation:A model based on Azoto-bactervinelandii[J]. ISME J, 2017, 11:166. doi: 10.1038/ismej.2016.97
[2]	LAUTER D J, MUNNS D N. Water loss via the glandular trichomes of chickpea (Cicer arietinum L.)[J]. J Exp Bot, 1986, 37:640-649. doi: 10.1093/jxb/37.5.640
[3]	YAN A, PAN J, AN L, et al. The responses of trichome mutants to enhanced ultraviolet-B radiation in Arabidopsis thaliana[J]. J PhotochemPhotobiol B Biol, 2012, 113:29-35. doi: 10.1016/j.jphotobiol.2012.04.011
[4]	TATTINI M, GRAVANO E, PINELLI P, et al. Flavonoids accumulate in leaves and glandular trichomes of Phillyrealatifolia exposed to excess solar radiation[J]. New Phytol, 2000, 148:69-77. doi: 10.1046/j.1469-8137.2000.00743.x
[5]	尚宏芹, 刘建萍.干旱胁迫下不同茸毛性状辣椒植株抗旱性比较[J].核农学报, 2010, 24(4):835-839. http://d.old.wanfangdata.com.cn/Periodical/hnxb201004031 SHANG H Q, LIU J P. Comparison of drought resistance of pepper with different hairiness character under drought stress[J].Journal of Nuclear Agricultural Sciences, 2010, 24(4):835-839.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hnxb201004031
[6]	CASTILLO-LÓPEZ J L, CANO-SANTANA Z, OYAMA K. Preferences and survival of Lophoceramicapyrrha, a shelter builder gregarious noctuid, in two host plants[J].Dugesiana, 2010, 17:229-236. https://www.cabdirect.org/cabdirect/abstract/20113106790
[7]	DALIN P, GREN J, BJRKMAN C, et al. Chapter 4:Leaf trichome formation and plant resistance to herbivory.Induced Plant Resistance to Herbivory[M]. Netherlands:Springer, 2008:89-105.
[8]	MCGINNIS A J, KASTING R. Dietary cellulose:Effect on food consumption and growth of a grasshopper[J]. Ca J Zool, 2011, 45:165-167. http://cn.bing.com/academic/profile?id=4ccd573840db1ff1b9cc035f3f792930&encoded=0&v=paper_preview&mkt=zh-cn
[9]	HANDLEY R, EKBOM B, AGREN J. Variation in trichome density and resistance against a specialist insect herbivore in natural populations of Arabidopsis thaliana[J]. EcolEntomol, 2005, 30:284-292. doi: 10.1111/j.0307-6946.2005.00699.x
[10]	AN L, ZHOU Z, SU S, et al. GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome branching through gibberellic acid signaling in Arabidopsis[J]. Plant Cell Physiol, 2012, 53:457. doi: 10.1093/pcp/pcr192
[11]	KHOSLA A, PAPER J M, BOEHLER A P, et al. HD-Zip proteins GL2 and HDG11 have redundant functions in Arabidopsis trichomes and GL2 activates a positive feedback loop via MYB23[J]. Plant Cell, 2014, 26:2184-2200. doi: 10.1105/tpc.113.120360
[12]	MOROHASHI K, ZHAO M, YANG M, et al. Participation of the ArabidopsisbHLH factor GL3 in trichome initiation regulatory events[J]. Plant Physiol, 2007, 145:736-746. doi: 10.1104/pp.107.104521
[13]	BERNHARDT C, LEE M M, GONZALEZ, et al.ThebHLH genes GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root[J]. Development, 2003, 130:6431-6439. doi: 10.1242/dev.00880
[14]	CHOPRA D, WOLFF H, SPAN J, et al. Analysis of TTG1 function in Arabisalpina[J]. BMC Plant Biol, 2014, 14:16. doi: 10.1186/1471-2229-14-16
[15]	LARKIN J C, OPPENHEIMER D G, MARKS M D, et al. The GL1 gene and the trichome developmental pathway in Arabidopsis thaliana[J]. Results Probl Cell Differ, 1994, 20:259-275. doi: 10.1007/978-3-540-48037-2
[16]	SZYMANSKI D B, JILK R A, POLLOCK S M, et al. Control of GL2 expression in Arabidopsis leaves and trichomes[J]. Development, 1998, 125:1161-1171. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=6e5bae151798cddf42ed4ac8fb9edda9
[17]	SUN W Q, GAO D W, XIONG Y, et al.Hairy Leaf 6, an AP2/ERF Transcription Factor, Interacts with OsWOX3B and Regulates Trichome Formation in Rice[J].Molecular Plant, 2017, 10(11):1417-1433. doi: 10.1016/j.molp.2017.09.015
[18]	LI J J, YUAN Y D, LU Z F, et al. Glabrous Rice 1, encoding a homeodomainprotein, regulates trichome development in rice[J].Rice, 2012, 5(32):1-10. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883694/
[19]	QIN B, TANG D, HUANG J, et al. Rice OsGL1-1 is involved in leaf cuticular wax and cuticle membrane[J]. Mol Plant, 2011, 4:985-995. doi: 10.1093/mp/ssr028
[20]	曾跃辉, 朱永生, 连玲, 等.水稻茸毛基因GL6的遗传学分析与精细定位[J].科学通报, 2013, 58(1):1027-1035. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kxtb201311010 ZENG Y H, ZHU Y S, LIAN L, et al.Genetic analysis and fine mapping of the pubescence gene GL6 in rice (Oryzae sativa L.)[J].Chinese Science Bulletin, 2013, 58(1):1-8.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kxtb201311010
[21]	ZHENG K, TIAN H, HU Q, et al. Ectopic expression of R3MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation[J]. Sci Rep, 2016, 6:19254. doi: 10.1038/srep19254
[22]	KASUGA M, LIU Q, MIURA S, et al. Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J]. Nat biotechnol, 1999, 17(3):287-291 doi: 10.1038/7036
[23]	乔龙飞, 于延冲.LEAFY启动子中W-box对LEAFY表达及拟南芥开花的影响[J].植物生理学报, 2017, 53(4):713-720. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwslxtx201704024 QIAO L F, YU Y C. Effect of W-box in LEAFY promoter on LEAFY expression and Arabidopsis flowering[J]. Plant Physiology Journal, 2017, 53(4):713-720.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwslxtx201704024
[24]	陈兆骞, 陈熙, 张炜, 不同启动子在水稻悬浮细胞中诱导外源基因的表达, 江苏农业学报[J], 2014, 30(6):1208-1215. doi: 10.3969/j.issn.1000-4440.2014.06.004 CHEN Z Q, CHEN X, ZHANG W, et al. Promoter-induced expression of exogenous gene in rice suspension cell lines[J]. Jiangsu Journal of Agricultural Sciences, 2014, 30(6):1208-1215.(in Chinese) doi: 10.3969/j.issn.1000-4440.2014.06.004