Green Fluorescent Protein Genetic Marker of <i>Fusarium oxysporum</i> F-02 of Stem Rot Disease on <i>Cymbidium ensifolium</i>

YAO Jin-ai; ZHANG Hong; HUANG Peng; CHEN Feng; YU De-yi

doi:10.19303/j.issn.1008-0384.2019.01.011

Volume 34 Issue 1

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(1): 70-75

YAO Jin-ai, ZHANG Hong, HUANG Peng, CHEN Feng, YU De-yi. Green Fluorescent Protein Genetic Marker of Fusarium oxysporum F-02 of Stem Rot Disease on Cymbidium ensifolium[J]. Fujian Journal of Agricultural Sciences, 2019, 34(1): 70-75. doi: 10.19303/j.issn.1008-0384.2019.01.011

Citation:

YAO Jin-ai, ZHANG Hong, HUANG Peng, CHEN Feng, YU De-yi. Green Fluorescent Protein Genetic Marker of Fusarium oxysporum F-02 of Stem Rot Disease on Cymbidium ensifolium[J]. Fujian Journal of Agricultural Sciences, 2019, 34(1): 70-75. doi: 10.19303/j.issn.1008-0384.2019.01.011

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Green Fluorescent Protein Genetic Marker of Fusarium oxysporum F-02 of Stem Rot Disease on Cymbidium ensifolium

doi: 10.19303/j.issn.1008-0384.2019.01.011

YAO Jin-ai^{1, 2
,},
ZHANG Hong³,
HUANG Peng¹,
CHEN Feng¹,
YU De-yi^{1, 2
,
,}

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou, Fujian 350003, China
3.
Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China

Received Date: 2018-10-19
Rev Recd Date: 2018-11-22
Publish Date: 2019-01-28

Abstract

Abstract

Objective Green fluorescent protein(GFP) genetic marker of Fusarium oxysporum F-02 of stem rot disease on Cymbidium ensifolium were conducted in this study in order to observe the inflection of F. oxysporum on C. ensifolium plants, confirm the process of the pathogen, and provide powerful technic for further studies on F. oxysporum. Method GFP was transformed into F.oxysporum strain F-02 mediated by Agrobacterium, and the genetic stability of GFP in transformants was investigated. Result GFP gene can be successfully introduced into F.oxysporum and stable green fluorescence can be produced in the hypha and spores under the excitation of blue light source.After 10 successive transfers, the progenies grew well with stable green fluorescence and similar pathogenicity as parental transfrormant. Conclusion GFP gene was inherited stablely in C.ensifolinm and no effect on pathogenicity in F-02 strain.
- Cymbidium ensifolium,
- stem rot,
- Fusarium oxysporum,
- green fluorescent protein,
- genetic marker

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References(20)

References

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