cDNA Cloning, Tissue Expression Analysis of <i>MITF</i> Gene in Liancheng White Ducks

XIN Qing-wu; LIU Yi-zhan; ZHU Zhi-ming; LI Li; ZHANG Lin-li; MIAO Zhong-wei; ZHENG Nen-zhu

doi:10.19303/j.issn.1008-0384.2018.12.011

Volume 33 Issue 12

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(12): 1292-1300

XIN Qing-wu, LIU Yi-zhan, ZHU Zhi-ming, LI Li, ZHANG Lin-li, MIAO Zhong-wei, ZHENG Nen-zhu. cDNA Cloning, Tissue Expression Analysis of MITF Gene in Liancheng White Ducks[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1292-1300. doi: 10.19303/j.issn.1008-0384.2018.12.011

Citation:

XIN Qing-wu, LIU Yi-zhan, ZHU Zhi-ming, LI Li, ZHANG Lin-li, MIAO Zhong-wei, ZHENG Nen-zhu. cDNA Cloning, Tissue Expression Analysis of MITF Gene in Liancheng White Ducks[J]. Fujian Journal of Agricultural Sciences, 2018, 33(12): 1292-1300. doi: 10.19303/j.issn.1008-0384.2018.12.011

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cDNA Cloning, Tissue Expression Analysis of MITF Gene in Liancheng White Ducks

doi: 10.19303/j.issn.1008-0384.2018.12.011

1.
Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2018-10-10
Rev Recd Date: 2018-11-19
Publish Date: 2018-12-28

Abstract

Abstract

The cloning, tissue expression and structural analysis of Microphthalmia-associated transcription factor (MITF) gene were conducted in Liancheng white duck, which would provide a theoretical basis for the further research of melanin deposition.The MITF gene from the skin of female ducks was cloned through nested-PCR, and the mRNA expression detected in tissues by qRT-PCR. It was shown that the MITF cDNA was 1 323 bp in length (GenBank No. MG516570) with a complete CDS region (34-1 323 bp) encoding 429 amino acids. The amino acid sequence of MITF was 100% homologous to that of mallard and more than 95% to that of Gallus gallus, but farther from sheep and other mammals. The predicted secondary structure of the protein was mainly random coil and α-helix, while the circular tertiary structure contained two separate helices to form the helix-loop-helix-chain structure. The qRT-PCR results showed that the mRNA expression of MITF was significantly higher in the skin than the other organs (P < 0.01) with the relative expressions in the order of skin > kidney > gizzard > liver > muscle. It appeared that the expression of MITF gene was tissue-specific in Liancheng white ducks. The information would aid in the utilization of MITF gene for studies on the melanin deposition and trait selection of the duck.
- Liancheng white duck,
- microphthalmia-associated transcription factor,
- sequence analysis,
- gene expression

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References(31)

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