Expression Stabilities of Three Housekeeping Genes of <i>Anoectochilus roxburghii</i>

LIN Jiang-bo; WANG Wei-ying; ZOU Hui; DAI Yi-min

doi:10.19303/j.issn.1008-0384.2018.11.001

Volume 33 Issue 11

Mar. 2019

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LIN Jiang-bo, WANG Wei-ying, ZOU Hui, DAI Yi-min. Expression Stabilities of Three Housekeeping Genes of Anoectochilus roxburghii[J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1125-1129. doi: 10.19303/j.issn.1008-0384.2018.11.001

Citation:

LIN Jiang-bo, WANG Wei-ying, ZOU Hui, DAI Yi-min. Expression Stabilities of Three Housekeeping Genes of Anoectochilus roxburghii[J]. Fujian Journal of Agricultural Sciences, 2018, 33(11): 1125-1129. doi: 10.19303/j.issn.1008-0384.2018.11.001

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Expression Stabilities of Three Housekeeping Genes of Anoectochilus roxburghii

doi: 10.19303/j.issn.1008-0384.2018.11.001

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China

Received Date: 2018-09-30
Rev Recd Date: 2018-11-02
Publish Date: 2018-11-01

Abstract

Abstract

Three unigenes of GAPDH (GenBank accession number:MH899010), Actin (GenBank accession number:MH899011) and α-tubulin (GenBank accession number:MH899012) were selected from Anoectochilus roxburghii transcriptome data. Their expression stabilities under various temperatures at different plant growth stages were studied. The results indicated that the expressions of GAPDH and α-tubulin genes varied under the test conditions, but that of Actin in the A. roxburghii stems and leaves was stable and consistent. It appeared that the gene of Actin would be a plausible candidate to be used as a reference in analyzing the expression of functional genes under varied temperatures during different growth stages.
- Anoectochilus roxburghii,
- GAPDH,
- Actin,
- α-tubulin

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References(26)

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