Optimized Extraction of Functional Ingredients from <i>Callicarpa nudiflora</i> Hook.et Arn.by Box-Behnken Design

HUANG Mei; YU Fu-lai; TAN Xiang-jie; CHEN Zhen-xia; PANG Yu-xin; HU Xuan; LI Wei

doi:10.19303/j.issn.1008-0384.2018.10.019

Volume 33 Issue 10

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(10): 1119-1124

HUANG Mei, YU Fu-lai, TAN Xiang-jie, CHEN Zhen-xia, PANG Yu-xin, HU Xuan, LI Wei. Optimized Extraction of Functional Ingredients from Callicarpa nudiflora Hook.et Arn.by Box-Behnken Design[J]. Fujian Journal of Agricultural Sciences, 2018, 33(10): 1119-1124. doi: 10.19303/j.issn.1008-0384.2018.10.019

Citation:

HUANG Mei, YU Fu-lai, TAN Xiang-jie, CHEN Zhen-xia, PANG Yu-xin, HU Xuan, LI Wei. Optimized Extraction of Functional Ingredients from Callicarpa nudiflora Hook.et Arn.by Box-Behnken Design[J]. Fujian Journal of Agricultural Sciences, 2018, 33(10): 1119-1124. doi: 10.19303/j.issn.1008-0384.2018.10.019

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Optimized Extraction of Functional Ingredients from Callicarpa nudiflora Hook.et Arn.by Box-Behnken Design

doi: 10.19303/j.issn.1008-0384.2018.10.019

HUANG Mei^1
,,
YU Fu-lai^{1
,
,},
TAN Xiang-jie¹,
CHEN Zhen-xia¹,
PANG Yu-xin^{1, 2
,
,},
HU Xuan¹,
LI Wei³

1.
Tropical crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Haikou, Hainan 571101, China
2.
School of Chinese Materia Resources Guangdong Pharmaceutical University, Yunfu, Guangdong 527500, China
3.
Hainan Jiuzhitang Pharmaceutical Co., Ltd., Haikou, Hainan 570311, China

Received Date: 2018-06-11
Rev Recd Date: 2018-09-14
Publish Date: 2018-10-01

Abstract

Abstract

To optimize the extraction of functional ingredients from the leaves of Callicarpa nudiflora Hook. et Arn., an experiment based on the Box-Behnken design was conducted. The extraction rate and contents of functional ingredients including flavonoids, phenolic acids, and saponins in the resulting extracts were weighed against the method, duration, cycle, material to solvent ratio, and ethanol concentration of the process. The weighted value for evaluation was calculated by the formula of(dry weight extract 0.7+ combined weight of functional ingredients 0.3). The optimized conditions included the application of ultrasound for 42 min, a material:solvent ratio of 1:17, the use of 62% ethanol for solvent, and two extraction cycles to arrive at a weighted value of 119.27, which was close to the predicted value of 120.47. The established extraction process was simple and applicable with desirable reproducibility and predictability.
- Callicarpa nudiflora Hook.et Arn.,
- functional ingredients,
- dry extract yield,
- response surface design,
- process optimization

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References(15)

References

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