Construction and Genetic Background Recovery of Blast-resistant Near-isogenic Lines from Japonica Variety, Yunyin

ZHU Yong-sheng; HE Wei; LIAN Ling; XIE Hong-guang; WEI Yi-dong; XU Hui-bin; ZHENG Yan-mei; WANG Ying-heng; CHEN Li-ping; CAI Qiu-hua; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2018.09.012

Volume 33 Issue 9

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(9): 957-962

ZHU Yong-sheng, HE Wei, LIAN Ling, XIE Hong-guang, WEI Yi-dong, XU Hui-bin, ZHENG Yan-mei, WANG Ying-heng, CHEN Li-ping, CAI Qiu-hua, XIE Hua-an, ZHANG Jian-fu. Construction and Genetic Background Recovery of Blast-resistant Near-isogenic Lines from Japonica Variety, Yunyin[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 957-962. doi: 10.19303/j.issn.1008-0384.2018.09.012

Citation:

ZHU Yong-sheng, HE Wei, LIAN Ling, XIE Hong-guang, WEI Yi-dong, XU Hui-bin, ZHENG Yan-mei, WANG Ying-heng, CHEN Li-ping, CAI Qiu-hua, XIE Hua-an, ZHANG Jian-fu. Construction and Genetic Background Recovery of Blast-resistant Near-isogenic Lines from Japonica Variety, Yunyin[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 957-962. doi: 10.19303/j.issn.1008-0384.2018.09.012

Citation:

ZHU Yong-sheng, HE Wei, LIAN Ling, XIE Hong-guang, WEI Yi-dong, XU Hui-bin, ZHENG Yan-mei, WANG Ying-heng, CHEN Li-ping, CAI Qiu-hua, XIE Hua-an, ZHANG Jian-fu. Construction and Genetic Background Recovery of Blast-resistant Near-isogenic Lines from Japonica Variety, Yunyin[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 957-962. doi: 10.19303/j.issn.1008-0384.2018.09.012

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Construction and Genetic Background Recovery of Blast-resistant Near-isogenic Lines from Japonica Variety, Yunyin

doi: 10.19303/j.issn.1008-0384.2018.09.012

ZHU Yong-sheng^{1, 2
,},
HE Wei^{1, 2},
LIAN Ling^{1, 2},
XIE Hong-guang^{1, 2},
WEI Yi-dong^{1, 2},
XU Hui-bin^{1, 2},
ZHENG Yan-mei^{1, 2},
WANG Ying-heng^{1, 2},
CHEN Li-ping^{1, 2},
CAI Qiu-hua^{1, 2},
XIE Hua-an^{1, 2
,
,},
ZHANG Jian-fu^{1, 2
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China
2.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture, P. R China/National Engineering Laboratory of Rice/Incubator of Key Laboratory of Germplasm Enhancement and Molecular Breeding Between Fujian and Ministry of Sciences and Technology/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China

Received Date: 2018-03-12
Rev Recd Date: 2018-08-14
Publish Date: 2018-09-01

Abstract

Abstract

Construction of near-isogenic lines(NILs) is an effective mean for analyzing blast-resistance genes as well as genetic research in general. This study applied the broad-spectrum blast-resistant japonica variety, Yunyin, as the donor parent and the blast-susceptible variety, LTH, as the recurrent parent. Combining the molecular markers assisted selection and artificial inoculation, 7 blast-resistant NILs were bred with LTH for the genetic background analysis after 4 generations of backcrossing and 8 generations of self-crossing.184 pairs of SSR markers evenly distributed on 12 chromosomes of the rice were selected to evaluate the genetic background of the NILs. The results showed that 72 pairs of SSR primers to be polymorphic with a frequency of 39.1%. The polymorphisms between 7 NILs and LTH were analyzed by markers among the parents. The background recovery rates of W15 and W17 were 97.61%, that of W18, 97.22%, and those of other 4 lines, greater than 98%. It appeared that the construction of NILs carrying Yunyin blast-resistance gene with LTH as background were successful, and that all phenotypic indices of the NILs recovered well in this study.
- rice,
- rice blast-resistance,
- near-isogenic lines,
- genetic background

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References(17)

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