Evaluation on 20 <i>Lentinula edodes</i> Strains Using ISSR and F-MSAP Markers

XIAO Dong-lai; ZHANG Di; LIN Yan-quan; YANG Jing

doi:10.19303/j.issn.1008-0384.2018.08.005

Volume 33 Issue 8

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(8): 794-798

XIAO Dong-lai, ZHANG Di, LIN Yan-quan, YANG Jing. Evaluation on 20 Lentinula edodes Strains Using ISSR and F-MSAP Markers[J]. Fujian Journal of Agricultural Sciences, 2018, 33(8): 794-798. doi: 10.19303/j.issn.1008-0384.2018.08.005

Citation:

XIAO Dong-lai, ZHANG Di, LIN Yan-quan, YANG Jing. Evaluation on 20 Lentinula edodes Strains Using ISSR and F-MSAP Markers[J]. Fujian Journal of Agricultural Sciences, 2018, 33(8): 794-798. doi: 10.19303/j.issn.1008-0384.2018.08.005

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Evaluation on 20 Lentinula edodes Strains Using ISSR and F-MSAP Markers

doi: 10.19303/j.issn.1008-0384.2018.08.005

Institute of Edible & Medicinal Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China

Received Date: 2018-06-15
Rev Recd Date: 2018-07-18
Publish Date: 2018-08-01

Abstract

Abstract

The inter-simple sequence repeat (ISSR) and fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) markers were used to evaluate the genetic diversity of 20 Lentinula edodes strains. Ninety-eight bands were detected by 8 ISSR primers, of which 90.82% were polymorphic. The coefficient of pairwise genetic similarity ranged from 0.520 4 to 0.989 7.A total of 13 487 CCGG sites were detected using 15 selected primer pairs. Among the sites, 1 704 were of hemi-methylation with an average rate of 12.63%, and 1 865 of full-methylation with an average rate of 13.83%. The MSAP data were further divided into methylation sensitive polymorphisms (MSP) and methylation insensitive polymorphisms (MISP) groups. A significant correlation between ISSR and MISP was found by the Mantel test, while none between ISSR and MSP. It appeared that there was a significant methylation diversity among various L. edodes which could be of value for the breeding purpose.
- Lentinula edodes,
- ISSR,
- methylation,
- MSAP

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References(16)

References

[1]	ZIETKIEWICZ E, RAFSLSKI A, LABUDA D. Genome fingerprinting by simple sequence repeat(SSR)anchored polymerase chain re-action amplification[J]. Genomics, 1994, 20(2):176-183. doi: 10.1006/geno.1994.1151
[2]	陆娜, 周祖法, 王伟科, 等.利用ISSR分子标记鉴别杏鲍菇生产菌株的研究[J].北方园艺, 2015, 39(21):150-152. http://d.old.wanfangdata.com.cn/Periodical/bfyany201521038
[3]	LIU J, WANG Z R, LI C, et al. Evaluating genetic diversity and constructing core collections of Chinese Lentinula edodes cultivars using ISSR and SRAP markers[J]. Journal of Basic Microbiology, 2015, 55(6):749-760. doi: 10.1002/jobm.v55.6
[4]	刘晓红. ISSR辅助杏鲍菇常规杂交育种研究[D].合肥: 安徽农业大学, 2010. http://cdmd.cnki.com.cn/Article/CDMD-10364-1011024461.htm
[5]	LI W, WANG Y, ZHU J, et al. Differential DNA methylation may contribute to temporal and spatial regulation of gene expression and the development of mycelia and conidia in entomopathogenic fungus Metarhizium robertsii[J]. Fungal Biology, 2017, 121(3):293-303. doi: 10.1016/j.funbio.2017.01.002
[6]	肖冬来, 马璐, 张迪, 等.光照诱导广叶绣球菌基因组甲基化分析[J].食用菌学报, 2017, 24(4):6-11. http://d.old.wanfangdata.com.cn/Periodical/syjxb201704002
[7]	PENG H, JIANG G H, ZHANG J, et al. DNA methylation polymorphism and stability in Chinese indica hybrid rice[J]. Science China-Life Sciences, 2013, 56(12):1097-1106. doi: 10.1007/s11427-013-4576-z
[8]	ZHOU H, MA T Y, ZHANG R, et al. Analysis of different ploidy and parent-offspring genomic DNA methylation in the loach Misgurnus anguillicaudatus[J]. International Journal of Molecular Sciences, 2016, 17(8):1299. doi: 10.3390/ijms17081299
[9]	MÖLLER E M, BAHNWEG G, SANDERMANN H, et al. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues[J]. Nucleic Acids Research, 1992, 20(22):6115. doi: 10.1093/nar/20.22.6115
[10]	YANG T W, MA L H. Extracting DNA from edible fungus by combined method of CTAB and DNA gel purification Kit[J]. Biotechnology, 2009, 19(1):32-34. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=swjs200901012
[11]	ZHAO Y, CHEN M Y, STOREY K B, et al. DNA methylation levels analysis in four tissues of sea cucumber Apostichopus japonicus based on fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) during aestivation[J]. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2015, 181:26-32.
[12]	CERVERA M T, RUIZ-GARCÍA L, MARTÍNEZ-ZAPATER J. Analysis of DNA methylation in, Arabidopsis thaliana, based on methylation-sensitive AFLP markers[J]. Molecular Genetics and Genomics, 2002, 268(4):543-552. doi: 10.1007/s00438-002-0772-4
[13]	李毅丹.中国松嫩草原短芒野大麦[Hordeum brevisubulatum (Trin.) Link]人工种群的分子遗传与表观遗传多样性及其种群遗传结构的研究[D].长春: 东北师范大学, 2007.
[14]	ZHONG X F, WANG Y M, LIU X D, et al. DNA methylation polymorphism in annual wild soybean (Glycine soja Sieb. et Zucc.) and cultivated soybean (G. max L. Merr.)[J]. Canadian Journal of Plant Science, 2009, 89(5):851-863. doi: 10.4141/CJPS08215
[15]	FANG J G, SONG C N, QIAN J L, et al. Variation of cytosine methylation in 57 sweet orange cultivars[J]. Acta Physiology Plant, 2010, 32(6):1023-1030. doi: 10.1007/s11738-010-0491-0
[16]	刘靖宇, 宋秀高, 叶夏, 等.香菇菌株遗传多样性ISSR、RAPD和SRAP综合分析[J].食用菌学报, 2011, 18(3):1-8. doi: 10.3969/j.issn.1005-9873.2011.03.001