<i>Bacillus</i> Strains for Efficient Ammonium Nitrogen and Nitrite Removals on Frog Aquaculture Pond

TANG Jia-yi; WANG Xiao-jun; QIAN Xue-qiao; ZENG Jia-qi; XIAO Lan

doi:10.19303/j.issn.1008-0384.2018.07.002

Volume 33 Issue 7

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(7): 660-664

TANG Jia-yi, WANG Xiao-jun, QIAN Xue-qiao, ZENG Jia-qi, XIAO Lan. Bacillus Strains for Efficient Ammonium Nitrogen and Nitrite Removals on Frog Aquaculture Pond[J]. Fujian Journal of Agricultural Sciences, 2018, 33(7): 660-664. doi: 10.19303/j.issn.1008-0384.2018.07.002

Citation:

TANG Jia-yi, WANG Xiao-jun, QIAN Xue-qiao, ZENG Jia-qi, XIAO Lan. Bacillus Strains for Efficient Ammonium Nitrogen and Nitrite Removals on Frog Aquaculture Pond[J]. Fujian Journal of Agricultural Sciences, 2018, 33(7): 660-664. doi: 10.19303/j.issn.1008-0384.2018.07.002

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Bacillus Strains for Efficient Ammonium Nitrogen and Nitrite Removals on Frog Aquaculture Pond

doi: 10.19303/j.issn.1008-0384.2018.07.002

1.
Animal Husbandry and Fisheries Research Center of Guangdong Haida Group Co., Ltd. /Key Laboratory of Microecological Resources and Utilization in Breeding Industry, Ministry of Agriculture, Guangzhou, Guangdong 511400, China
2.
College of Environment and Energy, South China University of Technology, Guangzhou, Guangdong 510006, China

Received Date: 2018-04-25
Rev Recd Date: 2018-06-12
Publish Date: 2018-07-01

Abstract

Abstract

To effectively detox the water in a frog aquaculture pond, Bacillus bacteria capable of degrading NH₄⁺-N and NO₂^--N were collected from the ponds for screening and evaluation. Strain B2 was selected from 15 isolated candidates for this study based upon its superior nitrogen removal and enzyme producing capabilities. It showed 64.56% reduction on NH₄⁺-N and 36.39% on NO₂^--N in 5 days and adequately produced functional enzymes under either aerobic or anaerobic conditions that might present in a frog pond. The physiological and biochemical characteristics and the 16S rDNA sequence of B2 confirmed it to be a strain of Bacillus subtilis. The optimum conditions for NH₄⁺-N and NO₂^--N degradation by B2 ranged from 25℃ to 35℃ at pH 6 to 8. Further study to identify and finetune the application is in order.
- frog aquaculture,
- organic matters,
- Bacillus,
- isolation and screening

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References(17)

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