Cloning and Expression of Phytoene Desaturase Gene, <i>PpPDS</i>, from Peaches

LIANG Min-hua; YANG Zhen-feng; SU Xin-guo; SONG Chun-bo

doi:10.19303/j.issn.1008-0384.2018.06.012

Volume 33 Issue 6

Mar. 2019

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LIANG Min-hua, YANG Zhen-feng, SU Xin-guo, SONG Chun-bo. Cloning and Expression of Phytoene Desaturase Gene, PpPDS, from Peaches[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 608-613. doi: 10.19303/j.issn.1008-0384.2018.06.012

Citation:

LIANG Min-hua, YANG Zhen-feng, SU Xin-guo, SONG Chun-bo. Cloning and Expression of Phytoene Desaturase Gene, PpPDS, from Peaches[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 608-613. doi: 10.19303/j.issn.1008-0384.2018.06.012

Citation:

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Cloning and Expression of Phytoene Desaturase Gene, PpPDS, from Peaches

doi: 10.19303/j.issn.1008-0384.2018.06.012

1.
School of Food Science, Guangdong Food and Drug Vocational College, Guangzhou, Guangdong 510520, China
2.
College of Biological and Environmental Sciences, Zhejiang Wanli university, Ningbo, Zhejiang 315100, China
3.
College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510640, China

Received Date: 2018-01-12
Rev Recd Date: 2018-04-12
Publish Date: 2018-06-01

Abstract

Abstract

The full-length cDNAs of phytoene desaturase (PDS) from peaches was isolatedby gene cloning, and named as PpPDS. It was 2 056 bp in length and encoded a predicted protein of 573 amino acids (ORF length at 1 722 bp). A phylogenetic analysis indicated that PpPDS closely resembled the PDSs from Prunus armeniaca and Fragaria×ananassa. The deduced amino acid sequence suggested that PpPDS protein contained dinucleotide binding motifs, NAD (H), NADP (H) or FAD (H), which belonged to the NAD(P)-binding rossmann-like conserved domain. Quantitative real-time PCR showed that PpPDS gene was generally expressed higher in mesocarp than exocarp in fruits of Jinli yellow peach at different maturity stages. After the peach stone hardened, the gene expression increased significantly, then leveled off at the peak. The information would be useful for further studies on the carotenoid biosynthesis pathway in peaches.
- peach,
- PDS,
- gene cloning,
- expression analysis

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References(20)

References

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