Preparation and Characterization Analysis of Monoclonal Antibody Against the Avian Tembusu Virus

SHI Shao-hua; CHENG Long-fei; FU Guang-hua; CHEN Zhen; FU Qiu-ling; LIU Rong-chang; WAN Chun-he; CHEN Hong-mei; HUANG Yu

doi:10.19303/j.issn.1008-0384.2018.06.001

Volume 33 Issue 6

Mar. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(6): 551-555

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Zhen, FU Qiu-ling, LIU Rong-chang, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Preparation and Characterization Analysis of Monoclonal Antibody Against the Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 551-555. doi: 10.19303/j.issn.1008-0384.2018.06.001

Citation:

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Zhen, FU Qiu-ling, LIU Rong-chang, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Preparation and Characterization Analysis of Monoclonal Antibody Against the Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 551-555. doi: 10.19303/j.issn.1008-0384.2018.06.001

Citation:

SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Zhen, FU Qiu-ling, LIU Rong-chang, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Preparation and Characterization Analysis of Monoclonal Antibody Against the Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 551-555. doi: 10.19303/j.issn.1008-0384.2018.06.001

PDF( 1373 KB)

Preparation and Characterization Analysis of Monoclonal Antibody Against the Avian Tembusu Virus

doi: 10.19303/j.issn.1008-0384.2018.06.001

Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian 350013, China

Received Date: 2018-01-06
Rev Recd Date: 2018-04-16
Publish Date: 2018-06-01

Abstract

Abstract

This study aimed to prepare and characterize monoclonal antibody (MAb) against avian Tembusu virus. BALB/c mice were immunized with the antigen of purified avian Tembusu virus, and then two MAbs named as 2F6 and 4E11 were selected. The two MAbs, with the titers of up to 1:64, 000, were reacted with Vero cells infected with avian Tembusu virus. The Western blot analysis showed that both MAbs 2F6 and 4E11 were able to react with fusion envolope protein by prokaryotic expression, indicating that the two MAbs recognized the envelope protein. The prepared MAbs could be used to further explore the molecular epidemiology and development of new diagnostic methods for avian Tembusu virus infection.
- Avian Tembusu virus,
- monoclonal antibody,
- envelope protein

FullText(HTML)

References(20)

References

[1]	万春和, 施少华, 程龙飞, 等.一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定[J].福建农业学报, 2010, 25(6):663-666. http://cpfd.cnki.com.cn/Article/CPFDTOTAL-ZXMY201509002041.htm
[2]	SU J, LI S, HU X, et al.Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related Flavivirus[J].PLoS One, 2011, 6(3):e18106. doi: 10.1371/journal.pone.0018106
[3]	CAO Z, ZHANG C, LIU Y, et al. Tembusu virus in ducks, China[J]. Emerg Infect Dis, 2011, 17(10):1873-1875. doi: 10.3201/eid1710.101890
[4]	YAN P, ZHAO Y, ZHANG X, et al. An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China[J].Virology, 2011, 417(1):1-8. doi: 10.1016/j.virol.2011.06.003
[5]	傅光华, 黄瑜, 施少华, 等.鸡黄病毒的分离与初步鉴定[J].福建畜牧兽医, 2011, 33(3):1-2. https://www.cnki.com.cn/qikan-ANHE200911148.html
[6]	陈仕龙, 陈少莺, 王劭, 等.一种引起蛋鸡产蛋下降的新型黄病毒的分离与初步鉴定[J].福建农业学报, 2011, 26(2):170-174. https://www.wenkuxiazai.com/doc/794261e4aeaad1f346933f9e.html
[7]	黄欣梅, 李银, 赵冬敏, 等.新型鹅黄病毒JS804毒株的分离与鉴定[J].江苏农业学报, 2011, 27(2):354-360. https://www.doc88.com/p-4827428300908.html
[8]	施少华, 傅光华, 万春和, 等.鹅源坦布苏病毒的分离及初步鉴定[J].中国兽医杂志, 2012, 48(12):10-11. http://www.cqvip.com/QK/92143X/201212/44477664.html
[9]	黄瑜, 苏荣茂, 傅光华, 等.禽坦布苏病毒感染的宿主及临床表现[J].中国兽医杂志, 2014, 50(11):50-52. doi: 10.3969/j.issn.0529-6005.2014.11.016
[10]	MUKHOPADHYAY S, KUHN R J, ROSSMANN M G.A structural perspective of the flavivirus life cycle[J]. Nat Rev Microbiol, 2005, 3(1):13-22. doi: 10.1038/nrmicro1067
[11]	邓永强, 秦鄂德.虫媒黄病毒包膜E蛋白的研究进展[J].军事医学科学院院刊, 2006, 30(6):575-579. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_jsyxkxyyk200606021
[12]	王丹丹, 张曦, 郎咸勇, 等.禽坦布苏病毒截短E蛋白单克隆抗体的制备及其抗原性分析[J].中国兽医科学, 2016, 46(7):805-813. http://www.cqvip.com/QK/93994X/201607/669535015.html
[13]	孙涛, 王超, 徐彪, 等.鸭坦布苏病毒E基因主要抗原域的原核表达及单克隆抗体的制备[J].中国预防兽医学报, 2016, 38(6):496-499. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgyfsyxb201606017
[14]	白小飞, 刘红玉, 陈玉环, 等.抗鸭坦布苏病毒E蛋白单克隆抗体的制备及鉴定[J].中国兽医科学, 2014, 44(8):833-837. http://wuxizazhi.cnki.net/Sub/YZJS/a/XMYS201310003.html
[15]	林源, 柳金雄, 陈普成, 等.鸭坦布苏病毒E蛋白单克隆抗体的制备及鉴定[J].畜牧与兽医, 2013, 43(10):6-9. http://wuxizazhi.cnki.net/Sub/YZJS/a/XMYS201310003.html
[16]	孙东波, 冯力, 时洪艳, 等.猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定[J].中国预防兽医学报, 2007, 29(11):887-890. doi: 10.3969/j.issn.1008-0589.2007.11.014
[17]	巴德山.现代免疫学实验技术[M].北京:北京医科大学中国协和医科大学联合出版社, 1988:158-162.
[18]	曹雪涛.精编免疫学实验指南[M].北京:科学出版社, 2009:120-123.
[19]	施少华, 程龙飞, 傅光华, 等.鸭坦布苏病毒囊膜E蛋白的原核表达[J].福建农业学报, 2014, 29(10):935-938. doi: 10.3969/j.issn.1008-0384.2014.10.001
[20]	KONO Y, TSUKAMOTO K, ABD HAMID M, et al.Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus[J]. Trop Med Hyg, 2000, 63(1):94-101. doi: 10.4269/ajtmh.2000.63.94