Artificially Synthesized Salidroside Antigen

WANG Wei; HU Li-xin; FU Guang-hua

doi:10.19303/j.issn.1008-0384.2018.05.016

Volume 33 Issue 5

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(5): 530-533

WANG Wei, HU Li-xin, FU Guang-hua. Artificially Synthesized Salidroside Antigen[J]. Fujian Journal of Agricultural Sciences, 2018, 33(5): 530-533. doi: 10.19303/j.issn.1008-0384.2018.05.016

Citation:

WANG Wei, HU Li-xin, FU Guang-hua. Artificially Synthesized Salidroside Antigen[J]. Fujian Journal of Agricultural Sciences, 2018, 33(5): 530-533. doi: 10.19303/j.issn.1008-0384.2018.05.016

WANG Wei, HU Li-xin, FU Guang-hua. Artificially Synthesized Salidroside Antigen[J]. Fujian Journal of Agricultural Sciences, 2018, 33(5): 530-533. doi: 10.19303/j.issn.1008-0384.2018.05.016

Citation:

WANG Wei, HU Li-xin, FU Guang-hua. Artificially Synthesized Salidroside Antigen[J]. Fujian Journal of Agricultural Sciences, 2018, 33(5): 530-533. doi: 10.19303/j.issn.1008-0384.2018.05.016

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Artificially Synthesized Salidroside Antigen

doi: 10.19303/j.issn.1008-0384.2018.05.016

Linyi Institute for Food and Drug Control, Linyi, Shandong 276000, China

Received Date: 2018-01-22
Rev Recd Date: 2018-04-17
Publish Date: 2018-05-01

Abstract

Abstract

To establish an immunoassay method on salidroside (SAL), it is necessary to firstly create a complete SAL antigen. This study applied the sodium periodate oxidation method to synthesize the immunizing antigen, SAL-BSA, and the coating antigen, SAL-OVA, which were positively identified by means of ultraviolet spectrum and SDS-PAGE. Subsequently, lab mice were immunized with the SAL-BSA to produce antiserum against SAL. The antibody titer was, then, determined by indirect ELISA. The results showed that SAL was conjugated with BSA/OVA in a ratio of 7:1, and the serum antigen titer reached 1:4 000. With the complete SAL antigen successfully synthesized, the preparation of monoclonal antibodies for the establishment of an applicable immunoassay methodology became possible.
- salidroside,
- artificial antigen,
- antibody

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References(20)

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