Isolation, Identification and Antibacterial Tests on <i>Penicillium citrinum</i> for Postharvest Shengzhou Plums

ZHAO Ning; SONG Yu-hui; CHEN Li; MO Yi-wei

doi:10.19303/j.issn.1008-0384.2018.04.014

Volume 33 Issue 4

Mar. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(4): 407-412

ZHAO Ning, SONG Yu-hui, CHEN Li, MO Yi-wei. Isolation, Identification and Antibacterial Tests on Penicillium citrinum for Postharvest Shengzhou Plums[J]. Fujian Journal of Agricultural Sciences, 2018, 33(4): 407-412. doi: 10.19303/j.issn.1008-0384.2018.04.014

Citation:

ZHAO Ning, SONG Yu-hui, CHEN Li, MO Yi-wei. Isolation, Identification and Antibacterial Tests on Penicillium citrinum for Postharvest Shengzhou Plums[J]. Fujian Journal of Agricultural Sciences, 2018, 33(4): 407-412. doi: 10.19303/j.issn.1008-0384.2018.04.014

Citation:

PDF( 2955 KB)

Isolation, Identification and Antibacterial Tests on Penicillium citrinum for Postharvest Shengzhou Plums

doi: 10.19303/j.issn.1008-0384.2018.04.014

ZHAO Ning^1
,,
SONG Yu-hui²,
CHEN Li¹,
MO Yi-wei^{1
,
,}

1.
College of Life Science, Shaoxing University, Shaoxing, Zhejiang 312000, China
2.
Shaoxing No.1 High School, Shaoxing, Zhejiang 312000, China

Received Date: 2018-02-01
Rev Recd Date: 2018-03-13
Publish Date: 2018-04-01

Abstract

Abstract

The renowned Shengzhou plums (Prunus salicina Lindl. var. taoxingli) ripen in the summer, and are high on nutritional value and appealing on eating quality. However, they turn soft rapidly and are prone to deterioration after harvesting in the summer heat. This study attempted to identify the source of microbial infection and determine an effective antibacterial measure to extend the shelf life for the prized fruits. Using the rDNA-ITS molecular identification and morphology methods, a pathogen was isolated from the samples of diseased fruits. In the lab, the isolate was treated with Sporgon and difenoconazole+azoxystrobin to confirm it to be Penicillium citrinum (TXL-03). The fungus had numerous branches and conidiophores. It could infect a wide variety of fruits, such as pear, apple, nectarine, cherry, tomato, grape, mango, plum, and banana. Either Sporgon or difenoconazole+azoxystrobin could retard the fungal growth. However, Sporgon appeared more effective, and thus, was recommended for treatment of the disease.
- plum,
- Penicillium citrinum,
- isolation and identification,
- antibacterial test

FullText(HTML)

References(23)

References

[1]	孟祥春, 毕方铖, 丁心, 等.茶树油粕粗提物对柑橘青绿霉病发生的抑制作用[J].中国生物防治学报, 2015, 31(2): 236-241. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgswfz201502012
[2]	郜海燕, 肖尚月, 陈杭君, 等.蓝莓采后主要病原真菌的分离鉴定及生物学特性研究[J].农业机械学报, 2017, 48(5):327-334. doi: 10.6041/j.issn.1000-1298.2017.05.041
[3]	周春元, 李玉.4株有害疣孢霉菌的生物学特性研究[J].西北农林科技大学学报(自然科学版), 2015, 43(6):199-204. http://www.cnki.com.cn/Article/CJFDTotal-XBNY201506030.htm
[4]	何莹蓉, 林海雁, 曹宗威, 等.不同处理对桃形李采后病原菌的防治效果[J].热带作物学报, 2017, 38(4): 734-739. http://www.cnki.com.cn/Article/CJFDTOTAL-SPFX201703043.htm
[5]	王渊, 张芳, 韩丽丽, 等.6种杀菌剂对4种常见植物病原菌的抑制作用[J].干旱区资源与环境, 2013, 21(11):92-97. doi: 10.3969/j.issn.1003-7578.2013.11.016
[6]	李平东, 陈雪君. 6种杀菌剂对香蕉采后炭疽病防治效果研究[J].安徽农业科学, 2009, 37(18):8567-8568. doi: 10.3969/j.issn.0517-6611.2009.18.108
[7]	农秀美, 黄红, 蔡健和, 等.施保功和施保克对芒果贮藏期炭疽病的控制试验简报[J].广西农业科学, 1994(4):173-174. http://www.cqvip.com/QK/90177X/199404/1345303.html
[8]	高苏娟, 谭文兴, 陈俊卫, 等.施保功、扑海因和热处理对紫金春甜桔采后贮藏效果的影响[J].广东农业科学, 2012(19):87-89. doi: 10.3969/j.issn.1004-874X.2012.19.029
[9]	朱迎迎, 高兆银, 李敏, 等.火龙果镰刀菌果腐病病原菌鉴定及生物学特性研究[J].热带作物学报, 2016, 37(1):164-171. http://www.cnki.com.cn/Article/CJFDTotal-RDZX201601028.htm
[10]	臧威, 孙翔, 孙剑秋, 等.南方红豆杉内生真菌的多样性与群落结构[J].应用生态学报, 2014, 25(7):2071-2078. http://www.cjae.net/CN/abstract/abstract19645.shtml
[11]	姚昕, 涂勇. 8种杀菌剂对雪莲果褐斑病的室内毒力测定[J].西昌学院学报(自然科学版), 2014, 28(4):22-24. http://www.cnki.com.cn/Article/CJFDTotal-XCNY201404008.htm
[12]	范青生, 马振亚.防腐抑菌中药的微量快速筛选法研究及其应用[J].微生物学通报, 1991, 18(2):114-118. http://www.cqvip.com/QK/94824X/199102/624749.html
[13]	De CLERCQ N, VLAEMYNCK G, Van PAMEL E.Isoepoxydon dehydrogenase(idh) gene expression in relation to patulin production by Penicillium expansum under different temperature and atmosphere[J]. International Journal of Food Microbiology, 2016, 220(4): 50-57. https://biblio.ugent.be/publication/7153507
[14]	REDDY K R N, SPADARO D, LORE A, et al. Potential of patulin production by Penicillium expansum strains on various fruits[J]. Mycotoxin Research, 2010, 26(4):257-265. doi: 10.1007/s12550-010-0064-5
[15]	姜辣, 王丽婷, 陈海燕, 等.壳寡糖对扩展青霉菌体生长和毒素分泌量的影响[J].食品科学技术学报, 2015, 33(5):23-30. doi: 10.3969/j.issn.2095-6002.2015.05.005
[16]	郝晓娟, 高莹, 李新凤, 等.欧李褐腐病病原菌生物学特性及其寄主范围[J].果树学报, 2014, 31(1):101-104. http://www.cnki.com.cn/Article/CJFDTOTAL-ZJNY198504011.htm
[17]	SMILANICK J L, MACKEY B E, REESE R, et al. Influence of concentration of soda ash, temperature, and immersion period on the control of postharvest green mold of oranges[J]. Plant Disease, 1997, 81(4):379-382. doi: 10.1094/PDIS.1997.81.4.379
[18]	LAI T, BAI X, WANG Y, et al. Inhibitory effect of exogenous sodium bicarbonate on developmentand pathogenicity of postharvest disease Penicillium expansum[J]. Scientia Horticulturae, 2015, 187:108-114. doi: 10.1016/j.scienta.2015.03.010
[19]	ZHONG Y Y, LIi B Q, TIAMian S P. Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum[J]. International Journal of Food Microbiology, 2015, 206(2):102-108. http://cn.bing.com/academic/profile?id=3fce0933b44d2cac0e4572950efce5d2&encoded=0&v=paper_preview&mkt=zh-cn
[20]	黄涛江, 曾安中, 夏凯峰, 等.几种保鲜剂对'鲍威尔'脐橙冷藏效果的影响[J].中国园艺文摘, 2014, 30(12):3-5. doi: 10.3969/j.issn.1672-0873.2014.12.002
[21]	钟祯凯, 康浩, 吴诚, 等.咪鲜胺和吡唑醚菌酯对杧果炭疽病菌的复配作用研究[J].中国南方果树, 2016, 46(1):59-61. https://www.cnki.com.cn/lunwen-1013327216.html
[22]	唐靖文, 陈春旭, 龙友华, 等.6种杀菌剂对批把灰斑病的室内毒力测定[J].中国园艺文摘, 2016, 32(4):51-53. http://www.cqvip.com/QK/60020X/201604/668548768.html
[23]	卢植新, 林明珍, 黄辉晔, 等.施保功50%WP浸芒果防腐保鲜后的残留消解动态及安全性评价[J].中国生态农业学报, 2007, 2(15):96-98. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=stnyyj200702026