Research Advances on Ecological Risk of Transgenic Bt Rice on Non-Target Insects

QIU Liang-miao; LIU Qi-quan; LIN Ren-kui; SHI Long-qing; ZHAN Zhi-xiong

doi:10.19303/j.issn.1008-0384.2018.03.019

Volume 33 Issue 3

Mar. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(3): 326-333

QIU Liang-miao, LIU Qi-quan, LIN Ren-kui, SHI Long-qing, ZHAN Zhi-xiong. Research Advances on Ecological Risk of Transgenic Bt Rice on Non-Target Insects[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 326-333. doi: 10.19303/j.issn.1008-0384.2018.03.019

Citation:

QIU Liang-miao, LIU Qi-quan, LIN Ren-kui, SHI Long-qing, ZHAN Zhi-xiong. Research Advances on Ecological Risk of Transgenic Bt Rice on Non-Target Insects[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 326-333. doi: 10.19303/j.issn.1008-0384.2018.03.019

Citation:

PDF( 961 KB)

Research Advances on Ecological Risk of Transgenic Bt Rice on Non-Target Insects

doi: 10.19303/j.issn.1008-0384.2018.03.019

QIU Liang-miao^{1, 2, 3
,},
LIU Qi-quan^{1, 2, 3},
LIN Ren-kui⁴,
SHI Long-qing^{2, 5},
ZHAN Zhi-xiong^{2, 3, 5
,
,}

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3.
Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou, Fujian 350013, China
4.
Xianyou Plant Protection and Plant Quarantine Station, Xianyou Fujian 351200, China
5.
Rice Research Institute of Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China

Received Date: 2017-12-12
Rev Recd Date: 2018-02-14
Publish Date: 2018-03-01

Abstract

Abstract

Genes of Bacillus thuringiensis (Bt) are the most studied and applied genetic material for transgenic plant bioengineering research. For instance, the transgenic Bt rice is highly effective in controlling lepidopterous pests, such as the striped stem borer, yellow stem borer and rice leaf roller. Consequently, commercial cultivation of Bt rice has become a focal point for the scientists in China and abroad. This article reviews current progress on the related studies with an emphasize on the ecological effect of Bt rice on non-target insects in the field. Future development and research concerning the issues are discussed.
- insect-resistant transgenic rice,
- Bt gene,
- non-target insects,
- ecological risk,
- progress

FullText(HTML)

References(104)

References

[1]	朱祯.高效抗虫转基因水稻的研究与开发[J].中国科学院院刊, 2001, 16(5): 353-357. https://www.wenkuxiazai.com/doc/7888310d680203d8ce2f24f6.html
[2]	唐丽, 谭炎宁, 韩小霞, 等.抗虫转基因水稻研究进展及发展趋势[J].杂交水稻, 2011, 26(1): 1-6. http://www.cqvip.com/QK/94823X/1999009/3704615.html
[3]	盛承发, 王红托, 高留德, 等.我国水稻螟虫大发生现状、损失估计及防治对策[J].植物保护, 2003, 29(1): 37-39. http://www.cqvip.com/QK/90218X/200301/7420851.html
[4]	王忠华, 舒庆尧, 夏英武.基因工程在水稻改良方面的研究进展[J].生物技术通报, 1999, 2(2): 5-8. http://www.cnki.com.cn/Article/CJFDTOTAL-SWJT902.001.htm
[5]	王继磊, 刘迪秋, 丁元明, 等. Bt转基因抗虫植物研究进展[J].生物学杂志, 2010, 27(4): 75-78. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=swxzz201004022
[6]	王丽冰, 刘立军, 颜亨梅.转Bt抗虫基因水稻的研究进展和生物安全性及其对策[J].生命科学研究, 2009, 13(2):182-188. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=smkxyj200902018
[7]	傅强, 赖凤香, 陈洋, 等.抗虫转基因水稻对非靶标生物的生态安全性研究进展[J].植物生理学报, 2013, 49(7): 655-663. http://www.cnki.com.cn/Article/CJFDTOTAL-YCZZ201105002.htm
[8]	杨虹, 李家新, 郭三维, 等.苏云金芽孢杆菌δ-内毒素基因导入水稻原生质体后获得转基因植株[J].中国农业科学, 1989, 22(6): 1-5. http://www.cqvip.com/QK/90161X/198906/189173.html
[9]	RAO K V, RATHORE K S, HODGES T K, et al. Expression of snowdrop lectin(GNA) in transgenic rice plants confers resistance to rice brown plant hopper[J]. Plant J, 1998(14): 469-477.
[10]	何锶洁, 徐琼芳, 唐祚舜, 等.天花粉蛋白基因在水稻中转化及抗虫研究[J].高技术通讯, 2000, 10(5): 8-10. http://mall.cnki.net/magazine/Article/GJSX200005001.htm
[11]	SHU Q Y, YE G Y, CUI H R, et al. Transgenic rice plants with a synthetic Cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species[J]. Mol Breeding, 2000, 6(4): 433-439. doi: 10.1023/A:1009658024114
[12]	TANG K, ZHAO E, SUN X, et al. Production of transgenic rice homozygous lines with enhanced resistance to the rice brown plant hopper[J]. Acta Biotechnol, 2001, 21(2): 117-128. doi: 10.1002/(ISSN)1521-3846
[13]	李桂英, 许新萍, 夏嫱, 等.转GNA+SBTi双价基因抗虫水稻的遗传分析及抗虫性研究[J].中山大学学报, 2003, 42(1): 125-126. http://cdmd.cnki.com.cn/Article/CDMD-10736-2002122379.htm
[14]	JING G H, XU C G, TU M, et al. Pyramiding of insect and disease-resistance genes into an elite indica, cytoplasm male sterile restorer line of rice, 'Minghui 63'[J]. Plant Breed, 2004, 123: 112-116. doi: 10.1046/j.1439-0523.2003.00917.x
[15]	RAO M V R, BEHERA K S, BAISAKH N, et al. Transgenic indica rice cultivar 'Swarna'expressing a potato chymotrypsin inhibitor pin2 gene show enhanced levels of resistance to yellow stem borer[J]. Plant Cell, Tissue and Organ Cult, 2009, 99(3): 277-285. doi: 10.1007/s11240-009-9602-2
[16]	BRAVO A, GILL S S, SOBERON M. Mode of action of Bacillus Thuringiensis Cry and Cyt toxin and their potential for insect control[J]. Toxicon, 2007, 49(4): 423-435.
[17]	梁革梅, 王桂荣, 徐广, 等.昆虫Bt毒素受体蛋白的研究进展[J].昆虫学报, 2003, 46(3): 390-396. http://www.cnki.com.cn/Article/CJFDTOTAL-KCXB200303019.htm
[18]	LEE M K, WALTERS F S, HART H, et al. The mode of action of the Bacillus thuringiensis vegetative insecticidal protein Vip3A differs from that of Cry1Ab δ-endotoxin[J]. Applied and Environmental Microbiology, 2003, 69(8): 4648-4657. doi: 10.1128/AEM.69.8.4648-4657.2003
[19]	SCHNEPF H E, CRICKMORE N, VAN RIE J, et al. Bacillus thuringiensis and its pesticidal crystal proteins[J]. Microbiol Mol Biol Rev, 1998, 62(3): 775-806. http://cn.bing.com/academic/profile?id=28584276131c75888fcb50455ebf5dc1&encoded=0&v=paper_preview&mkt=zh-cn
[20]	FUJINOTO H, ITOH K, YAMNMOTO M, KYOZUKA J, et al. Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus Thuringiensis[J]. Biotechnology, 1993(11): 1151-1155. http://cn.bing.com/academic/profile?id=e1d157cb2d369a08fb03d4fc16037c8d&encoded=0&v=paper_preview&mkt=zh-cn
[21]	NAYAK P, BASU D, DAS S, et al. Transgenic elite indica rice plants expressing Cry1Ac δ-endotoxin of Bacillus thuringiensis are resistant against yellow stem borer(Scirpophagq incertulas)[J]. Proc Natl Acad Sci USA, 1997, 94: 2111-2116. doi: 10.1073/pnas.94.6.2111
[22]	DATTA K, VASQUEZ A, TU J, et al. constitutive and tissue specific differential expression of the Cry1Ab in transgenic rice plants conferring resistance to rice insect pests[J]. Theor Appl Genet, 1998, 97: 31-36. doi: 10.1007/s001220050863
[23]	崔彦芹, 李尚伟, 张丽萍, 等.双价抗虫转基因水稻的育成及初步鉴定[J].南方农业学报, 2016, 47(2): 169-173. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gxnykx201602002
[24]	韩兰芝, 吴孔明, 彭于发, 等.转基因抗虫水稻生态安全性研究进展[J].应用与环境生物学报, 2006, 12(3): 431-436. http://www.cqvip.com/qk/98345X/200603/22205131.html
[25]	陈浩, 林拥军, 张启发.转基因水稻研究的回顾与展望[J].科学通报, 2009, 54(18): 2699-2717. http://mall.cnki.net/magazine/Article/KXTB200918011.htm
[26]	TU J, ZHANG G, DATTA K, et al. Field performance of transgenic elite commercial hybrid rice expressing Bacillus thuringiensis δ-endotoxin[J]. Nat Biotechnol, 2000, 18(10): 1101-1104. doi: 10.1038/80310
[27]	舒庆尧, 叶恭银, 崔海瑞, 等.转基因水稻"克螟稻"选育[J].浙江农业大学学报, 1998, 24(6). 579-580. http://www.cnki.com.cn/Article/CJFDTOTAL-ZJNY806.002.htm
[28]	朱祯.转基因水稻研究进展[J].中国农业科技导报, 2010, 12(2): 9-16. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ahnykx201109029
[29]	CHEN M, SHELTON A, YE G Y. Insect-resistant genetically modified rice in China: from research to commercialization[J]. Annual Review of Entomology, 2011, 56: 81-101. doi: 10.1146/annurev-ento-120709-144810
[30]	张富丽, 刘勇, 佟洪金, 等.不同虫压下转Bt基因水稻与非转基因水稻生态适合度差异[J].应用与环境生物江学报, 2012, 18(1): 35-41. http://www.cibj.com/oa/DArticle.aspx?type=view&id=201107028
[31]	徐雪亮, 肖叶青, 季香云, 等.转Bt基因水稻"赣绿1号"对非靶标害虫褐飞虱取食、产卵行为及生长发育的影响[J].应用昆虫学报, 2015, 52(4): 977-982. doi: 10.7679/j.issn.2095-1353.2015.117
[32]	BERNAL C C, AGUDA R M, COHEN M B. Effect of rice lines transformed with Bacillus thuringiensis toxin genes on the brown planthopper and its predator Cyrtorhinus lividipennis[J]. Entomol Exp Appl, 2002, 102: 21-28. doi: 10.1046/j.1570-7458.2002.00921.x
[33]	傅强, 王锋, 李东虎, 等.转基因抗虫水稻MSA和MSB对非靶标害虫褐飞虱和白背飞虱的影响[J].昆虫学报, 2003, 46(6): 697-704. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_kcxb200306006
[34]	高明清, 侯守鹏, 蒲德强, 等.田间转Bt基因水稻上稻飞虱卵量、孵化率及天敌作用[J].昆虫学报, 2011, 54(4):467-476. http://mall.cnki.net/magazine/Article/KCXB201104013.htm
[35]	董亚强, 李强, 尤民生, 等.转Cry1Ab抗虫水稻对褐飞虱取食及产卵选择行为的影响[J].福建农林大学学报(自然科学版), 2016, 45(3): 252-256. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fjnydxxb201603003
[36]	陈茂, 叶恭银, 姚洪渭, 等.抗虫转基因水稻对非靶标害虫褐飞虱取食与产卵行为影响的评价[J].中国农业科学, 2004, 37(2): 222-226. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgnykx200402010
[37]	CHEN Y, TIAN J C, WANG W, et al. Bt rice expressing Cry1Ab does not stimulate an outbreak of its non-target herbivore, Nilaparvata lugens[J]. Transgenic Res, 2012, 21: 279-291. doi: 10.1007/s11248-011-9530-x
[38]	刘丰静, 陈文滨, 林胜, 等.转基因抗虫杂交稻对非靶标害虫稻飞虱田间种群动态的影响[J].华东昆虫学报2007, 16(1): 8-12. http://cdmd.cnki.com.cn/Article/CDMD-10389-2007135676.htm
[39]	谭红, 叶恭银, 沈君辉, 等.转Cry1Ab基因抗虫籼稻对非靶标害虫白背飞虱发育与繁殖的影响[J].植物保护学报, 2006, 33(3): 251-256. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwbhxb200603006
[40]	CHEN M, ZHAO J Z, YE G Y, et al. Impact of insect-resistant transgenic rice on target insect pests and non-target arthropods in China[J]. Insect Sci, 2006, 13: 409-420. doi: 10.1111/ins.2006.13.issue-6
[41]	CHEN M, LIU Z C, YE G Y, et al. Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis(Hemiptera: Miridae)-a case study of the compatibility of Bt rice with biological control[J]. Biol Control, 2007, 42: 242-250. doi: 10.1016/j.biocontrol.2007.05.005
[42]	刘雨芳, 贺玲, 汪琼, 等.转Cry1Ab/sck基因抗虫水稻对稻田主要非靶标害虫的田间影响评价[J].中国农业科学, 2007, 40(6): 1181-1189. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgnykx200706013
[43]	陈洋, 田俊策, 彭于发, 等.转Cry1Ab/Vip3H基因水稻对非靶标害虫褐飞虱连续多代生长发育与繁殖的影响[J].中国生物防治学报, 2011, 27(4): 490-497. http://www.ag.udel.edu/delpha/8718.pdf
[44]	隋贺, 李志毅, 徐艳博, 等.广西兴安转Bt水稻大田两迁害虫发生动态[J].应用生态学报, 2011, 22(11): 3021-3025. http://www.cjae.net/CN/abstract/abstract18329.shtml
[45]	梁玉勇, 程正新, 程森弟, 等.水稻对褐飞虱及其二种天敌种群动态的影响[J].应用昆虫学报, 2014, 51(5): 1163-1172. doi: 10.7679/j.issn.2095-1353.2014.138
[46]	刘志诚, 叶恭银, 胡萃, 等.转Cry1 Ab/Cry1 Ac基因籼稻对稻田节肢动物群落影响[J].昆虫学报, 2003, 46(4):454-465. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH199906019.htm
[47]	刘雨芳.抗虫水稻与研究进展与生态安全评价[J].生命科学研究, 2004, 8(4): 294-299. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_smkxyj200404002
[48]	WANG Y M, ZHANG G A, DU J P, et al. Influence of transgenic hybrid rice expressing a fused gene derived from Cry1Ab and Cry1Ac on primary insect pests and rice yield[J]. Crop Prot, 2010, 29: 128-133. doi: 10.1016/j.cropro.2009.10.004
[49]	刘志诚, 叶恭银, 胡萃, 等. Bt水稻对主要非靶标害虫和蜘蛛优势种田间种群动态的影响[J].植物保护学报, 2002, 29(2): 8-14. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zwbhxb200202010
[50]	周霞, 程家安, 胡阳, 等.转Bt基因水稻克螟稻对黑尾叶蝉种群增长的影响[J].中国水稻科学, 2005, 19(1): 74-78. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGSK20050100D.htm
[51]	CHEN M, YE G Y, LIU Z C, et al. Field assessment of the effects of transgenic rice expressing a fused gene of Cry1Ab and Cry1Ac from Bacillus thuringiensis Berliner on non-target planthoppers and leafhopper populations[J]. Environ Entomol, 2006, 35: 127-134. doi: 10.1603/0046-225X-35.1.127
[52]	唐健, 杨保军, 蒋跃南, 等.稻蓟马危害转Bt基因水稻克螟稻2号研究初报[J].中国水稻科学, 2000, 14(4): 241-242. http://www.cqvip.com/qk/97017X/200004/4592261.html
[53]	陈茂, 叶恭银, 胡萃, 等. Bt水稻对飞虱和叶蝉及其卵寄生蜂扩散规律的影响[J].浙江大学学报(农业与生命科学版), 2003, 29(1):29-33. http://www.cqvip.com/QK/94288A/2003001/7354215.html
[54]	刘雨芳, 苏军, 尤民生, 等.转基因抗虫水稻对水稻害虫群落的影响[J].昆虫学报, 2005, 48(4): 544-553. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kcxb200504012
[55]	姜永厚, 傅强, 程家安, 等.转Bt基因水稻表达的毒蛋白Cry1Ab在害虫及其捕食者体内的积累动态[J].昆虫学报, 2004, 47(4): 454-460. http://www.cqvip.com/QK/94065X/200404/10113866.html
[56]	CHEN H, LIN Y J, ZhHANG Q F. Review and prospect of transgenic rice research[J]. Chin Sci Bull, 2009, 54:4049-4068. doi: 10.1007/s11434-009-0645-x
[57]	刘立军. 转基因水稻表达的Bt杀虫蛋白对拟环纹豹蛛的安全性评价[D]. 长沙: 湖南师范大学, 2010.
[58]	白耀宇, 蒋明星, 程家安.转Bt基因水稻对两种弹尾虫及尖钩宽黾蝽捕食作用的影响[J].昆虫学报, 2005, 48(1):42-47. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kcxb200501008
[59]	BAI YY, JIANG M X, CHENG J A, et al.. Effects of Cry1Ab toxin on Propylea japonica(Thunberg)(Coleoptera: Coccinellidae) through its prey, Nilaparvata lugens Stål(Homoptera: Delphacidae), feeding on transgenic Bt rice[J]. Environ Entomol, 2006, 35: 1130-1136. doi: 10.1603/0046-225X-35.4.1130
[60]	安飞. Cry1Ab蛋白在转基因水稻-褐飞虱-黑肩绿盲蝽间的传递路径[D]. 福州: 福建农林大学, 2013.
[61]	田俊策. 杀虫蛋白在Bt水稻-植食者-蜘蛛食物链中的传递规律及对稻田代表性蜘蛛的影响[D]. 杭州: 浙江大学, 2010.
[62]	TIAN J C, LIU Z C, CHEN M, et al. Laboratory and field assessments of prey-mediated effects of transgenic Bt rice on Ummeliata insecticeps(Araneida: Linyphiidae)[J]. Environ Entomol, 2010, 39: 1369-1377. doi: 10.1603/EN10003
[63]	田俊策, 陈洋, 李兆亮, 等.转Cry1Ab基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价[J].中国生物防治学报, 2011, 27(4): 559 -563. http://cdmd.cnki.com.cn/Article/CDMD-10335-2010138851.htm
[64]	王文晶, 王瑞林, 于福兰, 等.转Bt水稻对捕食性天敌黑肩绿盲蝽种群动态的影响[J].应用昆虫学报, 2014, 51(5): 1173-1183. doi: 10.7679/j.issn.2095-1353.2014.139
[65]	LI F F, YE G Y, WU Q, et al. Arthropod abundance and diversity in Bt and non-Bt rice fields[J]. Environ Entomol, 2007, 36: 646-654. doi: 10.1603/0046-225X(2007)36[646:AAADIB]2.0.CO;2
[66]	胡奇勇, 郑宇, 张晓俊, 等.福建省转基因抗虫稻田节肢动物群落多样性研究[J].中国生态农业学报, 2007, 15(4):120-123. http://www.oalib.com/paper/4409599
[67]	刘志诚, 陈洋, 田俊策, 等.转Cry1Ab基因粳稻对稻田节肢动物群落的影响[J].生物安全学报, 2011, 20(1): 69-76. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_swaqxb201101009
[68]	张磊, 朱祯.转基因抗虫水稻对生物多样性的影响[J].遗传, 2011, 33(5): 414-421. http://www.cnki.com.cn/Article/CJFDTOTAL-YCZZ201105002.htm
[69]	徐雪亮, 姚英娟, 陈大洲, 等.转基因抗虫水稻对二化螟幼虫和田间节肢动物群落的影响[J].华中农业大学学报, 2013, 32(5): 50-54. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=hznydx201305009
[70]	SHENG L, LIETTEE V, MIN SHENG Y. Seasonal variability in spider assemblages in traditional and transgenic rice fields[J]. Environmental Entomology, 2016, 45(2): 537-546. doi: 10.1093/ee/nvw002
[71]	崔旭红, 焦晓国, 张国安, 等.转Bt基因水稻对稻飞虱及蜘蛛种群数量的影响[J].华中农业大学学报, 2002, 21(4):356-358. http://www.cnki.com.cn/Article/CJFDTotal-KCZS201405004.htm
[72]	陈睿, 苏军.转基因水稻环境安全研究进展[J].福建农业学报, 2006, 21(4): 384-388. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgdm200702001
[73]	张青玲, 李云河, 华红霞, 等. Bt水稻田重要非靶标节肢动物暴露于Cry2Aa蛋白的程度分析[J].应用生态学报, 2013, 24(6): 1647-1651. http://www.cjae.net/CN/abstract/abstract19104.shtml
[74]	姜永厚, 傅强, 程家安, 等.转Bt基因水稻对二化螟绒茧蜂生物学特性的影响[J].昆虫学报, 2004, 47(1): 124-129. http://www.cnki.com.cn/Article/CJFDTOTAL-KCXB200401018.htm
[75]	李冬虎. 转sck/Cry1Ac双基因抗虫水稻的抗虫评价及对主要非靶标害虫和天敌的影响[D]. 扬州: 扬州大学, 2004.
[76]	刘雨芳, 贺玲, 汪琼, 等.转Cry1Ac/sck基因抗虫水稻对稻田寄生蜂群落影响的评价[J].昆虫学报, 2006, 49(6): 955-962. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=kcxb200606011
[77]	田俊策, 刘志诚, 姚洪渭, 等.转Cry1Ab基因水稻田寄生蜂亚群落结构及其优势类群数量动态的研究[J].环境昆虫学报, 2008, 30(1): 1-7. http://www.cqvip.com/Main/Detail.aspx?id=26825930
[78]	SAXENA D, STOTZKY G. Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ[J]. FEMS Microbiol Ecol, 2000, 33: 35-39. doi: 10.1111/fem.2000.33.issue-1
[79]	白耀宇, 蒋明星, 程家安, 等.转Bt基因水稻Cry1Ab毒蛋白在水稻土中的降解[J].中国水稻科学, 2004, 18(3):255-261. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgsdkx200403014
[80]	白耀宇, 蒋明星, 程家安, 等.转Cry1Ab基因水稻对稻田弹尾虫种群数量的影响[J].应用生态学报, 2006, 17(5):903-906. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yystxb200605029
[81]	WANG HY, YE Q F, GAN J, et al. Biodegradation of Cry1Ab protein from Bt transgenic rice in aerobic and flooded paddy soils[J]. J Agric Food Chem, 2007, 55: 1900-1904. doi: 10.1021/jf062924x
[82]	袁一杨, 戈峰.转Bt基因作物对非靶标土壤动物的影响[J].应用生态学报, 2010, 21(5): 1339-1345. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yystxb201005039
[83]	周学永, 刘宁, 赵曼, 等.转Bt基因作物释放杀虫晶体蛋白对土壤生态安全的影响[J].遗传, 2011, 33(5): 443-448. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=yc201105006
[84]	STOTZKY G. Persistence and biological activity in soil of insecticidal proteins from Bacillus thuringiensis and of bacterial DNA bound on claysand humic acids[J]. J Econ Entomol, 2000, 29: 691-705.
[85]	TAPP H, STOTZKY G. Insecticidal activity of the toxins from Bacillus thuringiensis subspecies kurstaki and tenebrionis adsorbed and boundon pure and soil clays[J]. Appl Environ Microbiol, 1995, 61: 1786-1790. http://cn.bing.com/academic/profile?id=823d5c9f20d1f31b94429590b8bf69d2&encoded=0&v=paper_preview&mkt=zh-cn
[86]	BAI YY, YAN R H, YE G Y, et al. Effects of transgenic rice expressing Bacillus thuringiensis Cry1Ab proteinon ground-dwelling Collembolan community in postharvest seasons[J]. Environ Entomol, 2010, 39: 243-251. doi: 10.1603/EN09149
[87]	祝向钰, 李志毅, 常亮, 等.转Bt水稻土壤跳虫群落组成及其数量变化[J].生态学报, 2012, 32(11): 3546-3554. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=stxb201211027
[88]	吴刚, 李俊生, 肖能文, 等.转Bt基因水稻对土壤跳虫、线虫和螨类种群数量的影响[J].湖北植保, 2012, (5): 6-9. http://mall.cnki.net/magazine/Article/HBZB201205003.htm
[89]	YUAN Y, XIAO N, KROGH P H, et al. Laboratory assessment of the impacts of transgenic Bt rice on the ecological fitness of the soil non-target arthropod, Folsomia candida(Collembola: Isotomidae)[J]. Transgenic Res, 2013, 22(4): 791-803. doi: 10.1007/s11248-013-9687-6
[90]	杨玺, 袁一杨, 戈峰, 等.转Bt基因水稻对白符跳虫适应低温环境与排泄的影响[J].应用昆虫学报, 2014, 51(5): 1204-1214. doi: 10.7679/j.issn.2095-1353.2014.143
[91]	杨玺, 袁一杨, 戈峰, 等. Bt基因水稻对白符跳虫取食选择行为的影响[J].应用昆虫学报, 2014, 51(5): 1197-1203. doi: 10.7679/j.issn.2095-1353.2014.142
[92]	左娇, 郭运玲, 孔华, 等.转基因水稻环境安全评价研究进展[J].热带作物学报, 2013, 34(12): 2521-2526. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_swaqxb201404001
[93]	温李, 鞠颖, 仝泽方, 等.转基因水稻的研究现状及展望[J].贵州农业科学, 2017, 45(6): 21-24. http://mall.cnki.net/magazine/Article/NYZW201011017.htm
[94]	苏京平, 闫双勇, 孙林静, 等.我国转基因水稻研究的现状[J].天津农业科学, 2007, 13(4): 7-11. http://cdmd.cnki.com.cn/Article/CDMD-10466-1015734007.htm
[95]	叶兴国, 王艳丽, 丁文静.主要农作物转基因研究现状和展望[J].中国生物工程杂志, 2006, 26(5): 93-100. http://www.cnki.com.cn/Article/CJFDTOTAL-SWGJ200605020.htm
[96]	JAMES C.2012年全球生物技术/转基因作物商业化发展态势[J].中国生物工程杂志, 2013, 33(2):1-8 http://www.cnki.com.cn/Article/CJFDTotal-SWGJ201704001.htm
[97]	JAMES C. 2015年全球生物技术/转基因作物商业化发展态势[J].中国生物工程杂志, 2016, 36(4): 1-11. http://www.cqvip.com/QK/95819A/201604/668635186.html
[98]	沈平, 武玉花, 梁晋刚, 等.转基因作物发展及应用概述[J].中国生物工程杂志, 2017, 37(1): 119-128. http://www.cnki.com.cn/Article/CJFDTOTAL-HDKJ199906008.htm
[99]	杜建中, 郝曜山, 王亦学, 等.中国转基因主粮作物产业化进展、存在问题及对策[J].生物技术进展, 2016, 6(3): 159-168. http://www.cnki.com.cn/Article/CJFDTOTAL-SWJZ201603002.htm
[100]	戴化勇, 苗阳, 吉小燕.中国转基因作物安全监管面临的重点问题与应对策略[J].农业经济问题, 2016, 16(5): 51-58. http://www.cnki.com.cn/Article/CJFDTotal-NJWT201605007.htm
[101]	谭涛.中国转基因作物产业化发展路径与策略[J].农业技术经济, 2014, (1): 22-30. http://www.cnki.com.cn/Article/CJFDTOTAL-NJJY201506010.htm
[102]	逄金辉, 马彩云, 封勇丽, 等.转基因作物生物安全:科学证据[J].中国生物工程杂志, 2016, 36(1): 122-138. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_swgcjz201601017
[103]	王继磊, 刘迪秋, 丁元明, 等. Bt转基因抗虫植物研究进展[J].生物学杂志, 2010, 27(4): 75-78. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=swxzz201004022
[104]	刘志诚, 叶恭银, 胡萃.抗虫转基因水稻和化学杀虫剂对稻田节肢动物群落的影响[J].应用生态学报, 2004, 15(12): 2309-2314. doi: 10.3321/j.issn:1001-9332.2004.12.022