Optimization and Application of SSR-PCR Reaction System for Macadamia

TANG Ying-ying; YANG Xiang-yan; CAI Yuan-bao; LI Mu; ZENG Li-ming; ZHENG Wen-wu; QIU Wen-wu; LI Ji-dong; YE Wei-yan

doi:10.19303/j.issn.1008-0384.2018.02.009

Volume 33 Issue 2

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(2): 154-158

TANG Ying-ying, YANG Xiang-yan, CAI Yuan-bao, LI Mu, ZENG Li-ming, ZHENG Wen-wu, QIU Wen-wu, LI Ji-dong, YE Wei-yan. Optimization and Application of SSR-PCR Reaction System for Macadamia[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2): 154-158. doi: 10.19303/j.issn.1008-0384.2018.02.009

Citation:

TANG Ying-ying, YANG Xiang-yan, CAI Yuan-bao, LI Mu, ZENG Li-ming, ZHENG Wen-wu, QIU Wen-wu, LI Ji-dong, YE Wei-yan. Optimization and Application of SSR-PCR Reaction System for Macadamia[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2): 154-158. doi: 10.19303/j.issn.1008-0384.2018.02.009

Citation:

TANG Ying-ying, YANG Xiang-yan, CAI Yuan-bao, LI Mu, ZENG Li-ming, ZHENG Wen-wu, QIU Wen-wu, LI Ji-dong, YE Wei-yan. Optimization and Application of SSR-PCR Reaction System for Macadamia[J]. Fujian Journal of Agricultural Sciences, 2018, 33(2): 154-158. doi: 10.19303/j.issn.1008-0384.2018.02.009

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Optimization and Application of SSR-PCR Reaction System for Macadamia

doi: 10.19303/j.issn.1008-0384.2018.02.009

1.
Guangxi Subtropical Crops Research Institute, Nanning, Guangxi 530001, China
2.
Horticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China

Received Date: 2017-11-20
Rev Recd Date: 2018-01-20
Publish Date: 2018-02-01

Abstract

Abstract

Five major factors of the SSR-PCR reaction system were optimized for genomic DNA of macadamia (Macadamia spp.) by a single factor design. The volume of optimum reaction system was 20 μL that consisted of 30 mg·L^-1 template DNA, 1.0 U Taq polymerase, 2.5 mmol·L^-1 Mg²⁺, 0.6 μmol·L^-1 primer and 0.3 mmol·L^-1 dNTPs. On 15 germplasms of macadamia using different SSR primers, the system proved to be reliable and stable in the amplification bands with high resolution. Consequently, it seemed adequate for the identification and genetic diversity analysis of macadamia germplasms.
- macadamia,
- SSR-PCR,
- system optimization,
- single factor design

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References(17)

References

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