Fungal Diversity in Rhizosphere Soil of Soybean Fields under Salt-alkali Stress

ZHANG Jian-feng; JI Li; LIN Chao-long; TIAN Lei; KONG Yu-feng; TIAN Chun-jie

doi:10.19303/j.issn.1008-0384.2017.10.016

Volume 32 Issue 10

Dec. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(10): 1130-1134

ZHANG Jian-feng, JI Li, LIN Chao-long, TIAN Lei, KONG Yu-feng, TIAN Chun-jie. Fungal Diversity in Rhizosphere Soil of Soybean Fields under Salt-alkali Stress[J]. Fujian Journal of Agricultural Sciences, 2017, 32(10): 1130-1134. doi: 10.19303/j.issn.1008-0384.2017.10.016

Citation:

ZHANG Jian-feng, JI Li, LIN Chao-long, TIAN Lei, KONG Yu-feng, TIAN Chun-jie. Fungal Diversity in Rhizosphere Soil of Soybean Fields under Salt-alkali Stress[J]. Fujian Journal of Agricultural Sciences, 2017, 32(10): 1130-1134. doi: 10.19303/j.issn.1008-0384.2017.10.016

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Fungal Diversity in Rhizosphere Soil of Soybean Fields under Salt-alkali Stress

doi: 10.19303/j.issn.1008-0384.2017.10.016

ZHANG Jian-feng^{1, 2
,},
JI Li^{1, 2},
LIN Chao-long¹,
TIAN Lei²,
KONG Yu-feng²,
TIAN Chun-jie^{2
,
,}

1.
College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China
2.
Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin 130102, China

Received Date: 2017-05-20
Rev Recd Date: 2017-07-12
Publish Date: 2017-10-28

Abstract

Abstract

To understand the adaptability of fungi in rhizosphere soil of soybean, two saline-alkali stress ways were set which involved irrigating saline solution (A) and covering saline soil (B), to analyze the diversity of rhizospherical fungi of wild soybean and cultivated soybean, throughthe application of DGGE method. The results showed that the saline alkali stress ways had great influence on the similarity of fungi in rhizosphere of soybean. For example, under the same saline alkali stress way and concentration of A₁, the similarity ratio between the rhizospherical fungi of wild soybean and cultivated soybean was 0.70. In addition, appropriate saline concentration can increase the uniformity and Shannon index of soybean rhizospherical fungi, for example, the uniformity of wild soybean at concentration of B₁ was 0.99, and the Shannon index at concentration of A₂ was 1.87. Moreover, the sequencing of DGGE specific bands and the further comparison with gene bank, some comparative advantageous strain were obtained, such as Fusarium oxysporum, Actinomucor elegans, Marcelleina tuberculispora, Marcelleina persoonii, Tofieldiaceae environmental sample clone and Uncultured eukaryote clone etc.
- wild soybean (Glycine soja),
- cultivated soybean (Glycine max),
- rhizospherical fungi,
- DGGE

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References(17)

References

[1]	杨明, 田静, 高玉山, 等.辽河平原盐碱地改良现状及展望[J].辽宁农业科学, 2012, 264(2):51-54. http://www.doc88.com/p-0781462934145.html
[2]	任丽丽, 任春明, 张伟伟等.短期NaCl胁迫对野生大豆和栽培大豆叶片光合作用的影响[J].大豆科学, 2009, 28(2):239-242. http://www.oalib.com/paper/4514225
[3]	孔钰凤, 朱先灿, 张建峰, 等.野生大豆与栽培大豆抗旱性对接种丛枝菌根真菌的响应[J].土壤与作物, 2017, 6(1):25-31. doi: 10.11689/j.issn.2095－2961.2017.01.004
[4]	郑世英, 萧蓓蕾, 金桂芳. NaCl胁迫对野生大豆和栽培大豆叶绿素及光合特性的影响[J].大豆科学, 2013, 32(4):486-489. http://www.wenkuxiazai.com/doc/5c2968ccf01dc281e43af08b-2.html
[5]	薛忠财, 高辉远, 柳洁.野生大豆和栽培大豆光合机构对NaCl胁迫的不同响应[J].生态学报, 2011, 31(11):3101-3109. http://d.wanfangdata.com.cn/Periodical/stxb201111016
[6]	刘萌, 陈鹏, 王洪芹, 等.盐胁迫对野生和栽培大豆幼苗生长和抗氧化酶活性及膜脂过氧化的影响[J].山东农业科学, 2014, 46(5):38-41. http://d.wanfangdata.com.cn/Periodical/shandnykx201405010
[7]	孟庆英, 张春峰, 于忠和, 等.根瘤菌对大豆根际土壤微生物及大豆农艺性状的影响[J].大豆科学, 2012, 31(3):498-500. http://www.oalib.com/paper/4512910
[8]	苗翠苹. 三七根际土壤微生物的群落特征[D]. 昆明: 云南大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10673-1015608502.htm
[9]	李鹏. 小蓬竹根际土壤可培养微生物及其酶活性研究[D]. 贵阳: 贵州大学, 2015. http://cdmd.cnki.com.cn/Article/CDMD-10657-1015911291.htm
[10]	吴凤芝, 包静, 刘淑芹.盐胁迫对黄瓜根际土壤细菌群落结构和生长发育的影响[J].园艺学报, 2010, 37(5):741-748. doi: 10.1017-S0022112007008002/
[11]	SHI D C, WANG D L. Effects of various salt-alkaline mixed stresses on Aneurolepidium chinense (Trin.) Kitag[J]. Plant and Soil, 2005, 271(1-2):15-26. doi: 10.1007/s11104-004-1307-z
[12]	ZHU M X, GA0 X Y, SHAO X W et al.Effect of different concentrations of saline-alkali stress on growth and yield of rice[J]. Journal of Jilin Agricultural Sciences, 2014, 39(6):12-16. http://or.nsfc.gov.cn/bitstream/00001903-5/236426/1/1000006846810.pdf
[13]	张建峰, 周奇, 田磊, 等.时空因素对火山优势树种根际真菌群落多样性的影响[J].东北林业大学学报, 2016, 44(9):86-92. http://d.wanfangdata.com.cn/Periodical/dblydxxb201609019
[14]	崔红娟, 束长龙, 宋福平, 等.转cry1Ah基因玉米对根际土壤微生物群落结构的影响[J].东北农业大学学报, 2011, 42(7):30-38. http://d.wanfangdata.com.cn/Periodical/dbnydxxb201107006
[15]	吕恒, 牛永春, 邓晖, 等.根际真菌对黄瓜土传病害的抑制作用[J].应用生态学报, 2015, 26(12):3759-3765. http://www.cqvip.com/QK/90626A/201512/666931616.html
[16]	乔蓬蕾. 连作对黄瓜根际土壤微生物菌群的影响[D]. 哈尔滨: 东北农业大学, 2014. http://cdmd.cnki.com.cn/Article/CDMD-10224-1014346907.htm
[17]	陈波, 张锡炎, 黄霄.香蕉枯萎病区土壤真菌多样性分析[J].江苏农业科学, 2013, 41(11):354-357. doi: 10.3969/j.issn.1002-1302.2013.11.138