Genetic Comparisons Between <i>Lactuca pulchella</i> (Pursh) DC and Its Closely Related Lettuce Species

LIN Ling; YU Yun; CHEN Zhi-ming; WANG De-ling; Shen Jian-guo; Fu Jian-guo

doi:10.19303/j.issn.1008-0384.2017.10.006

Volume 32 Issue 10

Dec. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(10): 1077-1081

LIN Ling, YU Yun, CHEN Zhi-ming, WANG De-ling, Shen Jian-guo, Fu Jian-guo. Genetic Comparisons Between Lactuca pulchella (Pursh) DC and Its Closely Related Lettuce Species[J]. Fujian Journal of Agricultural Sciences, 2017, 32(10): 1077-1081. doi: 10.19303/j.issn.1008-0384.2017.10.006

Citation:

LIN Ling, YU Yun, CHEN Zhi-ming, WANG De-ling, Shen Jian-guo, Fu Jian-guo. Genetic Comparisons Between Lactuca pulchella (Pursh) DC and Its Closely Related Lettuce Species[J]. Fujian Journal of Agricultural Sciences, 2017, 32(10): 1077-1081. doi: 10.19303/j.issn.1008-0384.2017.10.006

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Genetic Comparisons Between Lactuca pulchella (Pursh) DC and Its Closely Related Lettuce Species

doi: 10.19303/j.issn.1008-0384.2017.10.006

LIN Ling^{1, 2
,},
YU Yun^{2
,
,},
CHEN Zhi-ming³,
WANG De-ling⁴,
Shen Jian-guo²,
Fu Jian-guo⁵

1.
Quanzhou Entry-Exit Inspection and Quarantine Bureau, Quanzhou, Fujian 362000, China
2.
Inspection & Quarantine Technology Center, Fujian Entry-Exit Inspection and Quarantine Bureau/Fujian Key laboratory for Technology Research of Inspection and Quarantine, Fuzhou, Fujian 350001, China
3.
Fuzhou Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350015, China
4.
Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
5.
Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing Jiangsu 210000, China

Received Date: 2017-07-16
Rev Recd Date: 2017-09-08
Publish Date: 2017-10-28

Abstract

Abstract

The genetic similarity coefficients and cluster analysis maps on Lactuca pulchella (Lp) and its 11 closely related species were obtained by using PCR to amplify ITS, matK and rbcL gene from the total DNA fragments of the lettuce varieties. Based on the ITS sequences, a similarity between Lp and L. sativa var. ramose was found to be 93.6%; between Lp and L. sativa , 93.1%; between Lp and L. serriola , 92.6%;and, between Lp and Mulgedium tataricum , the lowest at 87.8%. According to the matK sequences, a similarity between Lp and L. sativa var. ramose was shown to be 99.2%;between Lp and L. sativa , 99.0%, ; between Lp and L. serriol , 99.1%; and, between Lp and M.tataricum , 98.3%. It appeared that Lp was related closer to the Lactuca species than Mulgedium . By comparing the rbcL , Lp and L. sativa L. var. ramosa had a perfect match with a 100% similarity; whereas, Lp and L. sativa , L. indica , L.serriola , L. hirsute or L. perennis , at 99.7%;and, Lp and M. tataricum , at 99.4%. Consequently, it was concluded that Lp belonged to the genus Lactuca .
- Lactuca pulchella,
- closely related species,
- genetic relationship

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References(18)

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