Screening <i>Bacillus</i> Strains from High-altitude Regions in Search for Identifiable Biological Agents to Control Root Rot Disease in Plants

ZHENG Mei-xia; ZHU Yu-jing; LIU Bo; CHEN Zheng; HUANG Su-fang; LIU Xin

doi:10.19303/j.issn.1008-0384.2017.09.014

Volume 32 Issue 9

Nov. 2017

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(9): 996-1000

ZHENG Mei-xia, ZHU Yu-jing, LIU Bo, CHEN Zheng, HUANG Su-fang, LIU Xin. Screening Bacillus Strains from High-altitude Regions in Search for Identifiable Biological Agents to Control Root Rot Disease in Plants[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 996-1000. doi: 10.19303/j.issn.1008-0384.2017.09.014

Citation:

ZHENG Mei-xia, ZHU Yu-jing, LIU Bo, CHEN Zheng, HUANG Su-fang, LIU Xin. Screening Bacillus Strains from High-altitude Regions in Search for Identifiable Biological Agents to Control Root Rot Disease in Plants[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 996-1000. doi: 10.19303/j.issn.1008-0384.2017.09.014

Citation:

PDF( 1251 KB)

Screening Bacillus Strains from High-altitude Regions in Search for Identifiable Biological Agents to Control Root Rot Disease in Plants

doi: 10.19303/j.issn.1008-0384.2017.09.014

Agricultural Bio-resources Research Institute, Fujian academy of agricultural sciences, Fuzhou, Fujian 350003 China

Received Date: 2017-02-17
Rev Recd Date: 2017-05-04
Publish Date: 2017-09-28

Abstract

Abstract

In search of effective biocontrol species with antagonistic activities against root rot disease in plants, 82 strains of Bacillus bacteria from the high-altitude regions in China were screened. The initial screening was performed on PDA agar plates, and an Oxford cup method was applied to determine the possibly existing antibacterial activities. In the end, FJAT-19708 and FJAT-20265 showed the strongest activities against the root rot. And, their 16S rRNA gene sequences were found homologous to that of Bacillus subtilism.
- Bio-controlling strain,
- Bacillus subtilism,
- root rot

FullText(HTML)

References(27)

References

[1]	SAJANN S, JAMADAR M M. In vitro evaluation of fungicides, biocontrol agents and botanicals for their bioefficacy against root rot of acid lime (Citrus aurantifolia Swingle) in northern Karnataka[J]. Biotechnology Progress, 2016, 18(3):530-537.
[2]	HEMMATI R. Evaluation of trichoderma isolates for biological control of rhizoctonia root rot of bean in Zanjan[J]. Journal of Plant Protection, 2016, 29(4):471-480.
[3]	FERNANDEZ M R, FOX S L, HUCL P, et al. Root rot severity and fungal populations in spring common, durum and spelt wheat, and Kamut grown under organic management in western Canada[J]. Canadian Journal of Plant Science, 2017, 94(5):937-946.
[4]	HAMZA A, MOHAMED A, HAMED S. New trends for biological and non-biological control of tomato root rot, caused by Fusarium solani, under greenhouse conditions[J]. Egyptian Journal of Biological Pest Control, 2016, 26(1):89-96.
[5]	PEREG L L. Black root rot of cotton in Australia:the host, the pathogen and disease management[J]. Crop and Pasture Science, 2017, 64(11-12):1112-1126.
[6]	汪雪静, 卜春亚, 靳永胜, 等.草莓根腐病菌拮抗细菌的分离与鉴定[J].园艺学报, 2011, 38(9):1657-1666. http://d.wanfangdata.com.cn/Periodical/yyxb201109004
[7]	刘丽云, 刘晓林, 刘志恒, 等.辣椒根腐病菌生物学特性研究[J].沈阳农业大学学报, 2007, 38(1):54-58. http://d.wanfangdata.com.cn/Periodical/synydxxb200701011
[8]	WANG L Y, XU L B, HE Y, et al. Separation and screening of biocontrol actinomyces against alfalfa root rot[J]. Plant Diseases and Pests, 2010, 1(2):10-37.
[9]	GV C A W T, DER B A V, DER D K V, et al. Biocontrol by phenazine-1-carboxamide-producing Pseudomonas chlororaphis PCL1391 of tomato root rot caused by Fusarium oxysporumf.sp. radicis-lycopersici[J]. Molecular Plant-Microbe Interactions, 1998, 11(11):1069-1077. doi: 10.1094/MPMI.1998.11.11.1069
[10]	ZHANG S M. Isolation and characterization of antifungal endophytic bacteria from soybean[J]. Microbiology, 2008, 35(10):1593-1599.
[11]	KHALEQUZZAMAN K M. Control of foot and root rot of lentil by using different management tools[J]. Journal of Advanced Research, 2016, 5(1):35-42.
[12]	XIA P, GUO H, ZHAO H, et al. Optimal fertilizer application for panax notoginseng and effect of soil water on root rot disease and saponin contents[J]. Journal of Ginseng Research, 2016, 6(1):38-46.
[13]	顾鑫, 于铭, 丁俊杰, 等.施用长效缓释尿素对大豆根腐病及产量的影响[J].农林学报, 2016, 6(2):44-47. http://d.wanfangdata.com.cn/Periodical/zgncxkkj201602007
[14]	李琼芳, 曾华兰, 叶鹏盛, 等.哈茨木霉(Trichoerma harzianum)T23生防菌筛选及防治中药材根腐病的研究[J].西南大学学报:自然科学版, 2007, 29(11):119-122.
[15]	陈红彩, 游杏, 丁当, 等.白掌根腐病生防菌的筛选与鉴定[J].广东农业科学, 2016, 43(1):89-92. http://d.wanfangdata.com.cn/Periodical/gdnykx201601017
[16]	台莲梅, 郭永霞, 张亚玲, 等.木霉生防菌对大豆幼苗的促生作用及对根腐病的防治效果[J].安徽农业科学, 2013, 41(11):4820-4821. doi: 10.3969/j.issn.0517-6611.2013.11.044
[17]	FAYED S, GHAZAL M, MOSTAFA A, et al. Biochemical actvity of domestic isolate of Bacillus mycoides against some root rot fungi of phasoleous volgaris[J]. Journal of Biological Chemistry, 2013, 8(3):297-317.
[18]	葛慈斌, 刘波, 蓝江林, 等.生防菌JK-2对尖孢镰刀菌抑制特性的研究[J].福建农业学报, 2009, 24(1):29-34. http://www.fjnyxb.cn/CN/abstract/abstract308.shtml
[19]	PHAE C G, SHODA M, KITA N, et al. Biological control of crown and root rot and bacterial wilt of tomato by Bacillus subtilis NB22[J]. Japanese Journal of Phytopathology, 1992, 58(3):329-339. doi: 10.3186/jjphytopath.58.329
[20]	ESTEVEZ D J C, PERCICH J A, GRAHAM P H. Integrated management strategies of bean root rot with Bacillus subtilis and Rhizobium in Minnesota[J]. Field Crops Research, 2002, 74(2-3):107-115. doi: 10.1016/S0378-4290(01)00200-3
[21]	郑雪芳, 刘波, 朱育菁, 等.番茄青枯病生防芽胞杆菌的筛选与鉴定[J].中国生物防治学报, 2016, 32(5):657-665. http://d.wanfangdata.com.cn/Periodical/zgswfz201605016
[22]	孔建, 王文夕, 赵白鸽, 等.枯草芽孢杆菌B-903菌株的研究Ⅰ.对植物病原菌的抑制作用和防治试验[J].中国生物防治学报, 1999, 15(4):157-161. http://d.wanfangdata.com.cn/Periodical/zgswfz199904004
[23]	晏立英, 周乐聪, 谈宇俊, 等.油菜菌核病拮抗细菌的筛选和高效菌株的鉴定[J].中国油料作物学报, 2005, 27(2):55-57. http://d.wanfangdata.com.cn/Periodical/zgylzwxb200502014
[24]	ELLIOTT M L, JARDIN E A D, JR W E B, et al. Viability and stability of biological control agents on cotton and snap bean seeds[J]. Pest Management Science, 2001, 57(8):695-706. doi: 10.1002/(ISSN)1526-4998
[25]	LI Y, ZHANG L, WANG C, et al. Antagonistic mechanism and control effect of Bacillus subtilis Y2 against Fusarium oxysporum causing soybean root rot[J].African Journal of Microbiology Research, 2013, 7(8):652-656.
[26]	LI Y G, MA F M. Antagonistic mechanism of Fusarium oxysporum of soybean root rot by Bacillus subtilis[J]. Applied Mechanics and Materials, 2011, 108(3):127-131.
[27]	KITA N, OHYA T, UEKUSA H, et al. Biological control of damping-off of tomato seedlings and cucumber phomopsis root rot by Bacillus subtilis RB14-C[J]. Japan Agricultural Research Quarterly, 2005, 39(2):109-114. doi: 10.6090/jarq.39.109