Cloning and Sequence Analysis of <i>OsSDR</i>1 Gene from Restorer Line of Hybrid Rice Fuihui 7185

XU Hui-Bin; XU Qian; LIAN Ling; ZHU Yong-sheng; JIANG Jia-huan; XIE Hong-guang; LUO Xi; JIANG Min-rong; ZHENG Yan-mei; XIE Hua-an; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2017.09.004

Volume 32 Issue 9

Nov. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(9): 939-945

XU Hui-Bin, XU Qian, LIAN Ling, ZHU Yong-sheng, JIANG Jia-huan, XIE Hong-guang, LUO Xi, JIANG Min-rong, ZHENG Yan-mei, XIE Hua-an, ZHANG Jian-fu. Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 939-945. doi: 10.19303/j.issn.1008-0384.2017.09.004

Citation:

XU Hui-Bin, XU Qian, LIAN Ling, ZHU Yong-sheng, JIANG Jia-huan, XIE Hong-guang, LUO Xi, JIANG Min-rong, ZHENG Yan-mei, XIE Hua-an, ZHANG Jian-fu. Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 939-945. doi: 10.19303/j.issn.1008-0384.2017.09.004

Citation:

XU Hui-Bin, XU Qian, LIAN Ling, ZHU Yong-sheng, JIANG Jia-huan, XIE Hong-guang, LUO Xi, JIANG Min-rong, ZHENG Yan-mei, XIE Hua-an, ZHANG Jian-fu. Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 939-945. doi: 10.19303/j.issn.1008-0384.2017.09.004

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Cloning and Sequence Analysis of OsSDR1 Gene from Restorer Line of Hybrid Rice Fuihui 7185

doi: 10.19303/j.issn.1008-0384.2017.09.004

XU Hui-Bin^{1, 2
,},
XU Qian^{1, 2, 3},
LIAN Ling^{1, 2},
ZHU Yong-sheng^{1, 2, 3},
JIANG Jia-huan^{1, 2},
XIE Hong-guang^{1, 2},
LUO Xi^{1, 2, 3},
JIANG Min-rong^{1, 2},
ZHENG Yan-mei^{1, 2, 3},
XIE Hua-an^{1, 2, 3
,
,},
ZHANG Jian-fu^{1, 2, 3
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Incubator of National key Laboratory of Crops Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Science and Technology/South China Base of National Key Laboratory of Hybrid rice for China/National Engineering Laboratory of Rice of China, Fuzhou, Fujian 350003, China
3.
Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China

Received Date: 2017-04-12
Rev Recd Date: 2017-07-14
Publish Date: 2017-09-28

Abstract

Abstract

The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants. Hormones play an important regulatory role on it, While, short-chain dehydrogenase/reductase (SDR1) is a molecular link between nutrient signaling and plant hormone biosynthesis. The coding sequences (CDS) of SDR1 were cloned from different dormancy varieties FH7185, FH653 and YH290, respectively. The results of sequences blast indicated that the coding regions of OsSDR1 among the three varieties were homologous, but there were 9 bases deletion, coding three alanine compared to Nipponbare. Meanwhile, the results of real-time quantitative PCR (qRT-PCR)of SDR1 expression level in seed embryo among different dormancy varieties indicated that the expression level of SDR1 was significantly different among them. Moreover, the corresponding interaction proteins of OsSDR1 were screened by GST pull-down experiments and an interaction protein of OsSDR1 was found and its Genbank serial number designated as Os05g0140800, it was verified related to seed dormancy. The preliminary results showed that SDR1 gene might regulate seed dormancy through interaction with other structural elements.
- rice,
- Fuhui 7185,
- OsSDR1,
- cloning,
- sequence analysis,
- interaction protein

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References(22)

References

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