Full-length cDNA Library and EST Analysis on <i>Pesudocohnilembus persalinus</i>

LIN Neng-feng; ZENG Hong

doi:10.19303/j.issn.1008-0384.2017.09.001

Volume 32 Issue 9

Nov. 2017

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LIN Neng-feng, ZENG Hong. Full-length cDNA Library and EST Analysis on Pesudocohnilembus persalinus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 921-925. doi: 10.19303/j.issn.1008-0384.2017.09.001

Citation:

LIN Neng-feng, ZENG Hong. Full-length cDNA Library and EST Analysis on Pesudocohnilembus persalinus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(9): 921-925. doi: 10.19303/j.issn.1008-0384.2017.09.001

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Full-length cDNA Library and EST Analysis on Pesudocohnilembus persalinus

doi: 10.19303/j.issn.1008-0384.2017.09.001

LIN Neng-feng^1
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ZENG Hong^{2
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1.
Institute of Biotechnology, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350003, China
2.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China

Received Date: 2017-05-23
Rev Recd Date: 2017-07-05
Publish Date: 2017-09-28

Abstract

Abstract

Pesudocohnilembus persalinus is a harmful parasite found in marine fish. Using SMART technology, a full-length cDNA library of P. persalinus was constructed. Titer of the primary library was 3.3×10⁶ cfu·mL^-1, and the library capacity was 1.5×10⁷ cfu with a recombination rate of 97%. A PCR amplification on 24 random clones revealed that the inserted cDNA fragments ranged from 300-2 000 bp, with an average length over 500 bp. Subsequently, 1 200 clones were selected from the library for EST sequencing. As a result, 1 032 high-quality ESTs were obtained and assembled into 292 unigenes including 102 contigs and 190 unique ESTs with a redundancy rate of 71.7%. In a blast analysis against NCBI database, 215 of the unigenes were homologous to the corresponding genes of ciliates with free-living and parasitic lifestyle, such as Tetrahymena thermophila, Paramecium tetraurelia, Ichthyophthirius multifiliis, Cryptocaryon irritans, Oxytricha trifallax, etc. But, only 134 ESTs had been annotated. The remainders were hypothetical protein genes. The results obtained from this study would substantially facilitate the molecular cloning and characterization of the functional genes in P. persalinus.
- Pesudocohnilembus persalinus,
- cDNA library,
- EST sequencing

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References(17)

References

[1]	杜佳垠.鱼类盾纤虫病危害状况与研究进展[J].北京水产, 2007, 26(6):25-33. http://d.wanfangdata.com.cn/Periodical/bjsc200706011
[2]	杜佳垠.海水养殖鱼类盾纤虫病[J].渔业现代化, 2001, 29(3):12-14. http://d.wanfangdata.com.cn/Periodical/yyxdh200103004
[3]	KIM S M, CHO J B, LEE E H, et al. Pseudocohnilembus persalinus is an additional species causing scuticociliatosis in olive flounder Paralichthys olivaceus[J]. Dis Aquat Org, 2004, 62:239-244. doi: 10.3354/dao062239
[4]	SONG J Y, KITAMURA S, Oh M J, et al. Pathogenicity of Miamiensis avidus (syn. Philasterides dicentrarchi), Pseudocohnilembus persalinus, Pseudocohnilembus hargisi and Uronema marinum (Ciliophora, Scuticociliatida)[J]. Dis Aquat Org, 2009, 83:133-143. doi: 10.3354/dao02017
[5]	张立坤, 王玉梅, 肖国华, 等.寄生于养殖牙鲆体表溃烂组织中的水滴伪康纤虫[J].河北渔业, 2007, 35(10):42-43. doi: 10.3969/j.issn.1004-6755.2007.10.021
[6]	潘旭明, 马洪钢, 邵晨, 等.海洋纤毛虫——水滴伪康纤虫的口器发生及形态学重描述[J].水生生物学报, 2012, 36(3):489-495. http://www.cnki.com.cn/Article/CJFDTOTAL-SSWX201203014.htm
[7]	HAMPTON J R, SCHWARTZ J R L. Contractile vacuole function in Pseudocohnilembus persalinus[J]. Comp Biochem Physiol, 1976, 55:1-4. doi: 10.1016/0300-9629(76)90113-4
[8]	JESSE C, THOMPSON J R. Pseudocohnilembus marinus a Hymenostome ciliate from the Virginia coast[J]. J Protozool, 1966, 13(3):463-465. doi: 10.1111/jeu.1966.13.issue-3
[9]	GAO F, STRUDER-KYPKE M, YI Z, et al. Phylogenetic analysis and taxonomic distinction of six genera of pathogenic scuticociliates (Protozoa, Ciliophora) inferred from small-subunit rRNA gene sequences[J]. Intern J System Evol Microb, 2012, 62:246-256. doi: 10.1099/ijs.0.028464-0
[10]	张立坤, 赵振良, 王玉梅, 等. 3种环境因子对致病性纤毛虫——贪食迈阿密虫种群生长的影响[J].海洋水产研究, 2008, 29(6):122-125. http://d.wanfangdata.com.cn/Periodical/hyscyj200806019
[11]	曾红, 沈琳琳, 林能锋, 等.不同培养条件对海洋尾丝虫种群增长的影响[J].福建师范大学学报:自然科学版, 2015, 31(1):87-92.
[12]	沈琳琳, 林能锋, 詹子锋, 等.不同培养条件水滴伪康纤虫种群生长研究[J].福建农业学报, 2014;29(2):107-112. http://www.fjnyxb.cn/CN/abstract/abstract2361.shtml
[13]	宋微波, 赵元莙, 徐奎栋, 等.海水养殖中的危害性原生动物[M].北京:科学出版社, 2003.
[14]	XIONG J, WANG G, CHENG J, et al. Genome of the facultative scuticociliatosis pathogen Pseudocohnilembus persalinus provides insight into its virulence through horizontal gene transfer[J]. Sci Rep, 2015, (5):15470.
[15]	MOUSTAFA E M M, NAOTA M, MORITA T, et al. Pathological Study on the Scuticociliatosis Affecting Farmed Japanese Flounder[J]. J Vet Med Sci, 2010, 72(10):1359-1362. doi: 10.1292/jvms.10-0026
[16]	JIN C N, HARIKRISHNAN R, MOON Y G, et al. Histopathological changes of Korea cultured olive flounder, Paralichthys olivaceus due to scuticociliatosis caused by histophagous scuticociliate, Philasterides dicentrarachi[J]. Vet Parasit, 2009, 161:292-301. doi: 10.1016/j.vetpar.2009.01.033
[17]	PUIG L, TRAVESET R, PALENZUELA O. Histopathology of experimental scuticociliatosis in turbot Scophthalmus maximus[J]. Dis Aquat Org, 2007, 76:131-140. doi: 10.3354/dao076131