Optimization of Flavonoids Extraction from Leaves of <i>Helianthus tuberosus</i> L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract

HE Shu-lan; ZHU Fu-rong; ZHU Hong-yang; RAO Xue-e; LUO Wen-fang

doi:10.19303/j.issn.1008-0384.2017.08.017

Volume 32 Issue 8

Oct. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(8): 897-904

HE Shu-lan, ZHU Fu-rong, ZHU Hong-yang, RAO Xue-e, LUO Wen-fang. Optimization of Flavonoids Extraction from Leaves of Helianthus tuberosus L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 897-904. doi: 10.19303/j.issn.1008-0384.2017.08.017

Citation:

HE Shu-lan, ZHU Fu-rong, ZHU Hong-yang, RAO Xue-e, LUO Wen-fang. Optimization of Flavonoids Extraction from Leaves of Helianthus tuberosus L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 897-904. doi: 10.19303/j.issn.1008-0384.2017.08.017

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Optimization of Flavonoids Extraction from Leaves of Helianthus tuberosus L. by Response Surface Methodology and Determination of Antimicrobial Activity of Resulting Extract

doi: 10.19303/j.issn.1008-0384.2017.08.017

Fujian Health College, Fuzhou, Fujian 350101, China

Received Date: 2017-04-15
Rev Recd Date: 2017-06-10
Publish Date: 2017-08-28

Abstract

Abstract

The ultrasound-assisted extraction of flavonoids from leaves of Helianthus tuberosus L. was optimized. The extract obtained was evaluated for its antimicrobial activity.Based on the results from single-factor tests, the extraction conditions were further optimized using the response surface methodology. It was found that when 40% ethanol was applied as the solvent in a substrate:solvent ratio of 1:40 to extract the leaves for 20 min at 64℃, the yield and efficiency of the process could be maximized to reach a flavonoid extraction rate of 1.47 mg·g^-1. The flavonoids obtained showed an excellent antibacterial activity on Escherichiacoli, but not on Staphylococcus aureus or Bacillus subtilis.
- response surface methodology,
- Helianthus tuberosus L.,
- flavonoids,
- antimicrobial activity

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References(12)

References

[1]	中国植物志编辑委员会.中国植物志[M].北京:科学出版社, 1980:359.
[2]	刘海伟, 刘兆普, 刘玲, 等.菊芋叶片提取物抑菌活性与化学成分的研究[J].天然产物研究与开发, 2007, 19(3):405-409. http://www.cnki.com.cn/Article/CJFDTOTAL-TRCW200703010.htm
[3]	张海娟, 刘玲, 郑晓涛, 等.菊芋叶片绿原酸的提取工艺条件优化研究[J].食品工业科技, 2011, 32(5):261-262, 265. http://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201105058.htm
[4]	JAISWAL RAKESH, DESHPANDE SAGAR, KUHNERT NIKOLAI. Profiling the chlorogenic acids of Rudbeckia hirta, Helianthus tuberosus, Carlina acaulis and Symphyotrichum novae-angliae leaves by LC-MS(n).[J]. Phytochemical Analysis, 2011, 22(5):432-441. doi: 10.1002/pca.v22.5
[5]	延玺, 刘会青, 邹永青, 等.黄酮类化合物生理活性及合成研究进展[J].有机化学, 2008, 28(9):1534. http://www.cnki.com.cn/Article/CJFDTOTAL-YJHU200809006.htm
[6]	NASIR TAJUDDEEN, MUHAMMAD SANI SALLAU, ALIYU MUHAMMAD MUSA, et al. Flavonoids with antimicrobial activity from the stem bark of Commiphora pedunculata (Kotschy & amp; Peyr.) Engl.[J].Natural Product Research, 2014, 28(21):1915. doi: 10.1080/14786419.2014.947488
[7]	ZOU Z X, XU P S, WU C R, et al. Carboxymethyl flavonoids and a chromone with antimicrobial activity from Selaginella moellendorffii Hieron[J]. Fitoterapia, 2016, 111:124. doi: 10.1016/j.fitote.2016.04.022
[8]	刘玲玲, 孔涛, 王海英, 等.菊芋总黄酮提取工艺研究[J].山西农业科学, 2008, (09):62-65. http://www.cnki.com.cn/Article/CJFDTOTAL-TRCW201211030.htm
[9]	郑晓涛, 隆小华, 刘玲, 等.菊芋叶总黄酮提取工艺优化及含量动态变化[J].天然产物研究与开发, 2012, 24(11):1642-1645, 1689. http://www.cnki.com.cn/Article/CJFDTOTAL-TRCW201211030.htm
[10]	杨明俊, 王亮, 吴婧, 等.菊芋叶黄酮类化合物的体外抗氧化活性研究[J].贵州农业科学, 2011, 39(4):52-54. http://www.cnki.com.cn/Article/CJFDTOTAL-GATE201104019.htm
[11]	李淑红, 王京仁, 成钢, 等.12种中草药对金黄色葡萄糖球菌体外抑菌作用的研究[J].黑龙江畜牧兽医, 2014, (13):148-149.
[12]	吴婧. 菊芋叶黄酮类化合物的提取及其抗氧化性、抗肿瘤和抑菌性的研究[D]. 兰州: 兰州理工大学, 2010. http://cdmd.cnki.com.cn/Article/CDMD-10731-1011134194.htm