Differentiation between Reciprocal-crossed Hybrids of Dendrobium officinale and D.huoshanense Using SSR Markers

LI Yong-qing; JIANG Jin-lan; YE Wei; CHAO Yi-yang; LEI Fu-gui; YANG Xue

doi:10.19303/j.issn.1008-0384.2017.08.012

Volume 32 Issue 8

Oct. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(8): 870-873

LI Yong-qing, JIANG Jin-lan, YE Wei, CHAO Yi-yang, LEI Fu-gui, YANG Xue. Differentiation between Reciprocal-crossed Hybrids of Dendrobium officinale and D.huoshanense Using SSR Markers[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 870-873. doi: 10.19303/j.issn.1008-0384.2017.08.012

Citation:

LI Yong-qing, JIANG Jin-lan, YE Wei, CHAO Yi-yang, LEI Fu-gui, YANG Xue. Differentiation between Reciprocal-crossed Hybrids of Dendrobium officinale and D.huoshanense Using SSR Markers[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 870-873. doi: 10.19303/j.issn.1008-0384.2017.08.012

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Differentiation between Reciprocal-crossed Hybrids of Dendrobium officinale and D.huoshanense Using SSR Markers

doi: 10.19303/j.issn.1008-0384.2017.08.012

Sanming Academy of Agricultural Sciences, shaaxian, Fujian 36500, China

Received Date: 2017-05-09
Rev Recd Date: 2017-08-07
Publish Date: 2017-08-28

Abstract

Abstract

The authenticity of hybrids created by reciprocal crossing between Dendrobium officinale and D. huoshanense was not always apparent. To resolve the confusion, this study applied SSR markers to differentiate them among 63 F1 generation of the hybrids. Three out of 21 pairs of the SSR primers were found to have polymorphic bands, i.e., 14.3% of all, in the parental lines. By using these 3 pairs of primers, 25 out of 32 known (D. officinale×D. huoshanense) hybrids were correctly identified rendering a positive identification rate of 78.1%; while, 28 out of 31 known (D. huoshanense×D. officinale) hybrids were positively identified with a rate of 90.3%.
- Dendrobium officinale,
- Dendrobium huoshanense,
- reciprocal cross,
- SSR,
- hybrid identification

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References(15)

References

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