Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings

FU Qiu-ling; FU Guang-hua; CHEN Cui-teng; CHENG Long-fei; WAN Chun-he; SHI Shao-hua; CHEN Hong-mei; CHEN Zhen; ZHU Chun-hua; HUANG Yu

doi:10.19303/j.issn.1008-0384.2017.08.001

Volume 32 Issue 8

Oct. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(8): 813-817

FU Qiu-ling, FU Guang-hua, CHEN Cui-teng, CHENG Long-fei, WAN Chun-he, SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, ZHU Chun-hua, HUANG Yu. Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 813-817. doi: 10.19303/j.issn.1008-0384.2017.08.001

Citation:

FU Qiu-ling, FU Guang-hua, CHEN Cui-teng, CHENG Long-fei, WAN Chun-he, SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, ZHU Chun-hua, HUANG Yu. Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 813-817. doi: 10.19303/j.issn.1008-0384.2017.08.001

Citation:

FU Qiu-ling, FU Guang-hua, CHEN Cui-teng, CHENG Long-fei, WAN Chun-he, SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, ZHU Chun-hua, HUANG Yu. Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings[J]. Fujian Journal of Agricultural Sciences, 2017, 32(8): 813-817. doi: 10.19303/j.issn.1008-0384.2017.08.001

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Identification and Sequencing of Duck Hepatitis A Virus 1 Subtype a Isolated from Mule Ducklings

doi: 10.19303/j.issn.1008-0384.2017.08.001

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian 350013, China

Received Date: 2017-06-12
Rev Recd Date: 2017-07-10
Publish Date: 2017-08-28

Abstract

Abstract

A strain of duck hepatitis A virus 1 subtype a (DHAV-1a), coded as FJ1605, was isolated from the mule ducklings with pancreatitis. It was identified by RT-PCR analysis and a neutralization test in duck embryos that showed the strain specifically being neutralized by the anti-DHAV-1a hyperimmune serum. Molecular characteristics of VP1 gene of the virus was analyzed to indicate the nucleotide to be 714 bp in length with a homology with those of other DHAV-1a isolates ranging from 98.1% to 99.7% and sharing the greatest similarity to strain FJ1220.On the other hand, the homology between FJ1605 and DHAV-2 was merely 65.7%; and, that between FJ1605 and DHAV-3, approximately 68.3%. Phylogenetic analysis on the VP1 gene also showed FJ1605 to be in the viral lineage of DHAV-1a. Furthermore, an animal infection test clearly displayed the same clinical symptoms as those shown inclinical cases. Thus, it was concluded that the pancreatitis in the mule ducklings was caused by DHAV-1a.
- DHAV-1a,
- mule duckling,
- VP1 gene

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References(15)

References

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