Rapid PCR Differentiation Between <i>Lobelia chinensis</i> and <i>Mazus pumilus</i>

CHEN Jian-xiong; WEI Yi-cong; HUANFG Ze-hao; XU Hui-long; LU Wei; LIANG Yi-chi

doi:10.19303/j.issn.1008-0384.2017.07.008

Volume 32 Issue 7

Oct. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(7): 730-733

CHEN Jian-xiong, WEI Yi-cong, HUANFG Ze-hao, XU Hui-long, LU Wei, LIANG Yi-chi. Rapid PCR Differentiation Between Lobelia chinensis and Mazus pumilus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 730-733. doi: 10.19303/j.issn.1008-0384.2017.07.008

Citation:

CHEN Jian-xiong, WEI Yi-cong, HUANFG Ze-hao, XU Hui-long, LU Wei, LIANG Yi-chi. Rapid PCR Differentiation Between Lobelia chinensis and Mazus pumilus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(7): 730-733. doi: 10.19303/j.issn.1008-0384.2017.07.008

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Rapid PCR Differentiation Between Lobelia chinensis and Mazus pumilus

doi: 10.19303/j.issn.1008-0384.2017.07.008

College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China

Received Date: 2017-04-07
Rev Recd Date: 2017-06-01
Publish Date: 2017-07-01

Abstract

Abstract

Molecular identifications of Lobelia chinensis and Mazus pumilus, as well as a specific PCR rapid method based on the SNP in rbcL sequence to differentiate between the two rather similar Chinese medicinal material were establish in this study. Commercial samples of 9 L. chinensis and 7 M. pumilus from different origins were collected to extract total DNA for rbcL gene sequencing. SNPs in the rbcL sequences of the samples were isolated using the ClustulX 2.1 program. The primers for identifying the two material were designed based on the SNP site. A specific PCR method was subsequently developed to rapidly and effectively detect the markers by the addition of SYBR Green I dye.
- Lobelia chinensis,
- Mazus pumilus,
- SNP,
- molecular identification

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References(16)

References

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