SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato

LIU Zhong-hua; LIN Zhi-jian; LI Hua-wei; QIU Yong-xiang; QIU Si-xin; ZHANG Hong; YU Hua; LAN Chun-zhun

doi:10.19303/j.issn.1008-0384.2017.06.013

Volume 32 Issue 6

Aug. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(6): 639-644

LIU Zhong-hua, LIN Zhi-jian, LI Hua-wei, QIU Yong-xiang, QIU Si-xin, ZHANG Hong, YU Hua, LAN Chun-zhun. SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 639-644. doi: 10.19303/j.issn.1008-0384.2017.06.013

Citation:

LIU Zhong-hua, LIN Zhi-jian, LI Hua-wei, QIU Yong-xiang, QIU Si-xin, ZHANG Hong, YU Hua, LAN Chun-zhun. SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 639-644. doi: 10.19303/j.issn.1008-0384.2017.06.013

Citation:

LIU Zhong-hua, LIN Zhi-jian, LI Hua-wei, QIU Yong-xiang, QIU Si-xin, ZHANG Hong, YU Hua, LAN Chun-zhun. SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 639-644. doi: 10.19303/j.issn.1008-0384.2017.06.013

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SRAP Marker Associated with Fusarium-wilt Resistance Gene in Sweet Potato

doi: 10.19303/j.issn.1008-0384.2017.06.013

1.
Crop Research Institute, Fujian Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China, Fuzhou, Fujian 350013, China
2.
Central Laboratory, Fujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory of Precision Measurement for Agriculture, Fuzhou, Fujian 350002, China
3.
Fuding Agriculture Bureau, Fuding, Fujian 355200, China

Received Date: 2016-10-05
Rev Recd Date: 2016-11-21
Publish Date: 2017-06-28

Abstract

Abstract

Fusarium wilt (F.W) disease-resistant molecular markers can be used to screen disease-resistant lines in early planting stage of sweet potato, which can also greatly improve the efficiency of breeding for sweet potato disease-resistance.The F1 progenies were obtained from the cross of sweet potato c.v. Jinshan57, which is higly resistant to Fusarium wilt (F.W), and c.v. Xinzhonghua, which is highly susceptible to F.W. After disease-resistant identification, 7 highly disease-resistant and 7 highly disease-susceptible lines were selected from F1 progenies and were used to establish the resistant and susceptible DNA pools. The genomic DNA of the resistant and susceptible pools were PCR-amplified respectively with 276 pairs of SRAP primers, and 98 pairs of primer generated polymorphic bands between the disease-resistant and susceptible pools. Through further screening using a group of small population and their parents, we got a SRAP marker named a12b8, which would be linked to the resistance of F.W. in sweet potato. This marker would be used for identifying the resistance to F.W in the early growth stage of sweet potato and also would be utilized for improving the efficiency of F.W disease-resistant breeding by molecular marker assisted selection for sweet potato.
- sweet potato,
- fusarium wilt,
- sequence-related amplified polymorphism (SRAP),
- molecular markers

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References(12)

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