Isolation, Identification and Fungicide Toxicity of <i>Ustilaginoidea virens</i>

HU Xian-feng; LI Rong-yu; WANG Jian; LI Ming; LI Si-bo; HUANG Yong-hui; WU Shuai; ZHAO Qi-jun

doi:10.19303/j.issn.1008-0384.2017.06.012

Volume 32 Issue 6

Aug. 2017

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(6): 634-638

HU Xian-feng, LI Rong-yu, WANG Jian, LI Ming, LI Si-bo, HUANG Yong-hui, WU Shuai, ZHAO Qi-jun. Isolation, Identification and Fungicide Toxicity of Ustilaginoidea virens[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 634-638. doi: 10.19303/j.issn.1008-0384.2017.06.012

Citation:

HU Xian-feng, LI Rong-yu, WANG Jian, LI Ming, LI Si-bo, HUANG Yong-hui, WU Shuai, ZHAO Qi-jun. Isolation, Identification and Fungicide Toxicity of Ustilaginoidea virens[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 634-638. doi: 10.19303/j.issn.1008-0384.2017.06.012

Citation:

PDF( 1586 KB)

Isolation, Identification and Fungicide Toxicity of Ustilaginoidea virens

doi: 10.19303/j.issn.1008-0384.2017.06.012

HU Xian-feng^{1, 2
,},
LI Rong-yu^{1, 2, 3},
WANG Jian^{1, 2},
LI Ming^{1, 3
,
,},
LI Si-bo⁴,
HUANG Yong-hui^{1, 2},
WU Shuai^{1, 2},
ZHAO Qi-jun^{1, 2}

1.
Institute of Crop Protection, Guizhou University, Guiyang, Guizhou 550025, China
2.
College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, China
3.
The Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region, Guiyang, Guizhou 550025, China
4.
College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning 110161, China

Received Date: 2017-03-22
Rev Recd Date: 2017-05-10
Publish Date: 2017-06-28

Abstract

Abstract

Two methods for isolating HP-7 (Ustilaginoidea virens) and 7 fungicides for controlling the false smut disease on rice caused by the pathogen were compared. Fungal spores at different maturation stages were separated by means of shaking or flame burning. HP-7 was verified by its morphology, pathogenicity, and rDNA-ITS sequences. The sensitivity of HP-7 toward and toxicity on the fungi by the fungicides were determined based on the fungal mycelium growth under treatment. It was found that the successful rate in separating the fresh yellow chiamydospores by shaking was up to 98.33%, which was better than the flaming method. The rDNA-ITS sequences of HP-7 were submitted to NCBI for a BLAST analysis that showed a 99% homogeny with U. virens. Combining that result with the morphological observations, HP-7 was verified to be the pathogen that caused the rice false smut. Among the fungicides tested, tebuconazole and azoxystrobin exhibited the highest inhibition rates on HP-7, with EC₅₀ at 0.109 1 and 0.158 5 mg·L^-1, respectively. It appeared that an effective spore separation method was identified, and the positive identification and appropriate selection on fungicide for effective control of the false smut disease on rice crops were obtained.
- rice false smut,
- Ustilaginoidea virens,
- identification,
- laboratory toxicity test

FullText(HTML)

References(14)

References

[1]	TANAKA E, ASHIZAWA T S R, TANAKA C. Villosiclava virens gen. nov. comb. nov. teleomorph of Ustilaginoidea virens, the causal agent of rice false smut[J]. Mycotaxon, 2008, 106(11-12):491-501. https://www.researchgate.net/publication/234130822_Villosiclava_virens_gen._nov._comb._nov._teleomorph_of_Ustilaginoidea_virens_the_causal_agent_of_rice_false_smut/reviews/28024
[2]	胡东维, 王疏.稻曲病菌侵染机制研究现状与展望[J].中国农业科学, 2012, 45(22):4604-4611. doi: 10.3864/j.issn.0578-1752.2012.22.006
[3]	李丹阳, 邓启得, 雍明丽, 等.稻曲病菌菌核降解微生物的筛选与作用机制分析[J].中国生物防治学报, 2016, 32(2):258-264. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201602019.htm
[4]	王永强, 樊荣辉, 杨炳辉, 等.稻绿核菌(稻曲病菌)分离方法的比较研究[J].菌物学报, 2010, 29(1):59-63. http://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201001011.htm
[5]	徐晗, 白元俊, 缪建锟, 等.不同储存时期稻曲病菌分离方法比较研究[J].吉林农业大学学报, 2017, 39(1):1-5. http://youxian.cnki.com.cn/yxdetail.aspx?filename=JLNY20161109000&dbname=CAPJ2015
[6]	唐爽爽, 刘志恒, 余朝阁, 等. 9种杀菌剂对西瓜炭疽病菌的室内毒力测定及配比试验[J].植物保护, 2014, 40(6):171-175. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH201406035.htm
[7]	林瑜凡. 稻曲病菌分离、培养及接种技术的初步研究[D]. 雅安: 四川农业大学, 2009. http://cdmd.cnki.com.cn/Article/CDMD-10626-2009259204.htm
[8]	张君成, 张炳欣, 陈志谊, 等.稻曲病的接种方法及其效果初探[J].中国水稻科学, 2003, 17(4):390-392. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGSK200304022.htm
[9]	陈吉良, 黄小龙, 吴安迪, 等.一种快速高效提取病原真菌DNA作为PCR模板的方法[J].菌物学报, 2011, 30(1):147-149. http://www.cnki.com.cn/Article/CJFDTOTAL-JWXT201101025.htm
[10]	张亮, 袁争, 朱蔚, 等. 4种植物提取物对茶炭疽病菌的体外抑制作用[J].植物保护, 2012, 38(4):137-140. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH201204033.htm
[11]	陈丽, 胡东维, 陈美军, 等.稻曲球及稻曲病菌菌落微结构的SEM观察[J].菌物学报, 2007, 26(1):89-96. http://www.cnki.com.cn/Article/CJFDTOTAL-JWXT200701015.htm
[12]	刘明霞, 秦姝, 李颖, 等.稻曲病菌分离技术、培养条件研究[J].辽宁农业科学, 2009, 50(6):20-22. http://www.cnki.com.cn/Article/CJFDTOTAL-LNNY200906005.htm
[13]	张君成, 陈志谊, 张炳欣, 等.稻曲病菌的形态学观察研究[J].植物病理学报, 2003, 33(6):517-523. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBL200306007.htm
[14]	钱双宏, 沈林波, 熊国如, 等.甘蔗褐条病病原菌分离鉴定及其室内毒力的测定[J].热带作物学报, 2015, 36(2):353-357. http://www.cnki.com.cn/Article/CJFDTOTAL-RDZX201502022.htm