Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich <i>Agaricus blazei</i>

YANG Shu-yun

doi:10.19303/j.issn.1008-0384.2017.05.013

Volume 32 Issue 5

Jun. 2017

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(5): 531-535

YANG Shu-yun. Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei[J]. Fujian Journal of Agricultural Sciences, 2017, 32(5): 531-535. doi: 10.19303/j.issn.1008-0384.2017.05.013

Citation:

YANG Shu-yun. Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei[J]. Fujian Journal of Agricultural Sciences, 2017, 32(5): 531-535. doi: 10.19303/j.issn.1008-0384.2017.05.013

Citation:

PDF( 1461 KB)

Optimization of Polysaccharide Extraction from Mycelia of Selenium-rich Agaricus blazei

doi: 10.19303/j.issn.1008-0384.2017.05.013

YANG Shu-yun

Edible Fungi Technology Extension Station of Fujian, Fuzhou, Fujian 350003, China

Received Date: 2016-12-21
Rev Recd Date: 2017-02-10
Publish Date: 2017-05-28

Abstract

Abstract

The hot-water extraction of polysaccharides from the mycelia of Agaricus blazeia grown on a selenium-enriched medium was optimized to facilitate product development of health foods with the functional ingredient. A single factor followed by an orthogonal experiment was carried out to show the extraction yield to be significantly affected by the altered processing conditions. The optimized extraction conditions included a sample/water ratio of 1/35 (g·mL^-1), an ethanol concentration for precipitation at 90%, a temperature of 85℃, and 3 repeated cycles that lasted 3.5 h. As a result, a yield on polysaccharides reached 69.5 mg·g^-1; and the content of selenium in polysaccharides reached 12.1 μg·g^-1(dry weight of mycelium). The procedures were simple, stable, practical, and appropriate for the extraction.
- Agaricus blazei Murill,
- selenium-rich mycelium,
- polysaccharides,
- extraction

FullText(HTML)

References(18)

References

[1]	吴法先.硒和富硒食用菌对人体健康的保健作用[J].山西医药研究, 1989, 3(1-2):41. http://cdmd.cnki.com.cn/Article/CDMD-10625-2003071321.htm
[2]	RAYMAN M P. The importance of selenium to human health[J]. The Lancet, 2000, 356(9225):233-241. doi: 10.1016/S0140-6736(00)02490-9
[3]	MIZUNO M, MINATO K I, ITO H, et al. Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei Murill[J]. Biochem Mol Biol Inte, 1999, 47(4):707-714.
[4]	SORIMACHI K, AKIMOTO K, IKEHARA Y, et al. Secretion of TNF-alpha, IL-8 and nitric oxide by macrophages activated with Agaricus blazei Murill fractions in vitro[J]. Cell Structure and Function, 2001, 26(2):103-108. doi: 10.1247/csf.26.103
[5]	姜荷, 谷文英.姬松茸菌丝体多糖体外抗肿瘤活性的研究及结构初探[J].中草药, 2005, 36(11):1616-1618. doi: 10.3321/j.issn:0253-2670.2005.11.006
[6]	林戎斌, 林陈强, 张慧, 等.姬松茸食药用价值研究进展[J].食用菌学报, 2012, 19(2):117-122. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGYA201143040.htm
[7]	张惠珍, 杨淑云.姬松茸富硒培养研究[J].西南农业大学学报(S1), 2004, 6(21):95-97, 104. http://cdmd.cnki.com.cn/Article/CDMD-10625-2003071321.htm
[8]	陈彻, 郝军, 楚惠媛, 等.富硒姬松茸提取物-硒蛋白多糖处理小鼠血清对K562细胞凋亡的影响及机制[J].中国新药杂志, 2007, 16(17):1361-1364. doi: 10.3321/j.issn:1003-3734.2007.17.012
[9]	陈彻, 楚惠媛, 郝军, 等.富硒姬松茸提取物硒蛋白多糖对荷瘤小鼠的影响[J].中药材, 2006, 29(11):1215-1217. doi: 10.3321/j.issn:1001-4454.2006.11.037
[10]	刘祖同, 罗信昌.食用蕈菌生物技术及应用[M].北京:清华大学出版社, 2002.
[11]	刘新海, 冯培勇, 史亚丽, 等.姬松茸菌丝体多糖的分离纯化及抗疲劳研究[J].西南农业大学学报, 2006, 28(2):190-194. http://www.cnki.com.cn/Article/CJFDTOTAL-XNND200602004.htm
[12]	贾薇, 刘艳芳, 张劲松, 等.姬松茸菌丝体多糖提取方法初探[J].食用菌学报, 2003, 10(3):41-44. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201414044.htm
[13]	杨立红, 梁建光, 刘新海, 等.姬松茸菌丝体多糖提取工艺最优化研究[J].山东农业大学学报:自然科学版, 2004, 35(4):489-492. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNY201414044.htm
[14]	杨淑云, 刘朝贵, 朱凯, 等.富硒姬松茸液体培养条件的研究[J].微生物学通报, 2003, 30(1):9-13. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWT200301003.htm
[15]	苗敬芝, 吕兆启.灵芝深层液体发酵多糖的提取与测定[J].食品科技, 2003, (2):13-14. http://www.cnki.com.cn/Article/CJFDTOTAL-SSPJ200302004.htm
[16]	刘兴艳, 朱静平, 周浩, 等.原子荧光测定苹果中的微量硒[J].西南农业大学学报:自然科学版, 2006, 28(1):106-110. http://www.cnki.com.cn/Article/CJFDTOTAL-GXJB2012S3003.htm
[17]	黄翔宇, 程度, 黄科, 等.姬松茸菌丝体与子实体多糖的提取及含量的比较[J].广东药学, 2002, 12(3):2-4. http://www.cnki.com.cn/Article/CJFDTOTAL-YAXU200203001.htm
[18]	姜荷, 谷文英.姬松茸菌丝体中各组分多糖的抗肿瘤免疫活性[J].天然产物研究与开发, 2005, 17(2):152-156. http://cdmd.cnki.com.cn/Article/CDMD-10295-2005104904.htm