Screening and Identification of High Cellulase-producing Bacteriafrom Soil at Mulberry Fields

LUO Feng-feng; ZHANG Chang-wei; MO Ya-ling; CEN Zhong-yong

doi:10.19303/j.issn.1008-0384.2017.04.014

Volume 32 Issue 4

May 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(4): 425-430

LUO Feng-feng, ZHANG Chang-wei, MO Ya-ling, CEN Zhong-yong. Screening and Identification of High Cellulase-producing Bacteriafrom Soil at Mulberry Fields[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 425-430. doi: 10.19303/j.issn.1008-0384.2017.04.014

Citation:

LUO Feng-feng, ZHANG Chang-wei, MO Ya-ling, CEN Zhong-yong. Screening and Identification of High Cellulase-producing Bacteriafrom Soil at Mulberry Fields[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 425-430. doi: 10.19303/j.issn.1008-0384.2017.04.014

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Screening and Identification of High Cellulase-producing Bacteriafrom Soil at Mulberry Fields

doi: 10.19303/j.issn.1008-0384.2017.04.014

College of Chemical and Biological Engineering, Hechi Vniversity, Yizhou, Guang xi 546300, China

Received Date: 2016-10-05
Rev Recd Date: 2016-11-29
Publish Date: 2017-04-28

Abstract

Abstract

High cellulase-producing strains of microorganisms were screened and identified from soil at mulberry fields. Using CMC-Na as the sole carbon source, 12 target bacteria were isolated with Congo red dyeing. YZB46 was found to show the greatest cellulasesecretion among all. Preliminary analysis on thesecreted metabolitesindicated that the endo-cellulaseactivityreached 17.08 U·mL^-1 at pH 6.0 and 40 ℃, and the enzymedisplayed a desirable genetic stability. Based on the results of morphological observation, gram-staining, physiological and biochemical characterizations, as well as 16S rDNA sequencing, the isolated YZB46 appeared to beclosely related to Bacillus cereus.
- cellulase,
- screening,
- identification

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References(29)

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