Characterization and Expression Profiling of COX7C genes in Plutella xylostella L

CHEN Wei-jun; XIE Miao; ZHONG Fan; YOU Yan-chun; YOU Min-sheng

doi:10.19303/j.issn.1008-0384.2017.04.009

Volume 32 Issue 4

May 2017

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Fujian Journal of Agricultural Sciences > 2017 > 32(4): 398-402. > DOI: 10.19303/j.issn.1008-0384.2017.04.009

CHEN Wei-jun, XIE Miao, ZHONG Fan, YOU Yan-chun, YOU Min-sheng. Characterization and Expression Profiling of COX7C genes in Plutella xylostella L[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 398-402. DOI: 10.19303/j.issn.1008-0384.2017.04.009

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Characterization and Expression Profiling of COX7C genes in Plutella xylostella L

CHEN Wei-jun^{1, 2, 3, 4,},
XIE Miao^{1, 2, 3, 4},
ZHONG Fan²,
YOU Yan-chun^{1, 2, 3, 4},
YOU Min-sheng^{1, 3, 4, ,}

1.
Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3.
Fujian-Taiwan Joint Centre for Ecological Control Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
4.
Key Laboratory of Integrated Pests Management for Fujian-Taiwan Crops, Ministry of Agriculture, Fuzhou, Fujian 350002, China

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Received Date: February 05, 2017
Revised Date: March 14, 2017

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Abstract

Abstract

Cytochrome C oxidase (COX) is a member of the heme-copper oxidase superfamily, which is a terminal oxidase of eukaryotic mitochondrial intima and aerobic bacterial cell membrane respiratory electron transport chain, and is closely related to the synthesis of ATP. The COX7C gene is a subunit of the cytochrome C oxidase. In this study, the open reading frame COX7C gene of Plutella xylostella was cloned and sequenced, which includes 213 bp nucleotide. The phylogenetic tree analysis showed that COX7C was not highly conserved between the same order insects. The expression levels of COX7C in the third instar larvae of chlorpyrifos-, fipronil-resistant and susceptible strains were analyzed by qPCR. The results showed that COX7C was expressed in all three strains, and the expression level in the fipronil-resistant strain was significantly higher than that in SS, indicating that it might be involved in the process of detoxification of fipronil in P. xylostella. This study only conducted a preliminary study of COX7C in the P.xylostella, and a foundation for further research on the function of COX7C in the future.
- Plutella xylostella,
- Cytochrome Coxidase,
- Resistance,
- qPCR

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References

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