Diversity and Mercury-resistance of <i>Actinomycetes</i> in Tongren, Guizhou

LONG Yun-chuan; ZHOU Juan; ZHOU Shao-qi; WAN He-feng; LIU Zhi-rong; REN Chun-guang

doi:10.19303/j.issn.1008-0384.2017.02.021

Volume 32 Issue 2

Apr. 2017

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(2): 212-216

LONG Yun-chuan, ZHOU Juan, ZHOU Shao-qi, WAN He-feng, LIU Zhi-rong, REN Chun-guang. Diversity and Mercury-resistance of Actinomycetes in Tongren, Guizhou[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 212-216. doi: 10.19303/j.issn.1008-0384.2017.02.021

Citation:

LONG Yun-chuan, ZHOU Juan, ZHOU Shao-qi, WAN He-feng, LIU Zhi-rong, REN Chun-guang. Diversity and Mercury-resistance of Actinomycetes in Tongren, Guizhou[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 212-216. doi: 10.19303/j.issn.1008-0384.2017.02.021

Citation:

PDF( 1329 KB)

Diversity and Mercury-resistance of Actinomycetes in Tongren, Guizhou

doi: 10.19303/j.issn.1008-0384.2017.02.021

1.
Guizhou Institute of Biology, Guizhou Academy of Sciences, Guiyang, Guizhou 550009, China
2.
School of Chemistry, Biology and Material Science, East China Institute of Technology, Nanchang, Jiangxi 330013, China
3.
College of Resource and Environmental Engineering, Guizhou University, Guiyang, Guizhou 550003, China
4.
School of Environment and Energy, South China University of Technology, Guangzhou, Guangdong 510006, China

Received Date: 2016-07-23
Rev Recd Date: 2016-11-08
Publish Date: 2017-02-01

Abstract

Abstract

Diversity and mercury-resistance of Actinomycetes in the soil polluted by heavymetals at Tongren karst regeions in Guizhou were studied to gain information for environmental recovery and bioremedies. Twenty-five soil samples were collected from the areas. Microorganisms were screened on 7 different culture media with gradient concentrations of mercury under 3 different treatments to isolate the resistant strains. Subsequently, the isolates were examined for their morphological, physiological and biological characteristics, and subjected to a 16S rDNA phylogenetic an alysis for further identification.As a result, 56 typical Actinomycetes belonging to Streptomyces, Streptosporangium, Thermomonospora, Actinobacillus, Intrasporangium, Nocardia, and Micromonospora were found to be resistant to mercury.Among them, 68% were Streptomyces with one particular strain showing a high mercury-resistance to survive under a 75 mg·L^-1 up to 85 mg·L^-1 mercury stress, which was identified to be Streptomyces rameus.
- Actinomycetes,
- diversity,
- karst,
- 16S rDNA,
- mercury,
- Streptomyces rameus

FullText(HTML)

References(18)

References

[1]	彭晚霞, 王克林, 宋同清, 等.喀斯特脆弱生态系统复合退化控制与重建模式[J].生态学报, 2008, 28(2): 811-820. http://www.cnki.com.cn/Article/CJFDTOTAL-STXB200802044.htm
[2]	宋敏, 邹冬生, 杜虎, 等.不同土地利用方式下喀斯特峰丛洼地土壤微生物群落特征[J].应用生态学报, 2013, 24(9): 2471-2478. http://www.cnki.com.cn/Article/CJFDTOTAL-YYSB201309012.htm
[3]	KUMAR V, BHARTI A, GUPTA V, et al. Actinomycetes from solitary wasp mud nest and swallow bird mud nest: isolation and screening for their antibacterial activity[J]. World journal of microbiology & biotechnology, 2012, 28(3): 871-880.
[4]	PASSARI A, MISHRA V, SAIKIA R, et al. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential[J]. Frontiers in microbiology, 2015, (6): 273-278. doi: 10.3389/fmicb.2015.00273/full
[5]	姚斌, 尚鹤, 刘成志, 等.废弃柴河铅锌矿区土壤微生物特征调查研究[J].林业科学研究, 2006, 19(3): 400-403. http://www.cnki.com.cn/Article/CJFDTOTAL-LYKX200603025.htm
[6]	李慧芬, 林雁冰, 王娜娜, 等.一株Zn抗性菌株的筛选鉴定及吸附条件优化[J].环境科学学报, 2010, 30(11): 2189-2196. http://www.cnki.com.cn/Article/CJFDTOTAL-HJXX201011006.htm
[7]	HAMEDI J, DEHHAGHI M, MOHAMMDIPANAH F. Isolation of extremely heavy metal resistant strains of rare actinomycetes from high metal content soils in Iran[J]. International Journal of Environmental Research, 2015, 9(2): 475-480.
[8]	POLTI M, APARICIO J, BENIMELI C, et al. Simultaneous bioremediation of Cr (Ⅵ) and lindane in soil by actinobacteria[J]. International Biodeterioration & Biodegradation, 2014. 88: 48-55. http://www.sciencedirect.com/science/article/pii/S0964830513004411
[9]	黄代宽, 董泽琴, 刘永霞, 等.典型区域历史遗留土壤污染问题及其综合防治研究[J].环境科学与管理, 2015, 40(5): 54-58. http://www.cnki.com.cn/Article/CJFDTOTAL-BFHJ201505015.htm
[10]	彭云霞, 姜怡, 段淑蓉, 等.稀有放线菌的选择性分离方法[J].云南大学学报:自然科学版, 2007, 29(1): 86-89. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWT200601028.htm
[11]	ZHANG J, ZHANG L. Improvement of an isolation medium for actinomycetes[J]. Modern Applied Science, 2011, 5(2): 124-127.
[12]	阮继生.放线菌分类基础[M].北京:科学出版社, 1992. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm
[13]	中国科学院微生物研究所.链霉菌鉴定手册[M].北京:科学出版社, 1975. http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm
[14]	PEREIRA S, LIMA A, FIGUEIRA E. Screening possible mechanisms mediating cadmium resistance in Rhizobium leguminosarum bv. viciae isolated from contaminated Portuguese soils[J]. Microbial ecology, 2006, 52(2): 176-186. doi: 10.1007/s00248-006-9057-5
[15]	安德荣, 慕小倩, 刘翠娟, 等.土壤拮抗放线菌的分离和筛选[J].微生物学杂志, 2002, 22(5): 1-3. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWX200205000.htm
[16]	马爱爱, 徐世健, 敏玉霞, 等.祁连山高山植物根际土放线菌生物多样性[J].生态学报, 2014, 34(11): 2016-2028. http://www.cnki.com.cn/Article/CJFDTOTAL-STXB201411014.htm
[17]	曾艳, 陈强, 王敏, 等.一株高抗汞细菌的分离鉴定及其抗性基因的克隆与表达[J].微生物学报, 2009, 49(12): 1628-1633. doi: 10.3321/j.issn:0001-6209.2009.12.013
[18]	XU H, CAO D, TIAN Z. Isolation and identification of a mercury resistant strain[J]. Environment Protection Engineering, 2012, 38(4): 67-74.