Screening and Culture of a Strain of <i>Metarhizium</i> Highly Pathogenic to <i>Phyllotreta striolata</i>

HE Yue-chao; CHEN Jiang; SHI Meng-zhu; LI Jian-yu; WANG Ting; FU Jian-wei; WU Mei-xiang

doi:10.19303/j.issn.1008-0384.2017.02.017

Volume 32 Issue 2

Apr. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(2): 189-194

HE Yue-chao, CHEN Jiang, SHI Meng-zhu, LI Jian-yu, WANG Ting, FU Jian-wei, WU Mei-xiang. Screening and Culture of a Strain of Metarhizium Highly Pathogenic to Phyllotreta striolata[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 189-194. doi: 10.19303/j.issn.1008-0384.2017.02.017

Citation:

HE Yue-chao, CHEN Jiang, SHI Meng-zhu, LI Jian-yu, WANG Ting, FU Jian-wei, WU Mei-xiang. Screening and Culture of a Strain of Metarhizium Highly Pathogenic to Phyllotreta striolata[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 189-194. doi: 10.19303/j.issn.1008-0384.2017.02.017

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Screening and Culture of a Strain of Metarhizium Highly Pathogenic to Phyllotreta striolata

doi: 10.19303/j.issn.1008-0384.2017.02.017

HE Yue-chao^{1, 2, 3
,},
CHEN Jiang^{1, 2},
SHI Meng-zhu²,
LI Jian-yu²,
WANG Ting²,
FU Jian-wei^{2, 3, 4
,
,},
WU Mei-xiang^{1
,
,}

1.
College of Plant Protection, Fujian Agriculture and Forest University, Fuzhou, Fujian 350002, China
2.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fuzhou, Fujian 350013, China
3.
Fujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
4.
Institute of Quality Standards & Testing Technology for Agro-Products, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350013, China

Received Date: 2016-10-02
Rev Recd Date: 2016-11-12
Publish Date: 2017-02-01

Abstract

Abstract

This study attempted to find a microorganism that could be effectively used for the bio-control of the major pestinsect, Phyllotreta striolata, on vegetables.Metarhizium anisopliae is known as apathogenic fungus that infects the pest. This experimentation evaluated the virulence of 8 M. anisopliae strains against P. striolata. One strainthat exhibited a high activity was Ma6. The bioassay showed a LT₅₀ on adult P. striolata inoculated with 8.0×10⁸ Ma6 spores·mL^-1 at 25℃ to be 4.09 d, and a 100% cumulative mortality rateat 25℃ for 7 d. It was, thus, concluded that Ma6 could be a potential candidate for the pest control. Subsequently, studies showed that the optimum medium for the hyphal growth and sporulation of Ma6 was PDA at 25℃ and pH 7.0 with 6 d in the dark followed by 8 d of sun exposure.
- bio-control,
- pathogenicity,
- cultural conditions,
- sporulation condition

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References(21)

References

[1]	宋艳霞, 董易之, 张茂新.黄曲条跳甲为害引起的菜心价值损失及防治指标研究[J].华南农业大学学报, 2011, 32(1): 53-56. doi: 10.7671/j.issn.1001-411X.2011.01.012
[2]	BURGESS L. Flea beetles (Coleoptera: Chysomelidae) attacking rape crops in the Canadian Prarie Provinces [J].Canadian Entomologist, 1977, 109: 21-32. doi: 10.4039/Ent10921-1
[3]	张茂新, 凌冰.黄曲条跳甲防治技术研究新进展[J].植物保护, 2000, 26(6): 31-33. http://www.cnki.com.cn/Article/CJFDTOTAL-ZWBH200006014.htm
[4]	杨建云, 纪春艳, 凌冰, 等.黄曲条跳甲幼虫致病菌的鉴定及其对黄曲条跳甲的杀虫活性研究[J].中国生物防治学报, 2014, 30(3): 434-440. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201403025.htm
[5]	傅建炜, 李建宇, 邱良妙, 等.福建省黄曲条跳甲药剂敏感性的地区差异[J].福建农林大学学报:自然科学版, 2006, 35(3): 235-238. http://www.cnki.com.cn/Article/CJFDTOTAL-FJND200603002.htm
[6]	黄运霞, 黄荣瑞, 李焜华.坚强芽孢杆菌对黄条跳甲的毒效初步试验[J].生物防治通报, 1992, 8(4): 182. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF199204017.htm
[7]	邝灼彬, 吕利华, 冯夏, 等.球孢白僵茵对四种十字花科蔬菜害虫的兼控潜力评价[J].昆虫知识, 2005, 42(6): 673-676. doi: 10.7679/j.issn.2095-1353.2005.168
[8]	张彦丰, 王正浩, 农向群, 等.绿僵菌侵染对东亚飞蝗中肠保护酶和解毒酶的影响[J].中国生物防治学报, 2015, 31(6):876-881. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201506010.htm
[9]	高松.绿僵菌研究的新进展[J].中国生物防治, 1996, 12(4): 182-187. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF604.010.htm
[10]	王鹏, 张亚波, 舒金平, 等.金龟子绿僵菌小孢变种对筛胸梳爪叩甲幼虫致病力的生物测定[J].中国生物防治, 2010, 26(3): 274-279. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201003006.htm
[11]	何学友, 蔡守平, 童应华, 等.球孢白僵菌和金龟子绿僵菌不同菌株对黑足角胸叶甲成虫的致病力评价[J].昆虫学报, 2011, 54(11): 1281-1287. http://www.cnki.com.cn/Article/CJFDTOTAL-KCXB201111010.htm
[12]	ZIMMERMANN G. The entomopathogenic fungus Metar-hizium anisopliae and its potential as a biocontrol agent [J]. Pesticide Science, 1993, 37: 375-379. doi: 10.1002/ps.2780370410
[13]	张亚波, 叶碧欢, 吴小双, 等.一株虫生真菌的分离、鉴定及其对筛胸梳爪叩甲幼虫致病力测定[J].中国生物防治学报, 2015, 31(6):868-875. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201506009.htm
[14]	张建伟.小菜蛾高致病力绿僵菌的筛选、鉴定及培养特性研究[J].中国生物防治学报, 重庆大学, 2011, 28(1):53-61. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201201010.htm
[15]	刘加妹, 彭景楩.生物实验数据的单因素方差分析[J].动物学杂志, 2001, 36(6): 34-37. http://www.cnki.com.cn/Article/CJFDTOTAL-BIRD200106007.htm
[16]	李海龙, 张桂林, 党荣理, 等.应用SPSS软件进行杀虫剂的KT₅₀分析[J].中国媒介生物学及控制杂志, 2011, 22(2), 155-157.
[17]	王海川, 尤民生.绿僵菌对昆虫的入侵机理[J].微生物学通报, 1999, (1): 71-73. http://www.cnki.com.cn/Article/CJFDTOTAL-WSWT199901029.htm
[18]	ARTHURS S, THOMAS M B. Effect of temperature and relative humidity on sporulation of Metarhizium anisopliae var. acridum in mycosed cadavers of Schistocerca gregaria[J]. Journal of Invertebrate Pathology, 2001, 78: 59-65. doi: 10.1006/jipa.2001.5050
[19]	陈自宏, 徐玲, 杨凤连, 等.绿僵菌菌株MAX-2鉴定及其抗逆性特性研究[J].云南农业大学学报, 2014, 29(6): 834-839. http://www.cnki.com.cn/Article/CJFDTOTAL-YNDX201406009.htm
[20]	殷幼平, 宋章永, 谢宁, 等.金龟子绿僵菌CQM117的营养要求与培特性初探[J].中国生物防治, 2010, 26(2): 206-210. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201002016.htm
[21]	孙家宝, 邓勋, 宋小双.金龟子绿僵菌的生物学特性及毒力测定[J].中国林副特产, 2007, (3): 17-19. http://www.cnki.com.cn/Article/CJFDTOTAL-CTFL200703006.htm