Optimization by Response Surface Methodology for <i>Agaricus bisporus</i> W38 Fermentation in Liquid Medium

ZENG Zhi-heng; CHENG Yi; ZENG Hui; YANG Lei; LIAO Jian-hua; SHI Xiao-kun; CAI Zhi-ying; DAI Jian-qing

doi:10.19303/j.issn.1008-0384.2017.02.011

Volume 32 Issue 2

Apr. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(2): 161-166

ZENG Zhi-heng, CHENG Yi, ZENG Hui, YANG Lei, LIAO Jian-hua, SHI Xiao-kun, CAI Zhi-ying, DAI Jian-qing. Optimization by Response Surface Methodology for Agaricus bisporus W38 Fermentation in Liquid Medium[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 161-166. doi: 10.19303/j.issn.1008-0384.2017.02.011

Citation:

ZENG Zhi-heng, CHENG Yi, ZENG Hui, YANG Lei, LIAO Jian-hua, SHI Xiao-kun, CAI Zhi-ying, DAI Jian-qing. Optimization by Response Surface Methodology for Agaricus bisporus W38 Fermentation in Liquid Medium[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 161-166. doi: 10.19303/j.issn.1008-0384.2017.02.011

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Optimization by Response Surface Methodology for Agaricus bisporus W38 Fermentation in Liquid Medium

doi: 10.19303/j.issn.1008-0384.2017.02.011

ZENG Zhi-heng^{1, 2
,},
CHENG Yi^{1, 2},
ZENG Hui^{1, 2, 3},
YANG Lei^{1, 2},
LIAO Jian-hua^{1, 2},
SHI Xiao-kun^{1, 2},
CAI Zhi-ying⁴,
DAI Jian-qing^{1, 2
,
,}

1.
Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China
2.
National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fuzhou, Fujian 350014, China
3.
Engineering Research Center of Chinese Ministry Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jinlin 130118, China
4.
Edible Fungi Station of Longhai Agriculture Bureau, Longhai, Fujian 363199, China

Received Date: 2016-09-12
Rev Recd Date: 2016-12-14
Publish Date: 2017-02-01

Abstract

Abstract

Based on the results obtained by a previous single factor test, medium formulation and conditions for the fermentation of Agaricus bisporus W38 were optimized by the Plackett-Burman experimental design and the central composite design with Design-Expert software. A regression equation was obtained using the response surface methodology. The optimized medium was formulated toconsistof 39.90 g·L^-1, 13.60 g·L^-1, K₂HPO₄ 2.0 g·L^-1, and MgSO₄·7H₂O 1.067 5 g·L^-1. The fermentation was designed to be carried out with an initial pH of 6.5 and maintained at 24℃ with a constant shaking in flask at 180 r·min^-1 for 7 d. A mycelial biomass 100 mL of 2.35 g, which was 3.05-foldof the original design, and an average mycelial growth rate improvement by 14.7% were obtained.
- Agaricus bisporus,
- liquid fermentation,
- response surface methodology,
- optimization

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References(9)

References

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