Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers

NING Lin; CHEN Hao-jun; HE Jiang; YANG Xiang-yan; YAN Lin; TANG Yu-juan

doi:10.19303/j.issn.1008-0384.2017.02.007

Volume 32 Issue 2

Apr. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(2): 138-143

NING Lin, CHEN Hao-jun, HE Jiang, YANG Xiang-yan, YAN Lin, TANG Yu-juan. Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 138-143. doi: 10.19303/j.issn.1008-0384.2017.02.007

Citation:

NING Lin, CHEN Hao-jun, HE Jiang, YANG Xiang-yan, YAN Lin, TANG Yu-juan. Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers[J]. Fujian Journal of Agricultural Sciences, 2017, 32(2): 138-143. doi: 10.19303/j.issn.1008-0384.2017.02.007

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Genetic Relationship among Guava Germplasms Accessed by Using ISSR Markers

doi: 10.19303/j.issn.1008-0384.2017.02.007

Guangxi Subtropical Crops Research Institute, Nanning, Guangxi 530001, China

Received Date: 2016-07-26
Rev Recd Date: 2016-10-08
Publish Date: 2017-02-01

Abstract

Abstract

Using ISSR molecular markers, PCR products of 36 guava germplasms were purified by polyacrylamide gel electrophoresis and photographed the images for a UPGMA analysis to establish the genetic relationship. Out of 100 UBC series ISSR primers, 9 rich and stable polymorphic primers were selected and 165 bands amplified. Among the 165 bands, 129 were polymorphic rendering a polymorphism percentage of 78.18%. The amplified results could satisfactorily distinguish the 36 germplasms. The genetic similarity coefficient of all samples ranged from 0.70 to 0.93, averaging 0.815. At the coefficient of 0.75, the 36 germplasms were divided into 4 groups. The results were consistent with the morphological classification. Although the genetic basis for classification was weak, with the aid of ISSR molecular markers, clear identifications for the individual germplasms of the collection were obtained.
- guava,
- ISSR,
- genetic relationship

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References(24)

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