Optimizing Solid-medium Fermentation of Non-pathogenic <i>Fusarium oxysporum</i>

XIAO Rong-feng; CHENG Yan-ping; LIU Bo; GE Ci-bin; ZHU Yu-jing

doi:10.19303/j.issn.1008-0384.2017.012.014

Volume 32 Issue 12

Feb. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(12): 1350-1353

XIAO Rong-feng, CHENG Yan-ping, LIU Bo, GE Ci-bin, ZHU Yu-jing. Optimizing Solid-medium Fermentation of Non-pathogenic Fusarium oxysporum[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1350-1353. doi: 10.19303/j.issn.1008-0384.2017.012.014

Citation:

XIAO Rong-feng, CHENG Yan-ping, LIU Bo, GE Ci-bin, ZHU Yu-jing. Optimizing Solid-medium Fermentation of Non-pathogenic Fusarium oxysporum[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1350-1353. doi: 10.19303/j.issn.1008-0384.2017.012.014

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Optimizing Solid-medium Fermentation of Non-pathogenic Fusarium oxysporum

doi: 10.19303/j.issn.1008-0384.2017.012.014

Agricultural Bio-resources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2017-01-16
Rev Recd Date: 2017-09-05
Publish Date: 2017-12-28

Abstract

Abstract

Optimal culture conditions on solid-medium for the non-pathogenic Fusarium oxysporum FJAT-9290 to produce vaccine against fusarium wilt were investigated. The initial moisture content and inoculum amount in the medium, as well as the temperature and duration for the fermentation were varied to determine their effects on the resulting yield of conidia. It appeared that, a medium with 50% moisture in a tissue culture bottle inoculated with 7% of FJAT-9290 to ferment at 28℃ for 7 ds followed by cultivation in a polypropylene bag could produce a maximized conidium count of 6.35×10⁸·g^-1 on the 20^th day. The condia count quadrupled that of the initial inoculum.
- non-pathogenic Fusarium oxysporum,
- solid-medium fermentation,
- fermentation condition

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References(18)

References

[1]	SHISHIDO M, MIWA C, USAMI T, et al. Biological control efficiency of Fusarium wilt of tomato by nonpathogenic Fusarium oxysporum Fo-B2 in different environments[J].Phytopathology, 2005, 95(9):1072-1080. doi: 10.1094/PHYTO-95-1072
[2]	VELOSO J, DÍAZ J.Fusarium oxysporum Fo47 confers protection to pepper plants against Verticillium dahliae and Phytophthora capsici, and induces the expression of defence genes[J].Plant Pathology, 2012, 61(2):281-288. doi: 10.1111/ppa.2012.61.issue-2
[3]	PU X M, XIE B Y, LI P Q, et al.Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20[J]. FEMS Microbiology Letters, 2014, 355(2):142-151. doi: 10.1111/fml.2014.355.issue-2
[4]	曾庆才, 肖荣凤, 刘波, 等.生防菌哈茨木霉FJAT-9040固体发酵条件的响应面优化[J].福建农业学报, 2015, 30(2):192-197. http://www.fjnyxb.cn/CN/abstract/abstract2635.shtml
[5]	张宗耀, 梁关海, 梁蕾, 等.培养基及培养条件对冬虫夏草菌固体发酵产分生孢子的影响[J].菌物学报, 2016, 35(4):440-449. http://www.docin.com/p-1549121438.html
[6]	刘波, 黄俊生, 肖荣凤, 等.尖孢镰刀菌生物学及其生物防治[M].北京:科学出版社, 2013:53-56.
[7]	王永东, 蒋立科, 岳永德, 等.生防菌株哈茨木霉H-13固体发酵条件的研究[J].浙江大学学报:农业与生命科学版, 2006, 32(6):645-650. http://industry.wanfangdata.com.cn/dl/Detail/Thesis?id=Thesis_Y1106746
[8]	肖荣凤, 刘波, 唐建阳, 等.哈茨木霉FJAT-9040生防菌剂固体发酵及其对苦瓜枯萎病的防治效果[J].中国生物防治学报, 2015, 31(4):508-515. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSWF201504011.htm
[9]	李浪, 杨旭, 薛永亮.现代固态发酵技术工艺、设备及应用研究进展[J].河南工业大学学报:自然科学版, 2011, 32(1):89-94. https://www.wenkuxiazai.com/doc/f795078acc22bcd126ff0c81.html
[10]	赵国鹏, 席北斗, 夏训峰, 等.固态发酵技术及其在农产品加工业废渣处理中的应用[J].环境工程技术学报, 2012, 2(5):441-448. http://www.cnki.com.cn/Article/CJFDTOTAL-HKWZ201205018.htm
[11]	周俊萍, 杨本寿, 左详, 等.一株广谱抑菌活性内生真菌的鉴定及其固体发酵条件的优化[J].云南农业大学学报:自然科学版, 2014, 29(5):766-772. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=yndx201405026&dbname=CJFD&dbcode=CJFQ
[12]	肖荣凤, 刘波, 朱育菁, 等.非致病性尖孢镰刀菌FJAT-9290的定殖特性及对番茄枯萎病的防治效果[J].植物保护学报, 2015, 42(2):169-175. http://www.doc88.com/p-9793791357171.html
[13]	肖荣凤, 郑梅霞, 刘波, 等.利用响应面法优化非致病性尖孢镰刀菌FJAT-9290固体发酵培养基[J].中国生物防治学报, 2017, 33(2):258-265. https://www.cnki.com.cn/qikan-ZSWF201602016.html
[14]	PIO T F, MACEDO G A.Optimizing the production of cutinase by Fusarium oxysporum, using response surface methodology[J].Enzyme and Microbial Technology, 2007, 41(5):613-619. doi: 10.1016/j.enzmictec.2007.05.008
[15]	PARMAR P, OZA V P, SUBRAMANIAN R B.Optimization of fusaric acid production by Fusarium oxysporum f.sp.lycopersici using response surface methodology[J].Indian Journal of Science and Technology, 2010, 3(4):411-416. http://www.freepatentsonline.com/y2016/0355797.html
[16]	庄义庆, 乔广行, 王源超, 等.蕉斑镰刀菌3-26菌株产孢条件研究[J].南京农业大学学报, 2008, 31(4):77-81. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=njny200804016&dbname=CJFD&dbcode=CJFQ
[17]	罗洋, 滕应, 刘方, 等.里氏木霉FS10-C固体发酵基质筛选及发酵条件初探[J].生物技术通报, 2014, 30(3):111-116. http://biotech.caas.cn/EN/article/downloadArticleFile.do?attachType=PDF&id=10180
[18]	谷军, 张晓彦, 沙长青.淡紫拟青霉固态发酵工艺优化[J].生物技术, 2014, 24(6):94-98. http://www.doc88.com/p-69911711909.html