Isolation and Preliminary Identification on Pathogen of Black Rot Disease on Non-heading Chinese Cabbages in Fuzhou

ZHENG Xue-li; XIE Xin-xin; LIN Feng; SHAO Gui-rong

doi:10.19303/j.issn.1008-0384.2017.012.012

Volume 32 Issue 12

Feb. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(12): 1335-1340

ZHENG Xue-li, XIE Xin-xin, LIN Feng, SHAO Gui-rong. Isolation and Preliminary Identification on Pathogen of Black Rot Disease on Non-heading Chinese Cabbages in Fuzhou[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1335-1340. doi: 10.19303/j.issn.1008-0384.2017.012.012

Citation:

ZHENG Xue-li, XIE Xin-xin, LIN Feng, SHAO Gui-rong. Isolation and Preliminary Identification on Pathogen of Black Rot Disease on Non-heading Chinese Cabbages in Fuzhou[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1335-1340. doi: 10.19303/j.issn.1008-0384.2017.012.012

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Isolation and Preliminary Identification on Pathogen of Black Rot Disease on Non-heading Chinese Cabbages in Fuzhou

doi: 10.19303/j.issn.1008-0384.2017.012.012

Institute of Vegetable Science of Fuzhou, Fuzhou, Fujian 350111, China

Received Date: 2017-06-28
Rev Recd Date: 2017-10-23
Publish Date: 2017-12-28

Abstract

Abstract

Pathogen that caused the black rot disease on non-heading Chinese cabbages in Fuzhou was investigated. Microbial samples were collected from the diseased plants for isolation. Based on the morphology and inoculation test, selected microorganisms were tested further. Their 16S rDNA were amplified by PCR with the universal primer 16S. The positive fragment was sequenced and analyzed against NCBI database. A 99.8% homology was found between an isolated bacterium and Xanthomonas campestris pv. campestris (Genbank:CP017308.1). Subsequently, the 16S rDNA sequence of the strain from the infected cabbage was confirmed to be identical to that of the pathogen for the inoculation. It was, therefore, concluded that the isolated strain was indeed the pathogen that caused the black rot disease on the non-heading cabbages, and was preliminary identified as Xanthomonas campestris pv. campestris.
- non-heading Chinese cabbage,
- black rot disease,
- 16S rDNA,
- pathogenicity

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References(30)

References

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