Diagnosis of Duckling Short Beak and Dwarfism Syndrome by Indirect Immunofluorescence Assay

YU Bo; ZHU Xiao-li; CHENG Xiao-xia; CHEN Shao-ying

doi:10.19303/j.issn.1008-0384.2017.012.011

Volume 32 Issue 12

Feb. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(12): 1332-1334

YU Bo, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shao-ying. Diagnosis of Duckling Short Beak and Dwarfism Syndrome by Indirect Immunofluorescence Assay[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1332-1334. doi: 10.19303/j.issn.1008-0384.2017.012.011

Citation:

YU Bo, ZHU Xiao-li, CHENG Xiao-xia, CHEN Shao-ying. Diagnosis of Duckling Short Beak and Dwarfism Syndrome by Indirect Immunofluorescence Assay[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1332-1334. doi: 10.19303/j.issn.1008-0384.2017.012.011

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Diagnosis of Duckling Short Beak and Dwarfism Syndrome by Indirect Immunofluorescence Assay

doi: 10.19303/j.issn.1008-0384.2017.012.011

YU Bo^{1, 2},
ZHU Xiao-li^{2, 3},
CHENG Xiao-xia^{2, 3},
CHEN Shao-ying^{2, 3
,
,}

1.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
3.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China

Received Date: 2017-05-22
Rev Recd Date: 2017-09-26
Publish Date: 2017-12-28

Abstract

Abstract

An indirect immunofluorescence assay (IFA) was established using the monoclonal antibodies (Mab E16) against goose parvovirus (GPV) for identification of the short beak and dwarfism syndrome (SBDS)-GPV in ducks. Liver, spleen, kidney and pancreas of the ducklings challenged with SBDS-GPV and the natural infection cases were tested by the new IFA methodology. The results showed that the newly developed IFA was specific, giving positive reaction to GPV only and negative to other viruses of MPV, MDRV, NDRV, DHV, DPV and PMV. Five out of 5 challenged cases and 11 out of 12 natural infection cases showed a positive reaction with bright green fluorescence glow on IFA. Consequently, the new method could be applicable for detecting and diagnosing SBDS in ducks with high specificity, accuracy and rapidity.
- short beak and dwarfism syndrome,
- monoclonal antibody against goose parvovirus,
- indirect immunofluorescence assay,
- diagnosis

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References(10)

References

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