Genomic Library Construction of Monokaryons with Different Mating Type of <i>Agaricus bisporus</i>

CAI Zhi-Xin; CHEN Mei-Yuan; LIAO Jian-Hua; LI Hong-Rong; GUO Zhong-Jie; WANG Ze-Sheng

doi:10.19303/j.issn.1008-0384.2017.012.010

Volume 32 Issue 12

Feb. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(12): 1327-1331

CAI Zhi-Xin, CHEN Mei-Yuan, LIAO Jian-Hua, LI Hong-Rong, GUO Zhong-Jie, WANG Ze-Sheng. Genomic Library Construction of Monokaryons with Different Mating Type of Agaricus bisporus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1327-1331. doi: 10.19303/j.issn.1008-0384.2017.012.010

Citation:

CAI Zhi-Xin, CHEN Mei-Yuan, LIAO Jian-Hua, LI Hong-Rong, GUO Zhong-Jie, WANG Ze-Sheng. Genomic Library Construction of Monokaryons with Different Mating Type of Agaricus bisporus[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1327-1331. doi: 10.19303/j.issn.1008-0384.2017.012.010

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Genomic Library Construction of Monokaryons with Different Mating Type of Agaricus bisporus

doi: 10.19303/j.issn.1008-0384.2017.012.010

Institute of Edible Fungi, Fujian Academy of Agricultural Sciences/National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China

Received Date: 2017-10-11
Rev Recd Date: 2017-12-20
Publish Date: 2017-12-28

Abstract

Abstract

In this study, twenty-two Agaricus bisporus monokaryons strains were pairwisely paired.Based on strain 9608, two different mating types of monokaryons were obtained.A + strains:9601, 9608, 9609, 9612, AgQG841-1, AgLH830-4, AgLH830-6, AgQL8125-5.A-:9602, 9603, 9604, 9607, Ag2k811-1, M7206-1, M7206-2, M7206-11, M7206-13.The genomic DNA of two strains were extracted and DNA library were constructed.The library was verified by restriction enzyme digestion with SalⅠ.The 20 kb, 14 kb and 9 kb 3 fragments were cut out, of which 20 kb and 9 kb were the inserted into the left and right arms of lambda vector and the fragment of 14kb, was the insertion genomic DNA fragments.Enzyme digestion result was in line with expectations, indicating the higher quality of the library.
- Agaricus bisporus,
- monokaryons,
- mating type factor,
- genomic library

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References(9)

References

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