ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan

WU Tian; LAN Zeng-quan; WANG Hua-fang

doi:10.19303/j.issn.1008-0384.2017.012.005

Volume 32 Issue 12

Feb. 2018

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WU Tian, LAN Zeng-quan, WANG Hua-fang. ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1298-1302. doi: 10.19303/j.issn.1008-0384.2017.012.005

Citation:

WU Tian, LAN Zeng-quan, WANG Hua-fang. ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan[J]. Fujian Journal of Agricultural Sciences, 2017, 32(12): 1298-1302. doi: 10.19303/j.issn.1008-0384.2017.012.005

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ISSR-based Genetic Analysis on Relationship of Teas in Central Yunnan

doi: 10.19303/j.issn.1008-0384.2017.012.005

WU Tian^1
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LAN Zeng-quan^{2
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WANG Hua-fang^{3
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1.
College of Horticulture and Gardening, Southwest Forestry University, Kunming, Yunnan 650224, China
2.
College of Environment Science and Engineering, Southwest Forestry University, Kunming, Yunnan 650224, China
3.
College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China

Received Date: 2017-01-17
Rev Recd Date: 2017-10-18
Publish Date: 2017-12-28

Abstract

Abstract

The genetic relationship among 17 representative tea cultivars from various regions in central Yunnan was analyzed using ISSR markers.Out of the randomly selected 28 primers in testing DNA polymorphism of the teas, 15 produced distinctive bands and were chosen for amplification.The ISSR-PCR amplified primers showed 112 stable and clearly defined bands.Of which, 103 were polymorphic (a rate of 91.96%) averaging 7.4 bands each.The GS scores on the cultivars ranged from 0.58 to 0.80 with discrete classifications of Group Ⅰ that consisted of 12 germplasms and Group Ⅱ that composed of the remaining 5 germplasms.
- central Yunnan,
- tea cultivars,
- ISSR-PCR,
- genetic relationship

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References(19)

References

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