Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different <i>Cordyceps cicadae</i> Strains

LONG Liang-kun; HUANG Shu-hui; SHI Zi-yu; LIN Qun-ying; LI Chuan-hua; DING Shao-jun

doi:10.19303/j.issn.1008-0384.2017.011.017

Volume 32 Issue 11

Jan. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(11): 1257-1262

LONG Liang-kun, HUANG Shu-hui, SHI Zi-yu, LIN Qun-ying, LI Chuan-hua, DING Shao-jun. Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different Cordyceps cicadae Strains[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1257-1262. doi: 10.19303/j.issn.1008-0384.2017.011.017

Citation:

LONG Liang-kun, HUANG Shu-hui, SHI Zi-yu, LIN Qun-ying, LI Chuan-hua, DING Shao-jun. Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different Cordyceps cicadae Strains[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1257-1262. doi: 10.19303/j.issn.1008-0384.2017.011.017

Citation:

LONG Liang-kun, HUANG Shu-hui, SHI Zi-yu, LIN Qun-ying, LI Chuan-hua, DING Shao-jun. Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different Cordyceps cicadae Strains[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1257-1262. doi: 10.19303/j.issn.1008-0384.2017.011.017

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Molecular Identification and Productivity on Hyaluronic Acid in Submerged Culture of Different Cordyceps cicadae Strains

doi: 10.19303/j.issn.1008-0384.2017.011.017

1.
Nanjing Forestry University, College of Chemical Engineering, Nanjing, Jiangsu 210037, China
2.
Nanjing Institute for the Comprehensive Utilization of Wild Plants, Nanjing, Jiangsu 210042, China
3.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences/Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture/National Engineering Research Center of Edible Fungi/Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201403, China

Received Date: 2017-07-31
Rev Recd Date: 2017-10-15
Publish Date: 2017-11-28

Abstract

Abstract

This study aimed to identify 4 Cordyceps cicadae strains and compare their productivities on hyaluronic acid (HA) in a liquid culture. The molecular identification for CC1502, CC1504, CC1601 and CC1602 was conducted based on their internal transcribed spacer (ITS) sequences and using the randomly amplified polymorphic DNA (RAPD) analysis. The results indicated that there was no difference on ITS sequences among these fungal strains, and that the RAPD patterns were amplified by the primer, AP-A20, AP-D18 or AP-H18. As shown by the dendrogram analysis with UPGMA method, CC1504 had a low genetic similarity with any of the other strains, and CC1601 related closely to CC1602. The liquid fermentation of the strains showed different levels of HA productivity between CC1502 and CC1504, and glucose and peptone were the choice carbon and nitrogen sources. The maximum HA production was up to 2.45 mg·mL^-1 generated by CC1502, which, therefore, was considered a candidate for industrial application in the future.
- Cordyceps cicadae,
- randomly amplified polymorphic DNA,
- liquid fermentation,
- hyaluronic acid

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References(18)

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