Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea

CHEN Jing; ZHENG Zhi-lin; LIN Yi; CAO Hong-li; CHEN Di; CHEN Gui-xin; YE Nai-xing

doi:10.19303/j.issn.1008-0384.2017.011.007

Volume 32 Issue 11

Jan. 2018

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CHEN Jing, ZHENG Zhi-lin, LIN Yi, CAO Hong-li, CHEN Di, CHEN Gui-xin, YE Nai-xing. Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1201-1206. doi: 10.19303/j.issn.1008-0384.2017.011.007

Citation:

CHEN Jing, ZHENG Zhi-lin, LIN Yi, CAO Hong-li, CHEN Di, CHEN Gui-xin, YE Nai-xing. Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1201-1206. doi: 10.19303/j.issn.1008-0384.2017.011.007

Citation:

CHEN Jing, ZHENG Zhi-lin, LIN Yi, CAO Hong-li, CHEN Di, CHEN Gui-xin, YE Nai-xing. Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1201-1206. doi: 10.19303/j.issn.1008-0384.2017.011.007

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Selection and Validation of Reference Genes for Real-time Florescence Quantitative PCR Analysis on Gene Expression of White Tea

doi: 10.19303/j.issn.1008-0384.2017.011.007

1.
College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou, Fujian 350002, China
2.
Department of biotechnology, Ningde Vocational and Technical College, Fu'an, Fujian 355000, China

Received Date: 2017-08-28
Rev Recd Date: 2017-09-20
Publish Date: 2017-11-28

Abstract

Abstract

To select appropriate reference genes to be used in the real-time florescence quantitative PCR analysis for studying the gene expression in of white tea leaves during withering, the software of GeNorm, NormFinder, and BestKeeper were applied. Three stable reference genes, β-Actin, GAPDH and RUBP, were identified. Among them, RUBP was found most adequate for comparing the expressions at various stages of withering, while β-Actin for differentiating them in leaves from different parts of a tea plant. The combination of GAPDH+RUBP was determined as choice reference genes for the analysis.
- white tea,
- withering,
- reference genes,
- stability,
- real-time fluorescence quantitative PCR

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References(19)

References

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