Establishment of One-step RT-PCR for Batai Virus Detection in Ducks

FU Guang-hua; CHEN Cui-teng; FU Qiu-ling; LIU Rong-chang; CHENG Long-fei; SHI Shao-hua; WAN Chun-he; CHEN Hong-mei; HUANG Yu

doi:10.19303/j.issn.1008-0384.2017.011.005

Volume 32 Issue 11

Jan. 2018

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(11): 1193-1196

FU Guang-hua, CHEN Cui-teng, FU Qiu-ling, LIU Rong-chang, CHENG Long-fei, SHI Shao-hua, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Establishment of One-step RT-PCR for Batai Virus Detection in Ducks[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1193-1196. doi: 10.19303/j.issn.1008-0384.2017.011.005

Citation:

FU Guang-hua, CHEN Cui-teng, FU Qiu-ling, LIU Rong-chang, CHENG Long-fei, SHI Shao-hua, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Establishment of One-step RT-PCR for Batai Virus Detection in Ducks[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1193-1196. doi: 10.19303/j.issn.1008-0384.2017.011.005

Citation:

FU Guang-hua, CHEN Cui-teng, FU Qiu-ling, LIU Rong-chang, CHENG Long-fei, SHI Shao-hua, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Establishment of One-step RT-PCR for Batai Virus Detection in Ducks[J]. Fujian Journal of Agricultural Sciences, 2017, 32(11): 1193-1196. doi: 10.19303/j.issn.1008-0384.2017.011.005

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Establishment of One-step RT-PCR for Batai Virus Detection in Ducks

doi: 10.19303/j.issn.1008-0384.2017.011.005

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Center/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou, Fujian 350013, China

Received Date: 2017-05-26
Rev Recd Date: 2017-09-26
Publish Date: 2017-11-28

Abstract

Abstract

For a rapid detection of Batai virus (BATV) in ducks, a one-step reverse transcription polymerase chain reaction (RT-PCR) assay was developed based on the conserved region of the M gene in BATV. With the optimized reaction conditions, this assay could specifically amplify a 480 bp fragment from the M gene. There were no similar gene amplifications on 5 other pathogens found in ducks, such as avian Tembusu virus and duck hepatitis virus. The testing sensitivity of the method was 1×10^1.1 TCID₅₀ with a high reproducibility. Consequently, this newly developed methodology seemed to be highly specific and sensitive for BATV detection, and could be applied for rapid and early diagnosis as well as epidemiological investigations on BATV.
- batai virus,
- duck,
- RT-PCR

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References(11)

References

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